2007 Fiscal Year Final Research Report Summary
Molecular genetic analysis and screen for nascent-peptide mediated regulation in Arabidopsis
| Project/Area Number |
18570032
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | Hokkaido University |
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Principal Investigator |
NOUCHI Hitoshi Hokkaido University, Res. Fac. of Asso, Professor (50322839)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Arabidopsis / upstream ORF / translation / posttranscriptional regulation / ribosome |
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| Research Abstract |
In the course of study on the posttranscriptional regulation of the Arabidopsis CGS1 gene, which encodes the key enzyme of methionine biosynthesis, we have found that the nascent CGS1 peptide mediates its own translation elongation arrest in response to S-adenosylmethionine, a metabolite of methionine. Although several examples of nascent peptide-mediated ribosomal regulations have been reported in eukaryotes, all other regulatory nascent peptides are encoded by upstream ORFs (uORF) located in the 5'UTR, except for the CGS1 gene. In these systems, uORF-encoded peptides cause ribosome stalling, resulting in downregulation of translation of the main ORF.
In order to identify new Arabidopsis genes that are regulated by nascent peptide, we screened for uORF-containing genes from Arabidopsis full-length cDNA database, and tested their amino acid-sequence dependency of the effect on translation of the main ORF by using transient assay with Arabidopsis protoplasts. From this screen, a frameshift mutation of one uORF was found to cause upregulation of the main ORF. Alanine scanning analysis showed that C-terminal part of the peptide encoded by this uORF is important for the regulation. In contrast, a frameshift mutation of another uORF caused downregulation of the main ORF.
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Research Products
(14 results)
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[Journal Article] Nascent peptide-mediated translation elongation arrest of Arabidopsis thaliana CGS1 mRNA occurs autonomously.2008
Author(s)
Onouchi, H., Haraguchi, Y., Nakamoto, M., Kawasaki, D., Nagami-Yamashita, Y., Murota, K., Kezuka-Hosomi, A., Chiba, Y. and Naito, S.
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Journal Title
Plant Cell Physiol 49
Pages: 549-556
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Translation elongation arrest and coupled mRNA decay mechanisms for regulation of the Arabidopsis CGS1 gene that encodes a key enzyme in methionine biosynthesis.2007
Author(s)
Onouchi, H., Haraguchi, Y., Nakajima, K., Murota, .K., Kadokura, Y., Kawasaki, D., Hirata, K., Nagami, Y., and Naito, S.
Organizer
The 48th Annual Meeting of the Japanese Society of Plant Physiologists.
Place of Presentation
Matsuyama University.
Year and Date
2007-03-29
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Post-transcriptional regulation of cystathionine γ-synthase: Analyzing the role of the ribosomal exit tunnel.2007
Author(s)
Hasegawa, S., Nakajima, K., Murota, .K., Onoue, N., Onouchi, H., and Naito, S.
Organizer
The 48th Annual Meeting of the Japanese Society of Plant Physiologists.
Place of Presentation
Sapporo Convention Center.
Year and Date
2007-03-21
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Regulation of the Arabidopsis CGS1 mRNA stability coupled with translation elongation arrest.2006
Author(s)
Onouchi, H., Haraguchi, Y., Nakajima, K., Murota, .K., Kadokura, Y., Kawasaki, D., Hirata, K., Nagami, Y., and Naito, S.
Organizer
Molecular Biology Society of Japan 2006 Forum.
Place of Presentation
Nagoya Congress Center
Year and Date
2006-12-08
Description
「研究成果報告書概要(欧文)」より
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