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2007 Fiscal Year Final Research Report Summary

Nuclear function of protein kinase CK2 on cell cycle regulation

Research Project

Project/Area Number 18570133
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionFukushima Medical University

Principal Investigator

KATO Miwako  Fukushima Medical University, SCHOOL OF MEDICINE, DEPARTMENT OF BIOMOLECULAR SCIENCE, LECTURER (40192538)

Project Period (FY) 2006 – 2007
KeywordsCK2 / Cell cycle / nucleus / hnRNP / 細胞核
Research Abstract

Casein kinase 2 (CK2) is a highly conserved and ubiquitous eukaryotic Ser/Thr protein kinase. Genetic, biochemical and cell biological studies have indicated the involvement of this enzyme in the control of cell proliferation and in signal transduction. The regulation of CK2 is not well defined, and it has been considered a constitutively non-regulated protein kinase. We show that CK2 activation occurred during the progression of cell cycle in response to FBS stimuli of Go arrested cells. Importantly, we show that as the downstream target for CK2, the phosphorylation of eukaryotic translation-initiation factor eIF5 by CK2 may play a critical role in cell-cycle progression. Expression of eIF5 mutants that lack those phosphorylation sites by CK2 reveals that these mutants have a dominant-negative effect on phosphorylation of endogenous eIF5, as well as a significant reduction in the formation of the mature complex, the growth rate, and the expression of cell cycle-regulated proteins. Also, a pool of CK2 translocates into the nuclear fraction following its activation during the progression of the cell cycle. We identified molecules associated with CK2 in the nuclear fraction, by mass spectrometric analysis and by antibody micro arrays. Consistent with these findings, we report that CK2 may be involved in the regulation of cell cycle progression through the phosphorylation of a key molecule for translation initiation and of nuclear substrates upon activation of CK2 by itself.

  • Research Products

    (14 results)

All 2008 2007 2006

All Journal Article (8 results) (of which Peer Reviewed: 3 results) Presentation (6 results)

  • [Journal Article] Cell cycle and activation of CK22008

    • Author(s)
      Homma, MK, et. al.
    • Journal Title

      Mol.Cell.Biochem. (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Cell cycle and activation of CK22008

    • Author(s)
      Homma, MK, et. al.
    • Journal Title

      Mol Cell Biochem (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Involvement of thioredoxin reduetase 1 in the regulation of redox balance and viability of rheumatoid aynovial cells2008

    • Author(s)
      Kabuyama, Y, et. al.
    • Journal Title

      Bioehem Biophys Res Commu (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Enhancement of lymphocyte migration and cytokine production by ephrinB1 system in rheumatoid arthritis2007

    • Author(s)
      Kitamura T, et. al.
    • Journal Title

      Am J Physiol Cell Physiol 294

      Pages: C189-C196

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Involvemnet of selenoprotein P in the regulation of redox balance and myofibroblast viability in idiopathic pulmonary fibrosis2007

    • Author(s)
      Kabuyama Y, et. al.
    • Journal Title

      Genes Cells 12

      Pages: 1235-1244

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Enhancement of lymphocyte migration and cytokine production by ephrin B1 system in RA.2007

    • Author(s)
      Kitamura, T, et. al.
    • Journal Title

      Am J. Phys Cell Physiol 294

      Pages: C189-196

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Involvement of selenoprotein P in the regulation of redox bafance and Myofibroblast viability in idiopathic pulmonary fibrosis.2007

    • Journal Title

      Genes Cells 12

      Pages: 1235-1244

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Involovinent of ephrinBl R/ephrinB2 in chronic neuropathic pain.2007

    • Journal Title

      Spine 32

      Pages: 1592-1598

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Nuclear translocation of CK2 associated with progression of ceil cycle2007

    • Author(s)
      Homma, MK
    • Organizer
      BMB2007(第30回日本分子生物学会年会第80回日本生化学会大会合同大会)
    • Place of Presentation
      Yokohama JAPAN(Oral Presentation).
    • Year and Date
      2007-12-11
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Cell cycle regulated nuclear translocation of protein kinase CK22007

    • Author(s)
      Homma, MK, et. al.
    • Organizer
      5th International Conference on Protein Kinase CK2, Protein kinase CK2 and Disease.(Oral Presentation)
    • Place of Presentation
      Padova ITALY
    • Year and Date
      2007-09-15
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Cell cycle regulated nuclear translocation of protein kinase CK22007

    • Author(s)
      Homma, MK
    • Organizer
      5th internationla Conference on Protein Kinase CK2, Protein kinase CK2 and Disease.
    • Place of Presentation
      Padova ITALY(Oral Presentation)
    • Year and Date
      2007-09-15
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Inhibition of CK2 activity by adenomatous poiyposis coli protein2007

    • Author(s)
      Homma, K
    • Organizer
      Cold Spring Harbor Laboratory Meeting on Phosphorylation, Signaling and Disease.
    • Place of Presentation
      NY USA.(Oral Presentation)
    • Year and Date
      2007-05-19
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Cell Cycle-regulated phosphorylation and nuclear translocation of Protien kinase CK22006

    • Author(s)
      Homma, K
    • Organizer
      20th lUBMB Internationla Congress of Biochemistrv and Molecular Bioloav.
    • Place of Presentation
      Kyoto JAPAN
    • Year and Date
      2006-06-13
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Cell cycle regulated phosphorylation and nuclear localization of Protein kinase CK2.2006

    • Author(s)
      Homma, K
    • Organizer
      Cold Spring Harbor Laboratory Meeting on Cell Cycle.
    • Place of Presentation
      NY USA
    • Year and Date
      2006-05-18
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-02-04  

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