2007 Fiscal Year Final Research Report Summary
Study on the novel S-phase specific proteolysis pathway
| Project/Area Number |
18570181
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Cell biology
|
|---|
| Research Institution | University of Hyogo (2007)
Kyushu University (2006) |
|---|
Principal Investigator |
NISHITANI Hideo University of Hyogo, Grad. Sch. of Life Sci, Professor (40253455)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Keywords | Cell Cycle / Aroteolvsis / replication / ubiauitin / PCNA |
|---|
| Research Abstract |
Cdt1, an essential factor for DNA replication licensing, is degraded soon after entry into S-phase. We discovered that two ubiquitin ligases, Skp2-Cul1 and DDB1-Cul4, independently recognize the N-terminus of Cdt1 for ubiquitination. The first 10 amino acid region of Cdt1 contains a conserved sequence, PCNA interacting motif (PIP-box). During S-phase, PCNA and DDB1-Cul4 co-operate to ubiquitinate Cdt1. This system also operates after DNA damage, such as UV irradiation. When PIP-box was mutated, Cdt1 became stable after UV irradiation, and during S-phase combined with silencing of Skp2. Many PCNA interacting proteins were isolated. Among them, we found that CDK inhibitor p21 is also degraded through PCNA and DDB1-Cul4 system. Though both Cdt1 and p21 play key roles during G1-phase, their presence after initiation of S-phase is not suitable for proper cell cycle progression., and thus should be degraded upon entry into S-phase. We propose that PCNA dependent DDB1-Cul4 ubiquitin system provides a feedback system that helps the coordination between end of G1-phase and initiation of S-phase.
|
-
-
-
-
-
[Journal Article] Cdtl associates dynamically with chromatin throughout GI and recruits Geminin onto chromatin2007
Author(s)
Xouri G, Squire A, Dimaki M, Geverts B, Verveer, PJ, Taraviras S, Nishitani H, Houtsmuller, AB, Bastiaens, PI, Lygerou, Z
-
Journal Title
EMBO J 26(5)
Pages: 1303-1314
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Licensing regulators Geminin and Cdt1 identify progenitor cells of the mouse CNS in a specific phase of the cell cycle2007
Author(s)
Spella M, Britz O, Kotantaki P, Lygerou Z, Nishitani H, Ramsay, RG, Flordellis C, Guillemot F, Mantamadiotis T, Taraviras, S
-
Journal Title
Neuroscience 147(2)
Pages: 373-387
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
[Journal Article] Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis2006
Author(s)
Nishitani H, Sugimoto N, Roukos V, Nakanishi Y, Saijo M, Obuse C, Tsurimoto T, Nakayama, KI, Nakayama K, Fujita M, Lygerou Z, Nishimoto, T
-
Journal Title
EMBO J 25
Pages: 1126-1136
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
[Presentation] Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdtl for proteolysis2006
Author(s)
Nishitani H, Sugimoto N, Roukos V, Nakanishi Y, Saijo M, Obuse C, Tsurimoto T, Fujit M, Lygerou Z, Nishimoto T
Organizer
IUBMB
Place of Presentation
Kyoto
Year and Date
2006-06-23
Description
「研究成果報告書概要(欧文)」より
-
