2007 Fiscal Year Final Research Report Summary
Roles of the Golgi apparatus in cell growth regulation
| Project/Area Number |
18570173
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Kanazawa University |
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Principal Investigator |
NAKAMURA Nobuhiro Kanazawa University, Graduate School of Natural SCience and Technology, Associate Professor (50294955)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Golgi apparatus / cell cycle / signal transduction / maintenance of structure / phosphorvlation |
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| Research Abstract |
GRASP65 is phosphorylated at S277 by cdc2/cyclin B and may participate in the regulation of mitotic entry. The role of GRASP65 S277 phosphorylation in cell cycle regulation was analyzed by two approaches. (1) Injection of recombinant GRASP65 G2A mutant, which cannot localize to the Golgi apparatus and accumulate in the cytoplasm, into the cells inhibited the phosphorylation of histone H3 in early prophase but not the centrosome separation. This result suggested that phosphorylation of histone H3 and centrosome separation are independently regulated at the onset of mitosis and S277 phosphorylation of GRASP65 may have some role in the regulation of those events. (2) Biochemical analyses revealed that Plk 1 binds to GRASP65 phosphorylated by mitotic e and this was dependent on the phosphorylation of S277. The binding is mediated by polo-box domain of Plk1. This result suggested that GRASP65 phosphorylated at S277 recruit Plk1 and function as a signaling scaffold at the onset of mitosis.
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[Journal Article] The Interaction of Two Tethering Factors, p115 and COG complex, is Required for Golgi Integrity2007
Author(s)
Sohda, M., Misumi, Y., Yoshimura, S., Nakamura, N., Fusano, T., Ogata, S., Sakisaka, S., Ikehara, Y
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Characterization of ArfGAP1 and FinGER7 /FinGER8 interaction by quantitative yeast two-hybrid analysis2007
Author(s)
Akhter, H., Hayashida, Y., Yoshida, Y., Ohkuma, S., Yamamoto, H., Nakamura, N
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Journal Title
J Juzen Med Soc 116
Pages: 137-142
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Functional analysis of human Yip1/Yif1 homologs2007
Author(s)
Yoshida, Y., Suzuki, K., Akhter, H., Ojima, Y., Kumamoto, S., Hayashida, Y., Nakamura, N
Organizer
30th Annual meeting for the Japan Society for Molecular Biology and 80th Annual meeting of Japan Society for Biochemistry
Year and Date
20071211-15
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Mytl is necessary for reassembly of the Golgi apparatus and the ER during telophase2006
Author(s)
Nakajima, H., Yonemura, S., Murata, M., Nakamura, N., Piwnica-Worms, H., Nishida, E
Organizer
59th Annual meeting for the Japan Society for Cell Biology
Place of Presentation
Fukuoka, Japan
Year and Date
20060528-30
Description
「研究成果報告書概要(欧文)」より
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[Presentation] FinGERS and FinGER7 form a complex and maintain the Golgi strucure2006
Author(s)
Suzuki, K., Tanimoto, K., Yamaguchi, Y., Yoshida, Y., Nakamura, N
Organizer
59th Annual meeting for the Japan Society for Cell Biology
Place of Presentation
Fukuoka, Japan
Year and Date
20060528-30
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Cell cycle regulation by FinGER3 and FinGER42006
Author(s)
Yoshida, Y., Tanimoto, K., Sakai, N., Suzuki, K., Nakamura, N
Organizer
59th Annual meeting for the Japan Society for Cell Biology
Place of Presentation
Fukuoka, Japan
Year and Date
2006-05-01
Description
「研究成果報告書概要(欧文)」より
