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2007 Fiscal Year Final Research Report Summary

Molecular mechanism of transcriptional regulation of IL-6 gene by heat shock transcription factor

Research Project

Project/Area Number 18570162
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionYamaguchi University

Principal Investigator

INOUE Sachie  Yamaguchi University, Graduate School of Medicine, Associate Professor (60159978)

Project Period (FY) 2006 – 2007
Keywordsgene / transcriptional regulation / cytokines / stress response / immune response / inflammatory response / genetic information / heat shock response
Research Abstract

Heat shock transcription factor 1 (HSF1) not only regulates expression of heat shock genes in response to elevated temperature, but is also involved in developmental processes by regulating genes such as cytokine genes. However, we did not know molecular mechanisms how HSF1 regulates non-heat shock genes. Here, we showed that constitutive HSF1 binding to IL-6 promoter is necessary for its maximal induction by LPS-stimulation in mouse embryo fibroblasts and peritoneal macrophages. Lack of HSF1 inhibited LPS-induced in vivo binding of an activator NF-kB and a repressor ATF3 to IL-6 promoter. Neither NF-kB nor ATF3 binds to IL-6 promoter in unstimulated HSF1-null cells even if they were overexpressed. Treatment with histone deacetylase inhibitor or a DNA methylation inhibitor restored LPS-induced IL-6 expression in HSF1-null cells, and histone modification enzymes were recruited on IL-6 promoter in the presence of HSF1. Consistently, chromatin structure of IL-6 promoter in the presence of HSF1 was more opened than that in its absence. These results indicate that HSF1 partially opens chromatin structure of IL-6 promoter for an activator or a repressor to bind to it, and provides a novel mechanism of gene regulation by HSF1

  • Research Products

    (19 results)

All 2008 2007 Other

All Journal Article (6 results) (of which Peer Reviewed: 3 results) Presentation (12 results) Remarks (1 results)

  • [Journal Article] Attenuation of progressive hearing loss in a model of age-related hearing loss by a heat shock protein inducer, Geranylgeranylacetone2008

    • Author(s)
      Takefumi Mikuriya, et. al.
    • Journal Title

      Brain research (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Attenuation of progressive hearing loss in a model of age-related hearing loss by a heat shock protein inducer. Geranylgeranylacetone2008

    • Author(s)
      T. Mikuriya, et. al.
    • Journal Title

      Brain research (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Heat shock transcription factor 1 is required for maintenance of ciliary beating in mice.2007

    • Author(s)
      Takaki Eiichi, et. al.,
    • Journal Title

      The Journal of Biological Chemistry 282

      Pages: 37285-37292

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Heat shock transcription factor 1 opens chromatin structure of interleukin-6 promoter to facilitate binding of an activator or a repressor.2007

    • Author(s)
      Inouye Sachiye, et. al.,
    • Journal Title

      The Journal of Biological Chemistry 282

      Pages: 33210-33217

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Heat shock factor 1 is required for maintenance of ciliary beating in mice2007

    • Author(s)
      E. Takaki, et. al.
    • Journal Title

      The Journal of Biological Chemistry 282

      Pages: 37285-37292

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Heat shock transcription factor 1 opens chromatin structure of interleukin-6 promoter to facilitate binding of an activator or a repressor2007

    • Author(s)
      S. Inouye, et. al.
    • Journal Title

      The Journal of Biological Chemistry 282

      Pages: 33210-33217

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] レンズ発生過程におけるHSF群による転写制御の機構2008

    • Author(s)
      藤本 充章 等
    • Organizer
      08'遺伝情報DECODE・冬のワークショップ
    • Place of Presentation
      越後湯沢
    • Year and Date
      20080121-23
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Transcriptional regulation by heat shock transcription factors during development of lends2008

    • Author(s)
      M. Fujimoto, et. al.
    • Organizer
      08 DECODE
    • Place of Presentation
      Echigoyuzawa
    • Year and Date
      20080121-23
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 熱ショック転写因子HSF1はIL-6遺伝子のクロマチンを開くことによって転写制御に関与する2007

    • Author(s)
      井上 幸江 等
    • Organizer
      第80回日本生化学会大会・第30回日本分子生物学会年会合同大会
    • Place of Presentation
      横浜
    • Year and Date
      20071211-15
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 熱ショック転写因子HSF4が認識するDNA結合配列の同定2007

    • Author(s)
      藤本 充章, 等
    • Organizer
      第80回日本生化学会大会・第30回日本分子生物学会年会合同大会
    • Place of Presentation
      横浜
    • Year and Date
      20071211-15
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 熱ショック転写因子HSF1のターゲット遺伝子の網羅的解析2007

    • Author(s)
      林田 直樹 等
    • Organizer
      第80回日本生化学会大会・第30回日本分子生物学会年会合同大会
    • Place of Presentation
      横浜
    • Year and Date
      20071211-15
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Heat shock transcription factor open chromatin structure of IL-6 promoter to regulate its expression2007

    • Author(s)
      S. Inoue, et. al.
    • Organizer
      BMB 2007
    • Place of Presentation
      Yokohama
    • Year and Date
      20071211-15
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Identification DNA sequence bound by heat shock transcription 42007

    • Author(s)
      M. Fujimoto, et. al.
    • Organizer
      BMB 2007
    • Place of Presentation
      Yokohama
    • Year and Date
      20071211-15
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Systemic analysis of target genes of heat shock transcription factor 12007

    • Author(s)
      N. Hayashida, et. al.
    • Organizer
      BMB 2007
    • Place of Presentation
      Yokohama
    • Year and Date
      20071211-15
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 熱ショック転写因子による繊毛運動の維持2007

    • Author(s)
      中井 彰, 等
    • Organizer
      大学共同利用機関法人・自然科学研究機構(生理学研究所)「上皮膜機能活性化物質と上皮膜防衛の最前線」
    • Place of Presentation
      岡崎
    • Year and Date
      20071101-02
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 熱ショック転写因子による細胞の生と死の決定機構2007

    • Author(s)
      林田 直樹, 等
    • Organizer
      大学共同利用機関法人・自然科学研究機構(生理学研究所)「上皮膜機能活性化物質と上皮膜防衛の最前線」
    • Place of Presentation
      岡崎
    • Year and Date
      20071101-02
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Characterization of the DNA-binding sites of HSF4 that constitutively forms a trimer.2007

    • Author(s)
      M. Fujimoto, et. al.,
    • Organizer
      Third International Congress on Stress responses in Biology and Medicine
    • Place of Presentation
      Budapest, Hungary
    • Year and Date
      20070923-26
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Characterization of the DNA-binding sites of HSF4 that constitutively forms a timer2007

    • Author(s)
      M. Fujimoto, et. al.
    • Organizer
      Third International Congress on Stress Responses in Biology and Medicine
    • Place of Presentation
      Budapest, Hangary
    • Year and Date
      20070923-26
    • Description
      「研究成果報告書概要(欧文)」より
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://ds22.cc.yamaguchi-u.ac.jp/~seika2/

URL: 

Published: 2010-02-04  

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