2007 Fiscal Year Final Research Report Summary
Development of Neutrophile Regulatory Peptides with Receptor Association Structures
| Project/Area Number |
18550154
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Saga University |
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Principal Investigator |
KODAMA Hiroaki Saga University, Chemistry, Associate Professor (80205418)
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| Co-Investigator(Kenkyū-buntansha) |
OSADA Satoshi Saga University, Chemistry, Assistant Professor (50284609)
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| Project Period (FY) |
2006 – 2007
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| Keywords | signal transduction / biomolecular / biological activity / protein / dimer |
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| Research Abstract |
Neutrophil functions including chemotaxis, degranulation, and generation of superoxide anion are modulated by diverse extracellular agonists such as N-formyl-methionyl-leucyl-phenylalanine (fMLP). Formyl peptide receptor (FPR) and formyl peptide receptor-like 1 (FPRL1), a superfamily of seven transmembrane (TM) proteins, are expressed on human neutrophils as the fMLP binding receptors. We found that human neutrophils pretreated with human formyl peptide receptor transmembrane (hFPRTM) peptides were enhanced superoxide anion production when stimulated with fMLP. However, a membrane protein interacting with hFPRTM peptides is not identified. To explore the sequence dependences of the TM peptides for neutrophil proming activities, peptides possess the TM sequences of formyl peptide receptor (FPR), FPR like 1 receptor (FPRL1), and GABA receptor were synthesized by solid-phase method with Fmoc chemistry. Homogeneities and structures of synthetic peptides were confirmed by HPLC and MALDI-TOF MS. The biological activities of synthetic peptides were carried out as superoxide production for human neutrophils. Neutrophils treated with TM peptides from FPR and FPRL1 produced 2-3 folds of superoxide anion by the treatment of fMLP, neutrophil agonist. TM peptide from GABA receptor exhibited no significant priming activity. These results suggested the priming effect of TM peptide for neutrophil was sequence dependence and it required the FPR related sequences.
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[Journal Article] Combination therapy of an anticancer drug with the FNIII14 peptide of fibronectin effectively overcomes cell adhesion-mediated drug resistance of acute myelogenous leukemia2008
Author(s)
T. Matsunaga, F. Fukai, S. Miura, Y. Nakane, T. Owaki, H. Kodama, M. Tanaka, T. Nagaya, R. Takimoto, T. Takayama, Y. Niitsu
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Journal Title
Leukemia 22
Pages: 353-360
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Combination therapy of an anticancer drug with the FNIII14 peptide of fibronectin effectively overcomes cell adhesion-mediated drug resistance of acute myelogenous leukemia2008
Author(s)
T., Matsunaga, F., Fukai, S., Miura, Y., Nakane, T., Owaki, H., Kodama, M., Tanaka, T., Nagaya, R., Takimoto, T., Takayama, Y., Niitsu
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Journal Title
Leukemia 22
Pages: 353-360
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Antiadhesive sites present in the fibronectin type III-like repeats of human plasma fibronectin2007
Author(s)
S., Miura, S., Kamiya, Y., Saito, S., Wada, R., Hayashi, J., Taira, H., Kodama, H., Yajima, M., Ueki, F., Fukai
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Journal Title
Biol. Pharm. Bull 30
Pages: 891-897
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Synthesis of Transmembrane Peptides of GPCR Type Receptors and Nneutrophil Activations2008
Author(s)
D., Sugiyama, D., Shibata, S., Osada, Y., Hamasaki, I., Fujita, H., Kodama
Organizer
Annual Meeting of Japan Chemical Society
Place of Presentation
Tokyo
Year and Date
20080326-30
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Synthesis and biological activities of a peptide derived from formyl peptide receptors2007
Author(s)
D., Sugiyama, D., Shibata, S., Osada, Y., Hamasaki, I., Fujita, H., Kodama
Organizer
BMB2007
Place of Presentation
Yokohama
Year and Date
20071211-15
Description
「研究成果報告書概要(欧文)」より
