2007 Fiscal Year Final Research Report Summary
Studies on the adipocyte differentiation and the effects of food factors
| Project/Area Number |
18500621
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Eating habits, studies on eating habits
|
|---|
| Research Institution | Ochanomizu University |
|---|
Principal Investigator |
FUJIWARA Yoko Ochanomizu University, Graduate School of Humanities and Sciences, Food and Nutritional Science, Associate professor (50293105)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Keywords | adipocyte / DNA microarray / adipocytokine / polyunsaturated fatty acid / inflammation |
|---|
| Research Abstract |
Adipocytes differentiation and the effects of food factors were studied. We used the SGBS (Simpson-Golabi-Behmel Syndrome) cell line as the model of human adipocytes and also analyzed the expression profiles of SGBS cells treated by polyunsaturated fatty acid (PUFA), like arachidonic acid (AA) and eicosapentaenoic acid (EPA), using a DNA microarray and pathway analysis. Although EPA was reported to inhibit the differentiation of pre-adipocytes to mature adipocyte, we hind that both AA and EPA did not effect the differentiation of adipocytes. Unexpectedly, not only AA but also EPA strongly upregulated the expression of genes related to the inflammation, such as IL6, IL8, NFKB. Addition of antioxidant or inhibitors for production of inflammatory prostagrandin did not fully block the elevation of expression of IL6 by both PUFAs. We screened some food factors to diminish the induction of IL6 by AA in adipocytes, and found that α-tocoopherol, γ-tocotrienol, and resveratrol were effective.
|
Research Products
(14 results)
-
-
-
-
[Presentation] Effects of resveratrol and its analogues on adipocytes2008
Author(s)
M, Hasebe, M, Iwamura, K, Fukuhara, A, Matsuoka, S, Ikemoto, Y, Fujiwara
Organizer
Annual meeting of Japan Society for Bioscience and Biotechnology and Agrochemistry
Place of Presentation
Nagoya, Japan
Year and Date
2008-03-27
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
[Presentation] DNA microarray analysis of the SGBS adipocytes treated by arachidonic and eicosapentaenoic acids2006
Author(s)
M, Iwamura, I, Kurihara, T, Ando, P, Fischer-Posovszky, M, Wabitsch, Y, Otsuka, S, Ikemoto, Y, Fujiwara
Organizer
2006 annual forum of Japanese Molecular Biology
Place of Presentation
Nagoya Japan
Year and Date
2006-12-08
Description
「研究成果報告書概要(欧文)」より
-
[Presentation] Netwark analysis of transcriptional gene expressions of adipocytes and effect of polyunsaturated fatty acids2006
Author(s)
I, Kurihara, M, Iwamura, T, Ando, P, Fischer-Posovszky, M, Wabitsch, Y, Otsuka, S, Ikemoto, Y, Fujiwara
Organizer
2006 annual forum of Japanese Molecular Biology
Place of Presentation
Nagoya Japan
Year and Date
2006-12-08
Description
「研究成果報告書概要(欧文)」より
-
-
