2007 Fiscal Year Final Research Report Summary
Analysis of chloroplast division mechanism by genome information, gene expression profile and reverse genetics.
| Project/Area Number |
18570036
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
SHIMADA Hiroshi Tokyo Institute of Technology, Graduate School of Bioscience and Biotechnology, Assistant Professor (80301175)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Genome / Plant / Regulation of expression / Chloroplast |
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| Research Abstract |
I selected 120 genes that are co-expressed with ARC3 and FtsZ genes, chloroplast division factor, using a gene expression profile in Arabidopsis thaliana, and I got the T-DNA line of the each gene from a stock center. I screened the homo lines of T-DNA insertion from the seeds by PCR. A number or size of chloroplasts in the homo line cells were observed with an optical microscope. The T-DNA lines of At1g56050 and At1g26230 had a small number of abnormally large chloroplasts in the cells, suggesting that At1g56050 and At1g26230 were the novel chloroplast division factors. The interaction of ARC3 protein and At1g56050 or At1g26230 were analyzed with yeast two-hybrid system. The results indicated that At1g56050 protein interacted with ARC3 protein. It was suggested that At1g56050 (GTP-binding protein) was the novel chloroplast division factor.
DNA array analysis revealed that gene expression profile between wild type and arc3 mutant plants was almost same but the expression of At5g42825 in the mutant was higher than that of wild type. At5g42825 gene has no clear protein coding region, suggesting that At5g42825 might have a function as native RNAi.
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[Journal Article] Arabidopsis cotyledon-specific chloroplast biogenesis factor CYO1 is a protein disulfide isomerase2007
Author(s)
H., Shimada, H., Mochizuki, M., Ogura, K., Froehlich, J. E., Osteryoung, K. W., Shirano, Y., Shibata, D., Masuda, S., Mori, K. and Takamiya, K.
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Journal Title
The Plant Cell 19
Pages: 3157-3169
Description
「研究成果報告書概要(欧文)」より
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