2007 Fiscal Year Final Research Report Summary
Biomaterials combined with Engineered Growth Factors with collagen binding affinity for tissue regeneration.
| Project/Area Number |
18500371
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | The Institute of Physical and Chemical Research (2007)
Kanagawa Academy of Science and Technology (2006) |
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Principal Investigator |
KITAJIMA Takashi The Institute of Physical and Chemical Research, Nano Medical Engineering Laboratory, Contract Researcher (40399556)
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| Co-Investigator(Kenkyū-buntansha) |
ITO Yoshihiro RIKEN, Nano Medical Engineering Laboratory, Chief Scientist (40192497)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Regenerative Medicine / Tissue Engineering / Growth Factors / Blood Vessels |
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| Research Abstract |
We engineered growth factors (EGF, HGF, VEGF etc) to confer collagen binding activity by fusion of fibronectin collagen-binding domain (CBD). Resulting engineered growth factors showed stable binding activity. Cell growth promotion activity was also improved when they were combined with collagen. One of such fusion protein (CBD-HGF) immobilized to the culture vessels continued to stimulate growth of endothelial cells without medium change. That is, additional factor was unnecessary during prolonged culture. This will be suitable for developing culture system (or bioreactor) of growing large number of cells for cell-implantation therapy.
We further examined CBD-HGF on the applicability to regenerative medicine by combining with tissue engineering materials as follows.
1) Small caliber artificial blood vessels (3mm in diameter, ePTFE) were combined with CBD-HGF and implanted into dog femoral artery. The implanted vessels showed earlier endothelialization (4weeks) as compared to control vessels and native HGF treated vessels. The fusion HGF may be used to develop implantable vessels with long patency for bypass graft of coronary artery.
2) Collagen sponges combined with CBD-HGF induced extensive angiogenesis by 2 weeks after implantation into rat subcutaneous tissue, while control and HGF-treated sponge showed minimal angiogenesis. The sponge may be useful to repair ischemic cardiac tissues, leg ulcer etc.
3) CBD-HGF infused to balloon-injured carotid artery of rats promoted endothelialization of denuded areas. It may be used to prevent restenosis after angioplasty using balloon catheter.
The results of the present study indicated the applicability of collagen-binding growth factor (s) in the following ways;
1) Culture system for cell therapy, 2) biomaterial combined with growth factor activity to induce tissue repair and 3) medicine to induce tissue repair.
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[Presentation] 組織工学材料への増殖因子固定化2006
Author(s)
北嶋 隆、伊藤 嘉浩
Organizer
日本バイオマテリアル学会大会(第8回)
Place of Presentation
東京(アルカディア市ケ谷)
Year and Date
2006-11-27
Description
「研究成果報告書概要(和文)」より
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