2007 Fiscal Year Final Research Report Summary
Research on the mechanism of tight jundan regulafion by using botulinum toxir complex
| Project/Area Number |
18570178
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Osaka University |
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Principal Investigator |
FUJINAGA Yukako Osaka University, Research Institute for Microbial Diseases, SA, Associate Professor (60252954)
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| Project Period (FY) |
2006 – 2007
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| Keywords | tight junction / epithelial cell / cell to cell function / glycoprotein / botulinum toxin |
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| Research Abstract |
The type B botulinum neurotoxin (BoNT) elicits flaccid paralysis and death in humans by intoxicating peripheral nerves after oral absorption. Here, we examine the function of the hemagglutinin (HA), a non-toxic component of the large 16S BoNT complex. We find that the HA acts in the intestine to disrupt epithelial barrier function by opening intercellular tight and adherens junctions. This allows transport of BoNT and other large solutes into the systemic circulation and explains how the type B BoNT complexes are efficiently absorbed. In vitro, HA appears to act on the epithelial cell via the basolateral membrane only, suggesting the possibility of another step in the absorptive process. These studies show that the 16S BoNT complex is a multi-functional protein assembly equipped with the machinery to efficiently breach the intestinal barrier and act systemically on peripheral nerves.
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[Presentation] Transepithelial transport of the botulinum toxin via trans cellular and paracellular routes2007
Author(s)
Fujinaga, Y., Matsumura, T., Jin, Y., Takegahara, Y., Sugawara, Y
Organizer
IntemationalSymposium "Membranetraffic"Awaji-shima
Place of Presentation
Hyogo,Japan
Year and Date
20071127-29
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Disruption of the intestinal paracellular barrier by botulinum toxin complex2007
Author(s)
Matsumura, T., Jin, Y., Kabumoto, Y., Takegahara, Y., Sugawara, Y., Oguma, K., Lencer, WI., Fujinaga, Y
Organizer
The 7th Awaji International Forum on Infection and Immunity
Place of Presentation
Hyogo, Japan
Year and Date
20070901-05
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Lencer WI, and Fujinaga Y, The HA proteins of botulinum toxin induce paracellular leakage in intestinal epithelial cells to increase toxin absorption2007
Author(s)
Matsumura, T., Jin, Y., Kabumoto, Y., Takegahara, Y., Oguma, K
Organizer
ETOX 13 European Workshop on Bacterial Protein Toxins
Place of Presentation
San Martino al Cimino, Italy
Year and Date
20070623-28
Description
「研究成果報告書概要(欧文)」より
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