Development of Evaluation System for Coronary Vessel Regenerative Treatment Method Using 2- and 3-Dimensional X-ray Imaging

2008 Fiscal Year Final Research Report

• Project/Area Number: 18500359
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Japan Synchrotron Radiation Research Institute
• Principal Investigator: UMETANI Keiji Japan Synchrotron Radiation Research Institute, 利用研究促進部門・バイオ実験支援チーム, チームリーダー・主幹研究員 (50344396)
• Co-Investigator(Renkei-kenkyūsha): SHINOHARA Kunio 財団法人高輝度光科学研究センター, 利用研究促進部門, コーディネーター (10112088) ; SUGIMURA Kazuro 神戸大学大学院, 医学系研究科, 教授 (00136384)
• Project Period (FY): 2006 – 2008
• Keywords: 医用・生体画像

Research Abstract

本研究では、大型放射光施設SPring-8での放射光を利用し、2次元で血管血流の動画像を撮影する微小血管造影装置と、高解像度で広視野な3次元マイクロCT装置を改良し、生体における血管再生効果を視覚的及び定量的に評価する方法を確立した。これにより、ラットの心臓を使い2次元での血管血流の動画像で、血管再生での新規血管形成による心臓血管血流の改善を明らかにするなど、動態画像をもとにした機能解析を可能とした。さらに、心臓血管へバリウム造影剤を注入してホルマリン固定した標本を作製し、ラット心全体の3次元CT像を解像度6μmで撮影し、立体的に定量的評価を実施可能とした。

