2007 Fiscal Year Final Research Report Summary
Development of novel spin-labeled nucleic acids for detection of DNA internal dynamics
| Project/Area Number |
18551052
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| Allocation Type | Single-year Grants |
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| Research Institution | Kyushu University |
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Principal Investigator |
ASO Mariko Kyushu University, Graduate School of Pharmceutical Sciences, Assistant Professor (30201891)
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| Co-Investigator(Kenkyū-buntansha) |
SUEMUNE Hiroshi KYUSHU UNIVERSITY, Graduate School of Pharmceutical Sciences, Professor (20095897)
KATAYAMA Tsutomu KYUSHU UNIVERSITY, Graduate School of Pharmceutical Sciences, Professor (70264059)
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| Project Period (FY) |
2006 – 2007
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| Keywords | spin-label / ESR / DNA / dynamics |
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| Research Abstract |
We synthesized oligodeoxynucleotides (ODN, 15-mer and 18-mer) containing 2-N-tert-butylaminoxyladenosine (1) and measured their ESR spectra in single-and double-strand forms. We calculated 2Azz values and h_+/h_0, values (h_+; height of the low field peak, h_0; height of the center peak) to evaluate mobility of ODNs. Upon formation of duplex, increase of 2Azz value and decrease of h_+/h_0 value were observed to indicate the mobility of ODN decreased. The 2Azz and h/h_0 values of single-stranded ODN at 25℃ were identical with those at 10 ℃. In contrast, the 2Azz and h_+/h_0 values of duplex at 25 and 10 ℃ changed. That indicated the mobility of duplex is more sensitive to temperature than single-stranded ODN.
ESR spectra of duplex between ODN containing 1 and complementary strands which contain A, C, G T at the opposite position to 1 indicated possibilities to report duplex stabilities.
ESR spectra of duplex between ODN containing 1 and complementary strands which contain mismatches indicated possibilities to report duplex stabilities.
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