2007 Fiscal Year Final Research Report Summary
Identification and topology analysis of plant odorant receptors
| Project/Area Number |
18510186
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Hiroshima University |
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Principal Investigator |
IZUMI Shunsuke Hiroshima University, Graduate School of Science, Associate Professor (90203116)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Odorant receptors / Proteomics / Biotransformation / Membrane topology / Chemical modification / Mass spectrometry |
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| Research Abstract |
Volatile substances and their receptors was involved in plant-plant communications.
We have discussed the roles of terpenes, green odor, Me jasmonate, and plant GPCR (G protein-coupled receptors), two-component system, and chemoreceptor channels as candidates for plant odorant receptors.
Next, Protein clusters rule the stereoselectivity of pulegone reductase; the pulegone reductase which bound with β-glucosidase and trypsin inhibitor catalyzed (1R,4R)-isomenthone preferentially rather than (1R,4S)-menthone from (R)-pulegone.
Furthermore, the topology and the dynamics of peptide within the liposomes were investigated by use of mass spectrometry combined with acetylation. The peptide within theliposomes was acetylated with NHSSAc; the N-terminal of melittin was acetylated. Thus, it was supposed that the peptide within the liposomes adopted an N-terminal topology.
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Research Products
(8 results)
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[Journal Article] Nucleotide excision repair and homologous recombination systems commit differentially to the repair of DNA-protein crosslinks.2007
Author(s)
Nakano, Toshiaki, Morishlta, Soh, Katafuchi, Atsushi, Matsubara, Mayumi, Horikawa, Yusuke, Terato, Hiroak, Salem, Amir M. H., Izumi, Shunsuke, Pack, Seung Pil, Makino, Keisuke, Ide, Hiroshi.
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Journal Title
Molecular Cell 28(1)
Pages: 147-158
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Nucleotide excision repair and homologous recombination systems commit differentially to the repair of DNA-protein crosslinks.2007
Author(s)
Nakano, Toshiaki, Morishita, Soh, Katafuchi, Atsushi, Matsubara, Mayumi, Horikawa, Yusuke, Terato, Hiroaki, Salem, Amir M. H., Izumi, Shunsuke, Pack, Seung Pil, Makino, Keisuke, Ide, Hiroshi
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Journal Title
Molecular Cell 28
Pages: 147-158
Description
「研究成果報告書概要(欧文)」より
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