2007 Fiscal Year Final Research Report Summary
Functional analysis of SUMO modification in nuclear architecture and transcription regulation
| Project/Area Number |
18570182
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Kumamoto University |
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Principal Investigator |
SAITOH Noriko Kumamoto University, Institute of Molecular Embryology and Genetics, Department of Regeneration Medicine, Assistant Professor (40398235)
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| Project Period (FY) |
2006 – 2007
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| Keywords | SUMO / in situ SUMOylation assay / PML body / Nuclear Speckle / RanBP2 |
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| Research Abstract |
In the cell nucleus, many important events including gene transcription, replication, repairs occur under complicated regulations. