2007 Fiscal Year Final Research Report Summary
Development and application of assay systems for searching antipruritic drug
| Project/Area Number |
18510196
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Mukogawa Women's University |
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Principal Investigator |
OKU Hisae Mukogawa Women's University, School of Pharmacy and Pharmaceutical Sciences, Assistant (90281518)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIGURO Kyoko Mukogawa Women's University, School of Pharmacy and Pharmaceutical Sciences, Professor (70151363)
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| Project Period (FY) |
2006 – 2007
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| Keywords | bioactive / Pharmacy / animal model of disease / itch / Natural resources / stress |
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| Research Abstract |
The following report describes our research using the mouse pruritis-model to investigate the factors that trigger pruritis and contribute to its worsening. Moreover, we also extended our research to anti-pruritics as an effort to discover new itch-suppression materials from natural resources which are essential in treatment of atopic dermatitis and other skin inflammations.
1. Central nervous system mechanisms present in the model for transient pruritis have been observed in the NC mice of atopic dermatitis model. This model was used for atopic dermatitis in an effort to create a model that exhibits worsening of pruritis in response to stress. In order to quantify the stress level in mice, biochemistry of blood was performed to measure the concentrations of corticosterone, a stress hormone. Then, using a communications box purchased out of the equipment budget for this application, we applied random direct shocks to induce stress in mice, creating easily replicable stress conditions within a short span of time. Consequently, we also investigated the correlation and relationship between the degree of stress and the scratching behaviour in vivo.
2. We used a variety of assay methods to create an index for measuring the frequency of scratching for degranulating compound 48/80, serotonin and platelet activating factor, and protease, which specifically increase at the site of inflammation. In our search for seeds from natural resources, containing anti-pruritic properties we discovered that the bud of the white Rose of Sharon, obtained from the Shrubalthea Flower, Chinese medicine was effective, we investigated the effectiveness of different varieties of Rose of Sharon, and observed that the red buds were more effective against allergic pruritis than the white buds used in Chinese medicine. Moreover, we were also able to clarify that the active ingredients consisted of several varieties of flavonol and anthocyan derivatives.
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[Journal Article] Primary preventive teas on metabolic syndrome-mechanisms of Kinginkacha and Kinkacha-2007
Author(s)
Oku, H., Ogawa, Y., Iwaoka, E., Yamaguchi, Y., Kunitomo, M. and Ishiguro, K.
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Journal Title
Yakugaku Zasshi 127
Pages: 40-42
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Primary preventive teas on metabolic syndrome-mechanisms of Kinginkacha and Kinkacha-2007
Author(s)
Oku, H., Ogawa, Y., Iwaoka, E., Yamaguchi, Y., Kunitomo, M., Ishiguro, K.
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Journal Title
Yakugaku Zasshi 127
Pages: 40-42
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Garcinone B reduces prostaglandin E2 release and NF-kB-mediated transcription in C6 rat glioma cells2006
Author(s)
Yamakuni, T., Aoki, K., Nakatani, K., Nondo, N., Oku, H., Ishiguro, K. and Ohizumi, Y.
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Journal Title
Neuroscience Letters 394
Pages: 206-210
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Garcinone B reduces prostaglandin E2 release and NF-kB-mediated transcription in C6 rat glioma cells.2006
Author(s)
Yamakuni, T., Aoki, K., Nakatani, K., Nondo, N., Oku, H., Ishiguro, K., Ohizumi, Y.
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Journal Title
Neuroscience Letters 394
Pages: 206-210
Description
「研究成果報告書概要(欧文)」より
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