2007 Fiscal Year Final Research Report Summary
Fundional of non-coding RNAs in adaptation of cyanobacteria to stress conditions
| Project/Area Number |
18570039
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | Nagoya University |
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Principal Investigator |
OMATA Tatsuo Nagoya University, Graduate School of Bbag ricutural Sciences, Professor (50175270)
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| Co-Investigator(Kenkyū-buntansha) |
FUJITA Yuichi Nagoya University, Graduate School of Biioagricubaal Sdenoes, Associate Professor (80222264)
MAEDA Shin-icri Nagoya University, Graduate School of Bioagricutural Sdences, Associate Professor (70335016)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Gene expression / Cyanobacteria / Genome / Environmental stress / Microarray |
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| Research Abstract |
1. Identification of ncRNA genes responsive to environmental stress.
We searched for low-CO_2-inducible ncRNA genes in the cyanobacterium Synechococcus elongatus PCC 7942, using DNA microarrays that have oligonucleotide spots for both strands of ORFs and intergenic regions. Ten intergenic regions, whose transcription levels were high and whose expression patterns were different from'those of the adjacent ORFs, were selected as candidates of the regions harboring the ncRNA genes. In two of these regions, low-CO_2-inducible small transcripts were detected by Northern blot analysis, confirming the occurrence of ncRNAs induced by CO_2 limitation.
2. Mechanisms of induction of the ncRNA genes.
The transcription start points of the two ncRNA genes were determined. The deduced promoter region of one of the ncRNA genes had an HLR motif typical of high-light responsive genes as well as a CbbR motif (TnA-n7-TnA) found in many low CO_2-resonsive genes. This ncRNA gene was shown to respond to high light as well as low CO_2. The other ncRNA gene had no typical promoter elements nor known cis-acting elements for low-CO_2-induced genes.
3. Role of the ncRNAs in adaptation of cyanobacterial cells to CO2 limitation.
Using an expression vector, the complementary strands of the ncRNA were overexpressed in Synechococcus elongatus cells. The transformants grew slower than the wild-type strain or the strain harboring the empty vector under low CO_2-conditins. The results suggested that the ncRNAs help growth of the cyanobacterium under CO_2- limited growth conditions
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[Journal Article] Mechanism of low-CO_2 induced activation of the cmp bicarbonate transporter operon by a LysR family protein in the cyanobacterium Synechococcus elongatis strain PCC 7942.2008
Author(s)
Nishimura, T., Takahashi, Y, Yamaguchi, O., Suzuki, H., Maeda, S. and Omata, T.
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Journal Title
Molecular Microbiology 68
Pages: 98-109
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Mechanism of low-CO2 induced activation of the cmp bicarbonate transporter operon by a LysR family protein in the cyanobacterium Synechococcus elongatus strain PCC 79422008
Author(s)
Nishimura, T., Takahashi, Y, Yamaguchi, O., Suzuki, H., Maeda., S., Omata., T
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Journal Title
Molecular Microbiology 68
Pages: 98-109
Description
「研究成果報告書概要(欧文)」より
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