2007 Fiscal Year Final Research Report Summary
Development of ultrasound spot-irradiation system with real-time optical monitoring for biological applications
| Project/Area Number |
18500352
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Meiji University |
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Principal Investigator |
HIRAOKA Wakako Meiji University, School of Science and Technology, Associate Professor (00212168)
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| Co-Investigator(Kenkyū-buntansha) |
CHOI Pak-kon Meiji University, School of Science and Technology, Professor (30143530)
KONDO Takashi University of Toyama, Faculty of Medicine, Professor (40143937)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Biophysics / ultrasound / fluorescent dye / sonodynamic reagent / cell culture / sonodynamic therapy / caged drug / hydrophone |
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| Research Abstract |
We have tried to develop an ultrasound spot-irradiation system with real-time optical monitoring for biological applications. Ultrasound has been used for various physical and chemical purposes for long time. In addition, therapeutic application of ultrasound has been a focus of constant attention recently. In this study, we made an ultrasound spot-irradiation system in which irradiated cells or tissues were monitored under microscopic stage. This system also equipped with micromanipulation and injection for microsurgery of cells and tissues. As a apparatus of ultrasound, we applied several types of piezzoceramic focusing of transducer for the range from 1 MHz to 7 MHz. These apparatus was designed for both continuous and burst wave. The power of ultrasound was determined by calorimetry.
For the development of new application with this system, we surveyed several kinds of photo-activable caged reagents for sonodynamic therapy. As caged reagents, we employed 5-carboxymethoxy-2-nitrobenzy
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l(CMNB)-caged fluorescein, Dextran,((5-carboxyraethoxy-2-nitrobenzyl) oxy) carbonyl-caged-5-(and-6)-carboxy-Q-rhodamine, and CMNB-caged carboxy fluorescein. To access the cleavage of caged crosslinkage, the increase of fluorescence was determined after sonolysis or photolysis of drugs. After the sonolysis of the reagents at a concentration of 1 μM in PBS, the intensity of fluorescence of solution increased in time dependence . Activation of drugs within the cultured cells was assayed with flow cytometer(Cytomks FC 500, Beckman-Coulter USA). PLB-985 cells(human myelomonoblastic leukemia cells derived from B-cell) were used for this assay. The cells were incubated with 10 PA CMNB-caged fluorescein in PPS for 10 minutes at 37℃. Cytometric analysis of sonicated cells showed a partial activation of CMNB-caged fluorescein within the cells. These results suggest the possibility of the device of new caged compounds using ultrasound, which are also expected as tracers and therapeutic reagents. Our system will open the possibility of new application of sonotherapy. Less
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Research Products
(57 results)
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[Journal Article] Effects of microbubbles on ultrasound-mediated gene transfer in human prostate cancer PC3 cells:Comparison among Levovist,YM454,and MRX-815H2008
Author(s)
Watanabe A, Otake R, Nozaki T, Morii A, Ogawa R, FUjimoto S, Nakamura S, Fuse H, Kondo T.
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Journal Title
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Identification of pH-sensitive regions in the mouse prion by the cysteine-scanning spin-labeling ESR technique.2006
Author(s)
Watanabe, Y., Inanami, O., Horiuchi, M., Hiraoka, W., Shimoyama, Y., Inagaki, F., Kuwabara, M.
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Journal Title
Biochemical and Biophysical Research Communications 350.3
Pages: 549-556
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Confirmation of enhanced expression of heme oxygenase-1 gene induced by ultrasound and its mechanism : Analysis by cDNA microarray system, real-time quantitative PCR, and weatern blotting.2006
Author(s)
Kagiya G., Tabuchi, Y., Feril, L. B. Jr., Ogawa, R., Zhao, Q.-L., Kudo, N., Hiraoda, W., Tachibana, K., Umemura, S., Kondo, T.
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Journal Title
Journal of Medical Ultrasonics 3.1
Pages: 3-10
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] An echo-contrast agent, Levovist, lowers the ultrasound intensity required to induce apoptosis of human leukemia cells.2006
Author(s)
Ando, H., Feril, L. B. Jr., Kondo, T., Tabuchi, Y., Ogawa, R., Zhao, Q.-L. Cui, Z. G., Umemura, S., Yoshikawa, H., Misaki, T.
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Journal Title
Cancer Letter 242.1
Pages: 37-45
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Expression of heme oxygenaes-1 due to intracellular reactive oxygen species induced by ultuasound.2006
Author(s)
Kagiya, G., Ogawa, R., Tabuchi, Y., Feril, L. B. Jr, Nozaki, T., Fukuda, S., Yamamoto, K., Kondo, T.
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Journal Title
Ultrasonics Sonochemistry 13.5
Pages: 388-396
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Effects of mictobubbles on ultrasound-mediated gene transfer in human prostatd cancer PC3 cells : Comparison among Levovist, YM454, and MRX-815H.
Author(s)
Watanabe A, Otade R, Nozaki T, Morii A, Ogawa R, Fujimoto S, Nakamura S, Fuse H, Kondo T.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Indentification of pH-sensitive regions in the mouse prion by the cysteine-scanning spin-labeling ESR technique.2006
Author(s)
Watanabe, Y., Inanami, O., Horiuchi, M., Hiraoka, W., Shimoyama, Y., Inagaki, f. Kuwabara, M.
Organizer
Biochemical and Biophysical Research Communications 350. 3
Year and Date
20060000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Confirmation of enhanced expression of heme oxygenase-1 gene induced by ultrasound and its mechanism : Analysis by cDNA microarray system, real-time quantitative Pcr, and western blotting.2006
Author(s)
Kagiya G. Tabuchi, Y., Feril, L. B. Jr., Ogawa, R., Zhao, Q. -L., Kudo, N., Hiraoka, W., Tachibana, K., Umemura, S., Kondo, T.
Organizer
Jorunal of Medical Ultrasonics 3. 1
Year and Date
20060000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] An echo-contrast agent, Levovist, lowers the ultrasound intensity required to induce apoptosis of human leukemia cells.2006
Author(s)
Ando, H., Feril, L. B. Jr., Kondo, T., Tabuchi, Y., Ogawa, R., Zhao, Q.-L, Cui, Z. G., Umemura, S., Yoshikawa, H., Misaki, T.
Organizer
Cancer Letter 242. 1
Year and Date
20060000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Expression of heme oxygenase-1 due to intracellular reavtive oxygen species induced by ultrasound.2006
Author(s)
Kagiya, G., Ogawa, R., Tabuchi, Y., Feril, L. B. Jr, Nozaki, T., Fukuda, S., Yamamoto, K., Kondo, T.
Organizer
Ultrasonics Sonochemistry 13. 5
Year and Date
20060000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Effects of microbubbles on ultrasound-mediated gene transfer in human prostate cancer PC3 cells : Comparison among Levovist, YM454, and MRX-815H.
Author(s)
Watanabe A, Otake R, Nozaki T, Morii A, Ogawa R, Fujimoto S, Nakamura S, Fuse H, Kondo T.
Organizer
Cancer Lett.
Description
「研究成果報告書概要(欧文)」より
