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2007 Fiscal Year Final Research Report Summary

High performance EQCM technique for analysis of ocidoreducetase

Research Project

Project/Area Number 18550149
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemistry related to living body
Research InstitutionNagoya University (2007)
Tokyo Institute of Technology (2006)

Principal Investigator

KAMACHI Toshiaki  Nagoya University, Department of Engineering, Associate Professor (30272694)

Project Period (FY) 2006 – 2007
KeywordsEQCM / Cytochrome Ca / ヒドロゲナーゼ
Research Abstract

Cytochrome c3 from Desulfovibrio vulgaris has four hemes per molecule, and a redox change at the hemes alters the conformation of the protein, leading to a redox-dependent change in the interaction of cytochrome c3 with redox partners (an electron acceptor or an electron donor) .The redox-dependent change in this interaction was directly monitored by the high-performance electrochemical quartz crystal microbalance (EQCM) technique that has been improved to give high sensitivity in solution. In this method, cytochrome c3 molecules in solution associate electrostatically with a viologen-immobilized quartz crystal electrode as a monolayer, and redox of the associating cytochrome c3 is controlled by the immobilized viologen. This technique makes it possible to measure the access of cytochrome c3 to the electrode or repulsion from the electrode, and hence interconversion between an electrostatic complex and an electron transfer complex on the cytochrome c3 and the viologen as a mass change accompanying a potential sweep is monitored. In addition, simultaneous measurement of a niece change and a potential step reveals that the cytochrome c3 stores electrons when the four hemes are reduced (an electron pool effect), that is, the oxidized cytochrome c3 facilitates acceptance of electrons from the immobilized viologen molecule, but the reduced cytochrome c3 donates the accepted electrons to the viologen with difficulty.

  • Research Products

    (23 results)

All 2008 2007 2006

All Journal Article (13 results) (of which Peer Reviewed: 3 results) Presentation (7 results) Book (3 results)

  • [Journal Article] Role of positive charge of lysine residue on cytochrome c3 for electrostatic interaction with hydrogenase2007

    • Author(s)
      Iida, S., Asakura, N., Tabata, K., Okura, I, and Kamachi, T.
    • Journal Title

      Jonrnal of Porphyrins and Phthalocyanines 11

      Pages: 66-73

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] N-terminus methylation of desulfovibrio vulgaris(miyazaki)Fcytochrome c32007

    • Author(s)
      Iida, S., Kawano, Y., Asakura, N., Tabata, K., Okura, I., and Kamachi, T.
    • Journal Title

      Chemistry Letters 36

      Pages: 184-185

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Oxygen concentration imaging in a single living cell using phosphorescence lifetime of Pt-porphyrin2007

    • Author(s)
      Saito, T., Asakura, N., Kamachi, T., and Okura, I.
    • Journal Title

      Journal of Porphyrins and Phthalocyanines 11

      Pages: 160-164

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] 「研究成果報告書概要(欧文)」より2007

    • Author(s)
      Iida, S., Asakura, N., Tabata, K., Okura, I., Kamachi, T
    • Journal Title

      Journal of Porphyrins and Phthalocyanines 11

      Pages: 66-73

  • [Journal Article] 「研究成果報告書概要(欧文)」より2007

    • Author(s)
      Iida, S., Kawano, Y., Asakura, N., Tabata, K., Okura, I., Kamachi, T
    • Journal Title

      Chemistry Letters 36

      Pages: 184-185

  • [Journal Article] 「研究成果報告書概要(欧文)」より2007

    • Author(s)
      Saito, T., Asakura, N., Kamachi, T., Okura, I
    • Journal Title

      Journal of Porphyrins and Phthalocyanines 11

      Pages: 160-164

  • [Journal Article] 「研究成果報告書概要(欧文)」より2006

    • Author(s)
      Asakura, N. Kamachi, T., Okura, I
    • Journal Title

      Research on Chemical Intermediates 32

      Pages: 341-355

  • [Journal Article] 「研究成果報告書概要(欧文)」より2006

    • Author(s)
      Asakura, N., Mochizuki, K., Kamachi, T., Okura, I
    • Journal Title

      Measurement Science and Technology 17

      Pages: 1237-1241

  • [Journal Article] 「研究成果報告書概要(欧文)」より2006

    • Author(s)
      Ihara, M., Nakamoto, H., Kamachi, T., Okura, I
    • Journal Title

      Photochemistry and Photobiology 82

      Pages: 1677-1685

  • [Journal Article] 「研究成果報告書概要(欧文)」より2006

    • Author(s)
      Ihara, M., Nishihara, H., Yoon, K S., Lenz, O., Friedrich, B., Nakamoto, H., Colima, K, Honma, D., Kamachi, T., Okura, I
    • Journal Title

