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2007 Fiscal Year Final Research Report Summary

Roles of condensin complexes in the maintenance and functional expression of genome

Research Project

Project/Area Number 18570009
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Genetics/Genome dynamics
Research InstitutionThe Institute of Physical and Chemical Research (2007)
Institute for Developmental Research, Aichi Human Service Center (2006)

Principal Investigator

ONO Takao  The Institute of Physical and Chemical Research, Chromosome Dynamics Laboratoty, Senior Research Scientist (20291172)

Project Period (FY) 2006 – 2007
KeywordsChromosome / Condensin / Chromosomal aberration / Cell Cycle / G-band / Histone modification / DNA replication
Research Abstract

In higher eukaryotic cells, there exist two condensin complexes, known as condensin I and II. Both play essential yet distinct functions in the processes of mitotic chromosome assembly and segregation. In this study, we have investigated chromosomal aberrations caused by the defect of chromosome assembly and segregation. Further, potential factors that might contribute to the distinct dynamics of condensin I and II have been searched.
(1) We found that clinical cytogenetic chromosome preparations is not useful for defection of chromosomal defects associated with chromosome assembly and segregation. On the other hand, combination of condensin-depletion and replicative stress caused severe morphological defects in mitotic chromosomes of Hela cells. These results suggest that condensin may play a role in protection or maintenance of chromosomal structure in addition to chromatin assembly. Moreover, unknown factor (s) might contribute to the formation of chromosome morphology.
(2) We have established a new a cytological method, terms monopolar preparation, for clinical analysis of human diseases associated with defects of chromosome assembly and segregation.
(3) We have found that condensins I and II tend to be enriched in R-band and G-band regions, respectively, on metaphase chromosomes. Further, condensin distribution pattern also seems to be related with histone modifications.
(4) We demonstrated that condensin II may bind to chromatin during S-phase in HeLa cells. This obsevation allows us to propose the working hypothesis that condensin II may play a role in coordinating two distinct chromosome events, i., e., DNA replication in S phase and chromosome assembly in M phase.

  • Research Products

    (18 results)

All 2008 2007 2006 Other

All Journal Article (6 results) (of which Peer Reviewed: 2 results) Presentation (12 results)

  • [Journal Article] Key roles of condensins in mitotic chromosome assembly and segregation.2007

    • Author(s)
      Takao Ono
    • Journal Title

      Chrom. Sci. 9

      Pages: 33-45

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Monopolar preparation of human lymphocytes for evaluation of the metaphase chromosome alignment.2007

    • Author(s)
      Reiko Kimura
    • Journal Title

      Chrom. Sci. 9

      Pages: 75-83

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] 見えない染色体異常-染色体構築と分配機構の異常による先天性疾患-2007

    • Author(s)
      小野教夫
    • Journal Title

      実験医学 25

      Pages: 776-781

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Invisible chromosome disorder : Congenital diseases associated with defects of chromosome assembly and segregation.(in Japanese)2007

    • Author(s)
      Ono T, Kimura R, Yamada K, Wakamatsu N.
    • Journal Title

      Experimental Medicinee 25

      Pages: 776-781

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Key roles of condensins in mitotic chromosome assembly and segregation.2006

    • Author(s)
      Ono T.
    • Journal Title

      Chrom. Sci. 9

      Pages: 33-45

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Monopolar preparation of human lymphocytes for evaluation of the metaphase chromosome alignment.2006

    • Author(s)
      Kimura R, Takeshima K, Mizuno S, Kosaki K, Machida J, Kamamoto M, Muro Y, Shimosato K, Wakamatsu N, Sonta S, Ono T.
    • Journal Title

      Chrom. Sci. 9

      Pages: 75-83

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 2つのコンデンシンの時空間制御の理解へ向けて.2008

    • Author(s)
      小野教夫
    • Organizer
      第25回染色体ワークショップ
    • Place of Presentation
      静岡県熱海市(ウェルシティ湯河原)
    • Year and Date
      2008-02-01
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Disorders in Chromosome Assembly : New Chromosomal Aberrations Associated with Human Congenital Diseases.2007

    • Author(s)
      Takao Ono
    • Organizer
      The 8th international symposium on Chromosomal Aberrations.
    • Place of Presentation
      兵庫県淡路市(淡路夢舞台国際会議場)
    • Year and Date
      2007-10-05
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Disorders in Chromosome Assembly : New Chromosomal Aberrations Associated with Human Congenital Diseases.2007

    • Author(s)
      Ono T.
    • Organizer
      The 8th international symposium on Chro mosomal Aberrations.
    • Place of Presentation
      Awaji, Hyogo Pref. Japan
    • Year and Date
      2007-10-05
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 染色体構築のしくみと疾患2007

    • Author(s)
      小野教夫
    • Organizer
      日本人類遺伝学会第14回臨床細胞遺伝学セミナー
    • Place of Presentation
      家の光会館(東京都新宿区)
    • Year and Date
      2007-08-26
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Chromosome assembly and human diseases.2007

    • Author(s)
      Ono T.
    • Organizer
      The Japan Society of Human Genetics. The 14th Seminar for Clinical Cytogenetics.
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2007-08-26
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 単極性染色体整列法を用いた染色体構築と分離異常の検索2006

    • Author(s)
      小野教夫
    • Organizer
      染色体学会第57回年会
    • Place of Presentation
      千葉県千葉市稲毛区(千葉大学)
    • Year and Date
      2006-11-24
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Analysis of chromosomal aberration in their assembly and segregation2006

    • Author(s)
      Ono T, Takeshima K, Mizuno S, Kamamoto M, Kimura R.
    • Organizer
      The Society of Chromosome Research. The 57th Annual Meeting.
    • Place of Presentation
      Chiba, Japan
    • Year and Date
      2006-11-24
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Chromosome abnormality caused by depletion of condensins2006

    • Author(s)
      Takao Ono
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      京都府京都市(京都国際会議場)
    • Year and Date
      2006-06-19
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Chromosome abnormality caused by depletion of condensins.2006

    • Author(s)
      Ono T, Kimura R.
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress.
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2006-06-19
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 染色体構築機構とその異常2006

    • Author(s)
      小野教夫
    • Organizer
      日本環境変異原学会MMS研究会第49回定例会
    • Place of Presentation
      静岡県熱川市
    • Year and Date
      2006-05-19
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Molecular mechanism of chromosome assembly and chromosomal aberration.2006

    • Author(s)
      Ono T.
    • Organizer
      The Japanese Environmental Mutagen Society, Mammalian Mutagenicity Study Group Society. The 49th Annual meeting
    • Place of Presentation
      Atagawa, Shizuoka Pref. Japan
    • Year and Date
      2006-05-19
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Toward an understanding of the spatial and temporal regulation of two condensins.

    • Author(s)
      Ono T, Hirano T.
    • Organizer
      The 25th Chromosome Workshop.
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-02-04  

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