2007 Fiscal Year Final Research Report Summary
Roles of condensin complexes in the maintenance and functional expression of genome
| Project/Area Number |
18570009
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | The Institute of Physical and Chemical Research (2007)
Institute for Developmental Research, Aichi Human Service Center (2006) |
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Principal Investigator |
ONO Takao The Institute of Physical and Chemical Research, Chromosome Dynamics Laboratoty, Senior Research Scientist (20291172)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Chromosome / Condensin / Chromosomal aberration / Cell Cycle / G-band / Histone modification / DNA replication |
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| Research Abstract |
In higher eukaryotic cells, there exist two condensin complexes, known as condensin I and II. Both play essential yet distinct functions in the processes of mitotic chromosome assembly and segregation. In this study, we have investigated chromosomal aberrations caused by the defect of chromosome assembly and segregation. Further, potential factors that might contribute to the distinct dynamics of condensin I and II have been searched.
(1) We found that clinical cytogenetic chromosome preparations is not useful for defection of chromosomal defects associated with chromosome assembly and segregation. On the other hand, combination of condensin-depletion and replicative stress caused severe morphological defects in mitotic chromosomes of Hela cells. These results suggest that condensin may play a role in protection or maintenance of chromosomal structure in addition to chromatin assembly. Moreover, unknown factor (s) might contribute to the formation of chromosome morphology.
(2) We have established a new a cytological method, terms monopolar preparation, for clinical analysis of human diseases associated with defects of chromosome assembly and segregation.
(3) We have found that condensins I and II tend to be enriched in R-band and G-band regions, respectively, on metaphase chromosomes. Further, condensin distribution pattern also seems to be related with histone modifications.
(4) We demonstrated that condensin II may bind to chromatin during S-phase in HeLa cells. This obsevation allows us to propose the working hypothesis that condensin II may play a role in coordinating two distinct chromosome events, i., e., DNA replication in S phase and chromosome assembly in M phase.
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Research Products
(18 results)
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[Journal Article] Monopolar preparation of human lymphocytes for evaluation of the metaphase chromosome alignment.2006
Author(s)
Kimura R, Takeshima K, Mizuno S, Kosaki K, Machida J, Kamamoto M, Muro Y, Shimosato K, Wakamatsu N, Sonta S, Ono T.
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Journal Title
Chrom. Sci. 9
Pages: 75-83
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 染色体構築のしくみと疾患2007
Author(s)
小野教夫
Organizer
日本人類遺伝学会第14回臨床細胞遺伝学セミナー
Place of Presentation
家の光会館(東京都新宿区)
Year and Date
2007-08-26
Description
「研究成果報告書概要(和文)」より
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[Presentation] 染色体構築機構とその異常2006
Author(s)
小野教夫
Organizer
日本環境変異原学会MMS研究会第49回定例会
Place of Presentation
静岡県熱川市
Year and Date
2006-05-19
Description
「研究成果報告書概要(和文)」より
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