      Photochemistry and Photobiology 82

      Pages: 676-682

  • [Journal Article] 「研究成果報告書概要(欧文)」より2006

    • Author(s)
      Iida, S., Asakura, N., Tabata, K., Okura, I., Kamachi, T
    • Journal Title

      Chera BioChem 7

      Pages: 1853-1855

  • [Journal Article] 「研究成果報告書概要(欧文)」より2006

    • Author(s)
      Ogura, S., I., Tabata, K., Fukushima, K., Kamachi, T., Okura, I
    • Journal Title

      Journal of Porphyrins and Phthalocyanines 10

      Pages: 1116-1124

  • [Journal Article] 「研究成果報告書概要(欧文)」より2006

    • Author(s)
      Tamura, A., Watanabe, M., Saito, H., Nakagawa, H., Kamachi, T., Okura, I., Ishikawa, T
    • Journal Title

      Molecular Pharmacology 70

      Pages: 287-296

  • [Presentation] Photoinduced electron transfer via photoexcited singlet state of hydrophobic porphyrin in aqueous solution2008

    • Author(s)
      Yoichi Okawa, Atuhiro Tokita, Noriyuki Asakura, Toshiaki Kamachi, Ichiro Okura
    • Organizer
      Global COE in chemistry, Nagoya Special symposium
    • Place of Presentation
      名古屋
    • Year and Date
      2008-03-27
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Preparation of one heme removed cytochrome c3, and reconstitution with FePPIX2008

    • Author(s)
      Takuya Okada, Noriyuki Asakura, Kenji Tabata, Toshia ki Kamachi, Ichiro Okura
    • Organizer
      Global COE in chemistry, Nagoya special symposium
    • Place of Presentation
      名古屋
    • Year and Date
      2008-03-27
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Photoinduced electron transfer via photoexcited singlet state of hydrophobic porphyrin in aqueous solution2008

    • Author(s)
      Yoichi, Okawa, Atuhiro, Tokita, Noriyuki, Asakura, Tbshiaki, Kamachi, Ichiro, Okura
    • Organizer
      Global COE in chemistry, Nagoya special symposium
    • Year and Date
      2008-03-27
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Preparation of one heme removed cytochrome c3, and reconstitution with FePPIX2008

    • Author(s)
      Takuya, Okada, Noriyuki, Asakura, Kenji, Tabata, Toshiaki, Kamachi, Ichiro, Okura
    • Organizer
      Global COE in chemistry, Nagoya special symposium
    • Year and Date
      2008-03-27
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Spectroscopic propaties of methane monooxygenase from Meth ylosinus trichosporium2007

    • Author(s)
      Tomonori Yaguchi, Masaru Saitou, Noriyuki Asakura, Kenji Tabata, Toshiaki Kamachi, Ichiro Okura
    • Organizer
      SPACC-14
    • Place of Presentation
      Taipei
    • Year and Date
      2007-12-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Inhibitory mechanism of propargylamine on particulate methane monooxygenase from Methylosinus trichosporium OB3b2007

    • Author(s)
      Kenji Tabata, Tomonori Yaguchi, Takeyori Harashina, Noriyuki Asakura, Toshiaki Kamachi, Ichiro Okura
    • Organizer
      SPACC-14
    • Place of Presentation
      Taipei
    • Year and Date
      2007-12-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Ichiro Okura Spectroscopic propaties of methane mononnmenase frnm Mcthvincinnc trirhnennAlirn 11R2h2007

    • Author(s)
      Tomonori, Yaguchi, Masaru, Saitou, Noriyuki, Asakura, Kenji, Tabata, Toshiaki, Kamachi
    • Organizer
      SPACC-14
    • Place of Presentation
      Taipei
    • Year and Date
      2007-12-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] メタン高度化学変換技術集成(分担)、「膜結合型メタンモノオキシゲナーゼを利用したメタンからの環境低負荷型メタノール生産技術の開発」2008

    • Author(s)
      田畠健治, 蒲池利章.
    • Total Pages
      13
    • Publisher
      シーエムシー
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] トコトンやさしいタンパク質の本2007

    • Author(s)
      蒲池利章
    • Total Pages
      3
    • Publisher
      日刊工業新聞社
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] ナノバイオ計測の実際-EQCM測定-2007

    • Author(s)
      朝倉則行, 蒲池利章
    • Total Pages
      14
    • Publisher
      講談社
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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