Prediction of the cytotoxicity of additives in cosmetics on UV-exposed human skin keratinocytes

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 18510062
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: HANDA Osamu Kyoto Prefectural University of Medicine, Department of Biomedical Safety Science, Assistant Professor (90381970)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Toshikazu Kyoto Prefectural University of Medicine, Department of Inflammation and Immunology, Professor (90158410) ; YOSHIDA Norimasa Kyoto Prefectural University of Medicine, Department of Molecular Gastroenterology and Hepatology, Assistant Professor (30166962) ; NAITO Yuji Kyoto Prefectural University of Medicine, Department of Medical Proteomics, Associate Professor (00305575) ; KOKURA Satoshi Kyoto Prefectural University of Medicine, Department of Biomedical Safety Science, Associate Professor (80347442)
• Project Period (FY): 2006 – 2007
• Keywords: cosmetic additives / toxicology / ultra violet / genomic analysis / keratinocvte

Research Abstract

For many years, methylparaben (MP) has been used as a preservative in cosmetics. In this study, we investigated the effects of ultraviolet-B(UVB)exposure on MP-treated human skin keratinocytes. HaCaT keratinocyte was cultured in MP-containing medium for 24 h, exposed to UVB (15 or 30 mJ/cm2) and further cultured for another 24 h. Subsequent cellular viability was quantified by MTT-based assay and cell death was qualified by fluorescent microscopy and flow cytometry. Oxidative stress, nitric oxide (NO) production and cellular lipid peroxidation were measured using fluorescent probes. In addition, activation of nuclear factor kappa B and activator protein-1 was assessed by electro-mobility gel-shift assay. Practical concentrations of MP (0.003%) had a little or no effect on cellular viability, oxidative stress, NO production, lipid peroxidation and activation of nuclear transcription factors in HaCaT keratinocytes. Low-dose UVB also had little or no effect on these parameters in HaCaT keratinocytes. However, UVB exposure significantly increased cell death, oxidative stress, NO production, lipid peroxidation and activation of transcription factors in MP-treated HaCaT keratinocytes. These results indicate that MP, which has been considered a safe preservative in cosmetics, may have harmful effects on human skin when exposed to sunlight.

Research Products

• [Journal Article] Methylparaben potentiates UV-induced damage of skin keratinocytes (2006)
  • Author(s): 半田 修、古倉 聡、足立 聡子、高木 智久、内藤 裕二、谷川 徹、吉田 憲正、吉川 敏一
  • Journal Title: Toxicology 227巻 Pages: 62-72
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Methylparaben potentiates UV-induced damage of skin keratinocytes (2006)
  • Author(s): Handa, O., Kokura, S., Adachi, S., Takagi, T., Naito, Y., Tanigawa, T., Yoshida, N., Yoshikawa, T
  • Journal Title: Toxicology 227(1-2) Pages: 62-72
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] UV potentiates cytotoxicity of additives in cosmetics on human skin keratinocytes (2007)
  • Author(s): Osamu Handa
  • Organizer: 8th World Congresson Inflammation
  • Place of Presentation: Copenhagen,Denmark.
  • Year and Date: 2007-06-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] UV potentiates cytotoxicity of additives in cosmetics on human skin keratinocytes (2007)
  • Author(s): Osamu, Handa, Satoshi, Kokura, Katsura, Mizushima, Satomi, Akagiri, Tomohisa, Takagi, Yuji, Naito, Norimasa, Yoshida, Toshikazu, Yoshikawa
  • Organizer: 8th World Congress on Inflammation
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2007-06-19
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ビタミンEによるメチルパラベン/紫外線惹起性ケラチノサイト細胞毒性軽減 (2007)
  • Author(s): 半田 修
  • Organizer: 第18回ビタミンE研究会
  • Place of Presentation: 長崎
  • Year and Date: 2007-01-26
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Vitamin E ameliorates methylparaben/ultraviolet-induced cytotoxicity on skin keratinocytes (2007)
  • Author(s): Osamu, Handa, Satoshi, Kokura, Satomi, Akagiri, Tomohisa, Takagi, Yuji, Naito, Norimasa, Yoshida, Toshikazu, Yoshikawa
  • Organizer: 18th annual meeting of Japanese society of vitamin E research
  • Place of Presentation: Nagasaki, Japan
  • Year and Date: 2007-01-26
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 紫外線照射下での化粧品添加物の皮膚ケラチノサイトに対する毒性発現の検討 (2006)
  • Author(s): 半田 修
  • Organizer: 日本過酸化脂質・フリーラジカル学会 第30回大会
  • Place of Presentation: 東京
  • Year and Date: 2006-10-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Cytotoxic effect of cosmetic additives on ultraviolet exposed Skin keratinocytes (2006)
  • Author(s): Osamu, Handa, Satoshi, Kokura, Katsura, Mizushima, Tomohisa, Takagi, Hiroshi, Ichikawa, Yuji, Naito, Toru, Tanigawa, Norimasa, Yoshida, Toshikazu, Yoshikawa
  • Organizer: 30th annual meeting of Japanese Society for Lipid peroxidation and Free Radical Research
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2006-10-20
  • Description: 「研究成果報告書概要(欧文)」より