Research Products

• [Journal Article] Dilation of perforating arteries in rat brain in response to systemic hypotension is more sensitive and pronounced than that of pial arterioles -Simultaneous visualization of perforating and cortical vessels by invivo microangiography (2009)
  • Author(s): H. Yoshino, T. Sakurai, K. Umentani
  • Journal Title: Microvasc Res. 77(2) Pages: 230-233
  • Peer Reviewed
• [Journal Article] Contrast angiography of the rat renal microcirculation in vivo using synchrotron radiation (2009)
  • Author(s): G. A. Eppel, D. L. Jacono, K. Umetani
  • Journal Title: Am J Physiol Renal Physiol. 296(5) Pages: F1023-F1031
  • Peer Reviewed
• [Journal Article] 放射光マイクロCTでの腫瘍標本観察 (2009)
  • Author(s): 梅谷啓二
  • Journal Title: 画像ラボ 20(3) Pages: 36-40
• [Journal Article] Sensitive inspection of void defects using synchrotron radiation imaging with quantitative modeling of contrast enhancement (2009)
  • Author(s): K. Imamura, Y. Inada, K. Umetani
  • Journal Title: Insight 51(1) Pages: 12-15
  • Peer Reviewed
• [Journal Article] Synchrotron-based angiography for investigation of the regulation of vasomotor function in the microcirculation in vivo (2009)
  • Author(s): M. Shirai, D. O. Schwenke, K. Umetani
  • Journal Title: Clin. Exp. Pharmacol. Physiol. 36 Pages: 107-116
  • Peer Reviewed
• [Journal Article] Spectromicroscopic film dosimetry for high-energy microbeam from synchrotron radiation (2009)
  • Author(s): N. Nariyama, T. Ohigashi, K. Umetani, K. Shinohara
  • Journal Title: Appl. Radiat. Isot. 67 Pages: 155-159
  • Peer Reviewed
• [Journal Article] Dosimetry for a microbeam array generated by synchrotron radiation at SPring-8 (2008)
  • Author(s): M. Torikoshi, Y. Ohno, K. Umetani
  • Journal Title: Eur. J. Radiol. 688 (3) (Suppl. 1) Pages: S114-S117
  • Peer Reviewed
• [Journal Article] High-resolution visualization of tumours in rabbit lung using refraction contrast X-ray imaging (2008)
  • Author(s): T. Sera, K. Uesugi, K. Umetani
  • Journal Title: Eur. J. Radiol. 68 (3) (Suppl. 1) Pages: S54-S57
  • Peer Reviewed
• [Journal Article] Microangiography System for Investigation of Metabolic Syndrome in Rat Model Using Synchrotron Radiation (2008)
  • Author(s): K. Umetani, K. Fukushima, K. Sugimura
  • Journal Title: Proc. 30th Annual International IEEE EMBS Conference Pages: 2693-2696
  • Peer Reviewed
• [Journal Article] Dose distribution of a 125 keV mean energy microplanar x-ray beam for basic studies on microbeam radiotherapy (2008)
  • Author(s): Y. Ohno, M. Torikoshi, K. Umetani
  • Journal Title: Med. Phys. 35(7) Pages: 3252-3258
  • Peer Reviewed
• [Journal Article] Microcalcifications of Breast Tissue: Appearances on Synchrotron Radiation Imaging with 6-μm Resolution (2008)
  • Author(s): K. Imamura, N. Ehara, K. Umetani
  • Journal Title: Am. J. Roentgenol. 190 Pages: W234-W236
  • Peer Reviewed
• [Journal Article] Loss of CO2-induced Distensibility in Cerebral Arteries with Chronic Hypertension or Vasospasm after Subarachnoid Hemorrhage (2008)
  • Author(s): S. Nakajima, T. Kondoh, K. Umetani
  • Journal Title: Kobe J. Med. Sci. 53(6) Pages: 317-326
  • Peer Reviewed
• [Journal Article] In vivo microscopic X-ray imaging in rat and mouse using synchrotron radiation (2008)
  • Author(s): K. Umetani, T. Sakurai, T. Kondoh
  • Journal Title: SPIE 6816 Pages: 68160U_1-68160U_6
  • Peer Reviewed
• [Journal Article] Changes in macrovessel pulmonary blood flow distribution following chronic hypoxia - assessed using synchrotron radiation microangiography (2008)
  • Author(s): D. O. Schwenke, J. T. Pearson, K. Umetani
  • Journal Title: J Appl Physiol. 104 Pages: 88-96
  • Peer Reviewed
• [Journal Article] In Vivo Cerebral Artery Microangiography in Rat and Mouse Using Synchrotron Radiation Imaging System (2007)
  • Author(s): K. Umetani, K. Kidoguchi, A. Morishita
  • Journal Title: 29th Annual International IEEE EMBS Conference Pages: 3926-3929
  • Peer Reviewed
• [Journal Article] Simultaneous observation of superficial cortical and intracerebral microvessels in vivo during reperfusion after transient forebrain ischemia in rats using synchrotron radiation (2007)
  • Author(s): M. Morita, M. Ohkawa, K. Umetani
  • Journal Title: Brain Res. 1158 Pages: 116-122
  • Peer Reviewed
• [Journal Article] Synchrotron radiation coronary microangiography for morphometric and physiological evaluation of myocardial neovascularization induced by endothelial progenitor cell transplantation (2007)
  • Author(s): H. Iwasaki, K. Fukushima, K. Umetani, K. Sugimura
  • Journal Title: Arterioscler. Thromb. Vasc. Biol. 27(6) Pages: 1326-1333
  • Peer Reviewed
• [Journal Article] Visualization of intracerebral arteries by synchrotron radiation microangiography (2007)
  • Author(s): K. Myojin, A. Taguchi, K. Umetani
  • Journal Title: AJNR Am J Neuroradiol 28(5) Pages: 953-957
  • Peer Reviewed
