2007 Fiscal Year Final Research Report Summary
Proton circuit in mammalian system
| Project/Area Number |
18570140
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Doshisha Women's College of Liberal Arts |
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Principal Investigator |
WADA Ge-Hong Sun (和田 戈虹) Doshisha Women's College of Liberal Arts, Faculty of Pharmaceutical Science, Associate Professor (00314427)
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| Project Period (FY) |
2006 – 2007
|
| Keywords | Small GTPase / Proton pump / Acidification / V-ATPase / pH sensor / Membrane traffick |
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| Research Abstract |
The small GTPase Arf6 and ARNO are involved in regulation of endocytosis. We demonstrated the crucial role of acidification of early endosomes and V-ATPase/ARNO/Arf6 interactions in the regulation of the degradative pathway during endocytosis. We also found that V-ATPase with the α3 isoform is highly expressed in pancreatic islet, and localized to membranes of insulin-containing secretory granules in b cells. oc/oc mice, which have a null mutation at the α3 locus, exhibited a reduced level of insulin in the blood, even with high glucose administration. These results suggest that the α3 isoform of V-ATPase has regulatory functions in ,exocytosis of insulin secretion.
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