2007 Fiscal Year Final Research Report Summary
Reevaluation of the role of chloroplasts in the calsium signaling in guard cells
| Project/Area Number |
18570048
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | Kyoto Prefectural University |
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Principal Investigator |
SHIINA Takashi Kyoto Prefectural University, Faculty of Human environment, Professor (10206039)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Ca^<2+> / stomata / CAS / chlorosplast / infection / stress / chloroplast signal / Arabidopsis |
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| Research Abstract |
The role of guard cell chloroplasts in stomatal function is controversial. It is usually assumed that stomatal closure is preceded by a transient increase in cytosolic free Ca^<2+> concentration (Ca^<2+>)_<cyt>) in the guard cells. CAS (Ca^<2+> sensing receptor) is a plant-specific putative Ca^<2+>-binding protein. We foundthat CAS is an integral thylakoid membrane protein, and the N-terminus acidic Ca^<2+>-binding region is likely exposed to the stromal side of the membrane. CAS is essential for stomatal closure induced by external Ca^<2+>. These results provide the evidence that chloroplasts play a critical role in the generation of extracellular Ca^<2+>([Ca^<2+>]_<ext>)-induced[Ca^<2+>]_<cyt> transients and stomatal closure in Arabidopsis.
In addition, we founs that extracellular abiotic and pathogen-related stress signals are relayed quickly to chloroplasts, and evoke rapid and transient increases in stromal Ca^<2+> concentration in a stress-dependent manner. Furthermore we demonstrated that CAS is also related to the generation of stromal Ca2+ transients induced by pathogen elicitors and/or light to dark transition. Furthermore, we demonstrated that CAS regulates a multitude of plant innate immune responses, such as flg22-induced stomatal closure, expression of pathogen-related genes, ROS production and a resistance (R) gene-triggered hypersensitive cell death in Arabidopsis. It should be noted that cas-1 shows no significant growth phenotype. These results may have implications for a far more important role of chloroplasts in the regulation of plant innate immune response than was thought.
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[Journal Article] AtObgC, a plant ortholog of bacterial Obg, is a chloroplast-targeting GTPase essential for early embryogenesis.2009
Author(s)
Bang WY, Hata A, Jeong IS, Umeda T, Masuda T, Chen J, Yoko I, Suwastika IN, Kim DW, Im CH, Lee BH, Lee Y, Lee KW, Shiina T, Bahk JD.
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Journal Title
Plant Mol Biol. 71
Pages: 379-390
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] AtObgC, a plant ortholog of bacterial Obg, is a chloroplast-targeting GTPase essential for early embryogenesis2009
Author(s)
Bang WY, Hata A, Jeong IS, Umeda T, Masuda T, Chen J, Yoko I, Suwastika IN, Kim DW, Im CH, Lee BH, Lee Y, Lee KW, Shiina T, Bahk JD.
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Journal Title
Plant Mol Biol. 71
Pages: 379-390
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Role of Arabidopsis CHL27 protein for photosynthesis, chloroplast development and gene expression profiling.2008
Author(s)
Bang WY, Jeong IS, Kim DW, Im CH, Ji C, Hwang SM, Kim SW, Son YS, Jeong J, Shiina T, Bahk JD.
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Journal Title
Plant Cell Physiol. 49
Pages: 350-363
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Role of Arabidopsis CHL27 protein for photosynthesis, chloroplast development and gene expression profiling2008
Author(s)
Bang WY, Jeong IS, Kim DW, Im CH, Ji C, Hwang SM, Kim SW, Son YS, Jeong J, Shiina T, Bahk JD.
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Journal Title
Plant Cell Physiol. 49
Pages: 350-63
Description
「研究成果報告書概要(欧文)」より
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