• [Journal Article] Imaging of the pulmonary circulation in the closed-chest rat using synchrotron radiation microangiography (2007)
  • Author(s): D. O. Schwenke, J. T. Pearson, K. Umetani
  • Journal Title: J Appl Physiol 102(2) Pages: 787-793
  • Peer Reviewed
• [Journal Article] Microscopic X-ray imaging system for biomedical applications using synchrotron radiation (2007)
  • Author(s): K. Umetani, K. Kobatake, A. Yamamoto
  • Journal Title: SPIE 6501 Pages: 650112_1- 650112_7
  • Peer Reviewed
• [Journal Article] Evaluation of Tris-acryl Gelatin Microsphere Embolization with Monochromatic X Rays: Comparison with Polyvinyl Alcohol Particles (2006)
  • Author(s): A. Yamamoto, S. Imai, K. Umetani
  • Journal Title: J. Vasc. Interv. Radiol. 17 Pages: 1797-1802
  • Peer Reviewed
• [Journal Article] Quantification of distension in rat cerebral perforating arteries (2006)
  • Author(s): A. Morishita, T. Kondoh, K. Umetani
  • Journal Title: NeuroReport 17 Pages: 1549-1553
  • Peer Reviewed
• [Journal Article] Carotid artery occlusion and collateral circulation in C57Black/6J mice detected by synchrotron radiation microangiography (2006)
  • Author(s): M. Tamaki, K. Kidoguchi, K. Umetani
  • Journal Title: Kobe J. Med. Sci. 52(5) Pages: 111-118
  • Peer Reviewed
• [Journal Article] In Vivo X-Ray Angiography in the Mouse Brain Using Synchrotron Radiatio (2006)
  • Author(s): K. Kidoguchi, M. Tamaki, K. Umetani
  • Journal Title: Stroke 37(7) Pages: 1856-1861
  • Peer Reviewed
• [Journal Article] Impaired Response of Perforating Arteries to Hypercapnia in Chronic Hyperglycemia (2006)
  • Author(s): X. S. Oizumi, T. Akisaki, K. Umetani
  • Journal Title: Kobe J. Med. Sci. 52(2) Pages: 27-35
  • Peer Reviewed
• [Presentation] Development of Microangiographic Systems for Visualization, Quantification and Therapeutic Evaluation of Angiogenic Vessels (2009)
  • Author(s): T. Fujii, N. Fukuyama Y. Ikeya, E. Tanaka, T. Sekka, Y. Shinozaki, E.Sato, K. Fukushima, K. Hyodo, K. Umetani, K. Yamada, T. Tanabe, H. Mori
  • Organizer: 11th International Symposium on Anti-Angiogenic Agents: Recent Advances and Future Directions in Basic and Clinical Cancer Research
  • Place of Presentation: Hyatt Regency LaJolla, San Diego
  • Year and Date: 20090200
• [Presentation] Bystander effects in microplanar beam irradiated glioma cells (2008)
  • Author(s): G. Kashino, T. Kondo, N. Nariyama, T. Ohhigashi, K. Umetani, K. Shinohara, A. Kurihara, M. Fukumoto, H. Tanaka, A. Maruhashi, M. Suzuki, Y. Kinashi, S. Masunaga, M. Watanabe, K. Ono
  • Organizer: Workshop on New prospects for brain tumour radiotherapy: Synchrotron light and Microbeam Radiation Therapy
  • Place of Presentation: The European Synchrotron Radiation Facility, Grenoble
  • Year and Date: 20080600
• [Presentation] Dose distribution measurements of microbeam X rays using a spectromicroscopic method with GafChromic film (2008)
  • Author(s): N. Nariyama, T. Ohhigashi, K. Umetani, K. Shinohara, H. Tanaka, G. Kashino, A. Maruhashi, T. Kondo, A. Kurihara, M. Fukumoto
  • Organizer: Workshop on New prospects for brain tumour radiotherapy: Synchrotron light and Microbeam Radiation Therapy
  • Place of Presentation: The European Synchrotron Radiation Facility, Grenoble
  • Year and Date: 20080600
• [Presentation] Cross-planar microbeam irradiation can improve survival rate of rats with C6 glioma (2008)
  • Author(s): T. Kondoh, A. Katoh, E. Kohmura, A. Kurihara, M. Fukumoto, N. Nariyama, T. Ohhigashi, K. Umetani, K. Shinohara, G. Kashino, H. Tanaka, A. Maruhashi, K. Ono
  • Organizer: Workshop on New prospects for brain tumour radiotherapy: Synchrotron light and Microbeam Radiation Therapy
  • Place of Presentation: The European Synchrotron Radiation Facility, Grenoble
  • Year and Date: 20080600
• [Presentation] Dosimetry for a microbeam array generated by synchrotron radiation at SPring-8 (2007)
  • Author(s): M. Torikoshi, Y. Ohnoa, N. Yagi, K. Umetani, Y. Furusawa
  • Organizer: the Medical Applications of Synchrotron Radiation workshop
  • Place of Presentation: the University of Saskatchewan, Saskatoon
  • Year and Date: 20070800
• [Presentation] High-resolution visualization of tumours in rabbit lung using refraction contrast X-ray imaging (2007)
  • Author(s): T. Sera, K. Uesugi, N. Yagi, K. Umetani, M. Kobatake, S. Imai
  • Organizer: the Medical Applications of Synchrotron Radiation workshop
  • Place of Presentation: the University of Saskatchewan, Saskatoon
  • Year and Date: 20070800
• [Presentation] Microangiographic Imaging of Tumor Angiogenic Blood Vessels in Rabbit Model of Cancer Using High-Resolution, Real-Time Imaging System (2006)
  • Author(s): K. Umetani, M. Kobatake, A. Yamamoto, T. Yamashita, S. Imai
  • Organizer: the 9th International Conference on Synchrotron Radiation Instrumentation SRI2006
  • Place of Presentation: Exco Center, Daegu, South Korea
  • Year and Date: 20060500
• [Remarks] ホームページ等
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