• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2007 Fiscal Year Final Research Report Summary

EVALUATION OF FOODS ALLERGY USING A VOLUNTARY PHYSICAL ACTIVITY IN MICE, AND ITS APPLICATION AS EVALUATION OF PROCESSED FOODS

Research Project

Project/Area Number 18500634
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Eating habits, studies on eating habits
Research InstitutionKawasaki University of Medical Welfare

Principal Investigator

YANO Hiromi  Kawasaki University of Medical Welfare, FACULTY OF HEALTH SCIENCE AND TECHNOLOGY, ASSOCIATE PROFESSOR (20248272)

Project Period (FY) 2006 – 2007
KeywordsFood allergy / Anaphylaxis / Voluntary physical activity / Histamine / Leukotriene / Platelet-activation factor / Lysozyme / Ovalbumin
Research Abstract

In this study, we evaluated whether the voluntary physical activity, i.e., the revolution of a running wheel, is a novel and unique indication for the evaluation of the active systemic anaphylaxis in a mouse model or not. B10.A mice were immunized with egg lysozyme (LYZ) to induce specific IgE response, subsequently received an i.v. injection of the antigen, and then subjected to the body temperature measurement followed by the counting of the running wheel revolution. The injection with antigen inhibited in both the rectal temperature and the voluntary activity significantly reduced. The injection with histamine or leukotriene, which have been thought the main chemical mediators of anaphylaxis, significantly reduced the rectal temperature, but did not affect the wheel-running activity. In fact, although a high correlation between the total wheel-revolution and the lowering of body temperature caused by the antigen injection was observed (r=0.812, p<0.01), neither histamine nor leukotriene treated mice was shown the significant correlation.
Furthermore, we determined the role of mast cells, which are the main cells of histamine and leukotriene production, on anaphylaxis in mice using mast cells deficient mice. As the results, although mast cells deficient mice also induced anaphylaxis after antigen re-exposure, the anaphylactic reactions were prevented by platelet-activating factor (PAF) receptor antagonist.
These results suggest that the voluntary physical activity is a novel indication for the analyses of hypersensitivity reactions caused by LYZ re-exposure in the sensitized mice. In addition the reduction of physical activity in the sensitized mice might not be caused by must cells induced histamine and leukotriene.

  • Research Products

    (8 results)

All 2008 2007

All Journal Article (6 results) (of which Peer Reviewed: 3 results) Presentation (2 results)

  • [Journal Article] The reduction of voluntary physical activity after poly I:C injection is independent of the effect of poly I:C-induced interferon-beta in mice2008

    • Author(s)
      Matsumoto T, Takahashi H, Shiva D, Kawanishi N, Kremenik MJ, Kato Y, Yano H.
    • Journal Title

      Physiol.Behav. 93

      Pages: 835-841

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Lipopolysaccharide-induced monocyte chemotactic protein-1 is enhanced by suppression of nitric oxide production,which depends on poor CD14 expression on the surface of skeletal muscle2008

    • Author(s)
      Kawanishi N, Tanaka Y, Kato Y, Shiva D, Yano H.
    • Journal Title

      Cell Biochem.Funct. 26

      Pages: 486-492

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] High dose of lipopolysaccharide pre-treatment prevents OVA-induced anaphylactic decreases in rectal temperature in the immunized mice2008

    • Author(s)
      Shiva D, Matsumoto T, Kremenik MJ, Kato Y, Yano H.
    • Journal Title

      Immunol.Lett. 118

      Pages: 59-64

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] The reduction of voluntary physical activity after poly I : C injection is independent of the effect of poly IC-induced interferon-beta in mice.2008

    • Author(s)
      Matsumoto T, Takahashi H, Shiva D, Kawanishi N, Kremenik MJ, Kato Y, Yano H.
    • Journal Title

      Physiol. Behay. 93 (4)

      Pages: 835-841

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Lipopolysaccharide-induced monocyte chemotactic protein-1 is enhanced by suppression of nitric oxide production, which depends on poor CD14 expression on the surface of skeletal muscle.2008

    • Author(s)
      Kawanishi N, Tanaka Y, Kato Y,Shiva D, Yano H.
    • Journal Title

      Cell Biochem. Funct. 26(4)

      Pages: 486-492

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] High dose of lipopolysaccharide pre-treatment prevents OVA-induced anaphylactic decreases in rectal temperature in the immunized mice.2008

    • Author(s)
      Shiva D, Matsumoto T, Kremenik MJ, Kato Y, Yano H.
    • Journal Title

      Immunol. Lett. 118(1)

      Pages: 59-64

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Why wheat-dependent exercise-induced anaphylaxis is provoked by various wheat processed foods2007

    • Author(s)
      Tanaka M, Yano H, Kato Y.
    • Organizer
      The 8th International Society of Exercise Immunology Symposium
    • Place of Presentation
      Sendai,Japan
    • Year and Date
      20071025-27
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Why wheat-dependent exercise-induced anaphylaxis is provoked by various wheat processed foods.2007

    • Author(s)
      Tanaka M, Yano H, Kato Y
    • Organizer
      The 8th International Society of Exercise Immunology Symposium
    • Place of Presentation
      Sendai, Japan
    • Year and Date
      20071025-27
    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2010-02-04  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi