2007 Fiscal Year Final Research Report Summary
The study of modulating effects on X-ray induced cell mutability by chaperons and autophagy
| Project/Area Number |
18510041
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Chiba University |
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Principal Investigator |
SUZUKI Nobuo Chiba University, Graduate School of Medicine, PROFESSOR (90111426)
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| Co-Investigator(Kenkyū-buntansha) |
KITA Kazuko Chiba University, Graduate School of Medicine, ASSOCIATE PROFESSOR (80302545)
SUZUKI Toshikazu Chiba University, Graduate School of Medicine, RESEARCH ASSOCIATE (70270527)
SUGAYA Shigeru Chiba University, Graduate School of Medicine, RESEARCH ASSOCIATE (90334177)
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| Project Period (FY) |
2006 – 2007
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| Keywords | annexin II / aldolase A / human serum / chaperon / autophagy / X-ray / human cell / UV (ultraviolet) |
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| Research Abstract |
The present work is designed to investigate the role of chaperons and autophagy on the suppressive physiological functions for radiation-induced mutagenicity in human. (1) We previously reported that HSP27 is involved in cellular DNA-repair functions after UV irradiation. In the present study, annexinII was identified as a candidate gene for the HSP27 interacting protein, and suggested to be involved in cellular UV resistance by the colony forming assay using siRNA for annexinII mRNA. (2) We also tried proteomic studies for elucidation of radiation responsible genes. Using X-ray irradiated-nuclear cell extracts, we found some candidate genes. Aldolase A, one of genes for glycolytic enzyme proteins, was suggested to be involved in cellular X-ray resistance. (3) To moreover examine the involvement of autophagy in radiation response, expression levels of LC3, one of autophagy marker proteins, were analyzed using HSP27-suppressive cell lines. We could not find any difference of LC3 expression levels between HSP27-suppressive and control cells, although increased levels of LC3 was observed after serum starvation. (4) In human body oxidative stress conditions are expected to play roles on modulation of radiation response. Therefore, the test to monitor the oxidative stress by evaluating serum hydroperoxide was carried out. Amounts of serum hydroperoxide showed higher levels in patients, such as rheumatoid arthritis and diabetes mellitus, than healthy volunteers. Thus, it will be an intriguing problem whether radiation susceptibility in human is regulated by combination of chaperones metabolism with oxidative conditions.
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Research Products
(42 results)
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[Journal Article] Life Science of Gravity-changing Stress; Modulation of UV-mutagenicity in human RSb Cells by Serum Factors from Volunteers Who Experienced Parabolic Flight.2006
Author(s)
Suzuki, N., Chi, X., Takahashi, S., Nomura, J., Sugaya, S., Ichimura, Y., Zhai, L., Tong, X., Kita, K.
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Journal Title
J. Intl. Soc. Life Info. Sci. 24
Pages: 362-363
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Life Science of Gravity-changing Stress; Modulation of UV-mutagenicity in human RSb Cells by Serum Factors from Volunteers Who Experienced Parabolic Flight2006
Author(s)
Suzuki, N., Chi, X., Takahashi, S., Nomura, J., Sugaya, S., Ichimura, Y., Zhai, L., Tong, X., Kita, K.
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Journal Title
J. Intl. Soc. Life Info. Sci 24(2)
Pages: 362-363
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Annexin II, a novel HSP27-interacted protein, is involved in resistance to UVC-induced cell death in human AP^r-1 cells.
Author(s)
Tong, X., Kita, K., Karata, K., Zhu, C., Sugaya, S., Ichimura Y., Satoh, M., Tomonaga, T., Nomura, F., Jin, Y., Suzuki, N.
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Journal Title
Photochem. Photobiol (In press)
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Involvement of aldolase A in X-ray resistance of human HeLa and UV^r-1 cells
Author(s)
J., Lu, T., Suzuki, M., Satoh, S., Chen, T., Tomonaga, F., Nomura, N., Suzuki
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Journal Title
Biochemical and Biophysical Research Communications 369
Pages: 948-952
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Annexin II a novel HSP27-interacted protein, is involved in resistance to UVC-induced cell death in human AP^r-1 cells
Author(s)
Tong, X., Kita, K., Karata, K., Zhu, C., Sugaya, S., Ichimura Y, Satoh, M., Tomonaga, T., Nomura, F., Jin, Y, Suzuki, N.
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Journal Title
Photochem. Photobiol (in press)
Description
「研究成果報告書概要(欧文)」より
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[Presentation] HSP27and its interacted protein, is involved in susceptibility to radiation lethality in human cells.2007
Author(s)
Tong Xiao bo, Kita K., Karata K., Zhu C., Sugaya S., Ichimura Y., Sato M., Tomonaga T., Nomura F., Jin Y-H., Suzuki N.
Organizer
Keystone Symposia on Molecular and cellular Biology, Gastointestinal Cancer (T2)
Place of Presentation
Beijing, China
Year and Date
2007-10-17
Description
「研究成果報告書概要(和文)」より
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[Presentation] HSP27 and its interacted protein, is involved in susceptibility to radiation lethality in human cells2007
Author(s)
Tong Xiao bo, Kita K., Karata K, Zhu C., Sugaya S., Ichimura Y., Sato M., Tomonaga T., Nomura F., Jin Y-H,. Suzuki N.
Organizer
Keystone Symposia on Molecular and cellular Biology, Gastointestinal Cancer (T2)
Place of Presentation
Beijing, China
Year and Date
2007-10-17
Description
「研究成果報告書概要(欧文)」より
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