2007 Fiscal Year Final Research Report Summary
Detection and analysis of new functional genes on the genome of bacterio phages
| Project/Area Number |
18570001
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Genetics/Genome dynamics
|
|---|
| Research Institution | Saitama University |
|---|
Principal Investigator |
SADAIE Yoshito Saitama University, Graduate school of Science and Engineering, Professor (40000252)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ASAI Kei Saitama University, Graduate school of Science and Engineering, Associate Professor (70283934)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Keywords | Bacillus subtilis / bacteriophage / restriction modification system / genomics |
|---|
| Research Abstract |
When the bacteriophage is infected with specific host bacteria, it become lysogeny in a host chromosome or multiplies in a host cell and finally causes lysis of a host. The prophage genes which become lysogeny been mutated afterwards, and these can bring a host a new function. Many of protein encoded by a gene on the bacteriophage DNA are novel, and we can discover useful genes of unknown function and structure. We begin this research aiming at search and analysis of such novel genes.
Bacillus subtilis Maeberg is tolerance to bacteriophage SP10, but it becomes sensitive when the nonA and the nonB mutations are occurred in B. subtilis cells. We started this study to elucidate this phenomenon.
I performed the sequencing of the SP10 bacteriophage genome to analyze mechanism for the infection of SP10 bacteriophage. For making librarye, SP10 genomic DNA was sheared by plural restriction enzymes and supersonic waves. These fragments were cloned into a plasmid vector and sequenced. These sequenced data were assembled and made some contig. 72 contig was provided by the present and 97, 547bp of 90% in total length (about 108kb) were determined by sequencing. High similarities with Staphylococcus phage K ORF were often observed. We tried to determine whole genome sequence now.
The nonA locus is lied in SP□, B. subtilis prophage. We deleted gradually SP□ out from B. subtilis chromosome and tested their sensitivity to SP10 to determine the gene which causes nonA phenotype. As a result of this analysis, it became clear that there was plural mechanism. There are at least two mechanism concerned. One is yonO gene and non-identified genes, and another is homolog of the dNDP reductase. The function of yonO gene is unknow, but is possibly regulator of gene expression. Future analysis is still to be elucidating.
|
