2007 Fiscal Year Final Research Report Summary
Studies on Diversity and Universality in Sperm Activation and Chemotaxis at Fertilization
| Project/Area Number |
18570080
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | Yamagata University |
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Principal Investigator |
MORISAWA Masaaki Yamagata University, Faculty of Science, Professor (40013594)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Akihiko Yamagata University, Faculty of Science, Associate Professor (30250913)
HISINUMA Tasuku Yamagata University, Faculty of Science, Lecturer (70181097)
YOSHIDA Manabu The University of Tokyo, Graduate School of Science, Lecturer (60301785)
MOTOMURA Taizou Hokkaido University, Graduate School of Science, Professor (30183974)
IWAMOTO Teruaki International University of Health and Welfare, 国際医療福祉大学, Professor (60046117)
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| Project Period (FY) |
2006 – 2007
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| Keywords | sperm activation / sperm chemotaxis / human / amphibians / sea cucumber / starfish / ascidians / brown algae |
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| Research Abstract |
Sperm activating substance derived from human follicular fluid was partially purified, but chemical structure of it is still obscure. The role of semenogelin and zinc ions for the initiation of human sperm motility was investigated and the obtained results were publishing in the international scientific journal "Human Reproduction". Purification and identification of molecular structure of sperm activating peptides were tried in newt. Furthermore, the interesting results that newt derived sperm activating peptide also activates sperm of salamander and primitive toad were obtained by collaboration work with Italian scientists. Immuno-histochemical analysis showed that the sperm activating peptides from newt, salamander and toad are similar immunological properties. Collaboration works with American scientists showed that calucium ions has an important role in the cell signaling mechanism for sperm activation in newt. In regards with species specificity in sperm activation and chemotaxis in ascidians, there was clear species specificity between genus. Furthermore, Purification processes of sperm activating and attracting factors were successfully proceeded in the two ascidian species, Phallusia mamillata and Ascidia sydneiensis. Sperm activating substance from jelly layer of sea cucumber eggs was partially purified, and it was identified that the substance will be small peptide. Physiological analyses on the-mechanism of the initiation of sperm motility in starfish have clearly shown that a zinc binding enzyme, carbonic anhydrase regulates intracellular pH by controlling C0_2/HCO_3 for triggering the phenomenon. We finally tried to investigate the outline of cell signaling for sperm activation and chemotaxis in brown algae using software for sperm motility analysis, Boh Boh.
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Research Products
(14 results)
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[Journal Article] Physiological roles of semenogelin I and zinc in sperm motility and semen coagulation on ejaculation in humans2008
Author(s)
Yoshida, K, ・Kawano, N, ・Yoshiike, M, ・Yoshida, M, ・Iwamoto, T, ・Morisawa, M
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Journal Title
Mol. Human Reprod. 14
Pages: 151-156
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Role of two egg-dericed molecules in motility initiation and fertilization in the Pacific herring(Clupea pallasi).2008
Author(s)
Cherr, GN., ・Morisawa, M., ・Vines, CA., ・Yoshida, K., ・Smith, EH, ・Matsubara, T, ・Pillai, MC., ・Griffin, FJ.・Yanagimachi, R.
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Journal Title
Intern. J. Develop. Biol, (印刷中)
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Motility activation and chemotaxis in ascidian sperm.2008
Author(s)
Morisawa, M., ・Yoshida, M., ・lnaba, K., ・lzumi, H., ・Nomura, M. ・Murata, M.
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Journal Title
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] activation and chemotaxis in ascidian sperm2008
Author(s)
Morisawa, M., Yoshida, M., Inaba, K., Izumi, H., Nomura, M. and Murata, M. Motility
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Journal Title
Front. BioSci. (in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Role of the Na-/Ca^<2+> exchanger in calcium homeostasis and human sperm motility regulation.2006
Author(s)
Krasanai, Z., ・Krasznai, ZT., ・Morisawa, M., ・Bazsanekassai, Zs., ・Hernadi, Z., ・Fazekas, ZS., ・Tron, L., ・Goda, K., ・Marian, T.
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Journal Title
Cell Motil. Cytoskelt. 63
Pages: 66-76
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Na+/Ca^<2+> exchanger modulates the flagellar wave pattern for the regulation of motility activation and chemotaxis in the ascidian spermatozoa.2006
Author(s)
Shiba, K., ・Marian, T., ・Krasznai, Z., ・Baba, SA., <Morisawa, M.・Yoshida, M.
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Journal Title
Cell Motile, Cytoskelt., 63
Pages: 623-632
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Role of the Na^<+>/Ca^<2+> exchanger in calcium homeostasis and human sperm motility regulation2006
Author(s)
Krasznai, Z., Krasznai, ZT, Morisawa, M., Bazsane Kassai, ZS., Hernadi, Z., Fazekas, ZS., Tron, L., Goda, K., Marian, T.
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Journal Title
Cell Motil. Cytoskel. 63
Pages: 66-76
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Na^<+>/Ca2+ exchanger modulates the flagellarwave pattern for the regulation of motility activation and chemotaxis in the ascidian spermatozoa2006
Author(s)
Shiba, K., Marian, T., Krasznai, Z., Baba, SA., Morisawa, M. and Yoshida, M.
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Journal Title
Cell Motile, Cytoskel 63
Pages: 623-632
Description
「研究成果報告書概要(欧文)」より
