Development of threshold-energy dissociation mass spectrometry for characterization of biomolecules

2008 Fiscal Year Final Research Report

• Project/Area Number: 18550085
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: NAKAMURA Takemichi The Institute of Physical and Chemical Research, 物質構造解析チーム, 先任研究員 (10360611)
• Project Period (FY): 2006 – 2008
• Keywords: 質量分析法 / 閾値エネルギー解離 / 生体分子 / イオントラップ / 解離反応経路 / 選択的断片化 / 衝突冷却 / 有機化合物定性

Research Abstract

研究成果の概要:低分子有機化合物の定性および定量分析法として質量分析法は最も高感度かつ特異性の高い方法の一つであるが,生体分子の多くに対しては電子イオン化法を適用できず,化合物の同定は非常に困難な作業となる.閾値エネルギー解離法に関する本研究の結果,汎用性が高く小型化可能なイオントラップ質量分析計を用いて,本法を生体分子を含む広範な低分子有機化合物の定性・同定法へと展開していくための基盤を構築できた.

Research Products

• [Journal Article] マススペクトルの解析と有機化合物の定性 - 質量分析実務者のための基礎講座 (2009)
  • Author(s): 中村健道
  • Journal Title: Journal of the Mass Spectrometry Society of Japan 57(印刷中) Pages: 0
  • Peer Reviewed
• [Journal Article] Factors governing peptide backbone cleavages in electron capture dissociation of triantennary complex-type N-glycosylated peptide (2007)
  • Author(s): Hongo Y, , Sato A, Nakamura T.
  • Journal Title: Journal of the Mass Spectrometry Society of Japan 55 Pages: 279-285
  • Peer Reviewed
• [Journal Article] Electron capture dissociation of triantennary complex-type N-glycosylated peptides: a case of suppressed peptide backbone cleavage (2007)
  • Author(s): Hongo Y, Nakamura T, Sato A.
  • Journal Title: Journal of the Mass Spectrometry Society of Japan 55 Pages: 77-82
  • Peer Reviewed
• [Journal Article] Phosphatidylglucoside exists as a single molecular species with saturated fatty acyl chains in developing astroglial membranes (2006)
  • Author(s): Nagatsuka Y, Horibata Y, Yamazaki Y, Kinoshita M, Shinoda Y, Hashikawa T, Koshino H, Nakamura T, Hirabayashi Y.
  • Journal Title: Biochemistry 45 Pages: 8742-8750
  • Peer Reviewed
• [Presentation] スペクトル解析と定性 - MS とMS/MS を有機化学的視点から考える (2008)
  • Author(s): 中村健道
  • Organizer: 第35回BMSコンファレンス(BMS2008)
  • Place of Presentation: 裏磐梯ロイヤルホテル
  • Year and Date: 2008-07-08
• [Presentation] Inconvenient Reality: Is the "Hydrogen-shift" in Electron Capture Dissociation of Peptide Backbone Predictable? (2007)
  • Author(s): Takemichi Nakamura
  • Organizer: 55th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Indiana Convention Center in Indianapolis
  • Year and Date: 2007-06-04
• [Presentation] ECD スペクトルの解釈について - 有機マススペクトロメトリーの視点から考える (2007)
  • Author(s): 中村健道
  • Organizer: 第55回質量分析総合討論会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2007-05-16
• [Presentation] A proposal for extension of Biemann's nomenclature: peptide backbone fragmentation with odd electron species (2006)
  • Author(s): Takemichi Nakamura
  • Organizer: 22nd Asilomar Conference on Mass Spectrometry: Fundamentals of Gas Phase Ion Chemistry
  • Place of Presentation: Asilomar Conference Center
  • Year and Date: 2006-10-21