2007 Fiscal Year Final Research Report Summary
Development for the method of minute plant cell analysis by fluorescence. correlation spectroscopy.
| Project/Area Number |
18550080
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
FUJIWARA Kitao Tokyo University of Pharmacy and Life Science, School of Life Sciences, Professor (90090521)
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| Co-Investigator(Kenkyū-buntansha) |
AOKI Motohide Tokyo University of Pharmacy and Life Sciences, School of Life Sciences, Assistant Professor (30418917)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Fluorescence spectroscopy / Micro-algae / Chlorophyl / Correlation Spectroscopy / Nano-particle / Particle size determination / プランクトン / 大腸菌 |
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| Research Abstract |
Fluorescence correlation spectrometry (FCS) is the novel technique which can determine the size of fluorescent particles. The optical parameters such as diameter of pin-hole aperture and laser beam size were inspected in the response of FCS signal against the variation in the diameters of fluorescent particles. In the ordinary system, con-focal optical configuration was adopted. However, it has been proved that the FCS system with the ordinary optical configuration of fluorescence spectroscopy can determine the size lass than 100 nm. The present system with the ordinary optical fluorescence configuration can successfully determine the mico-algae.
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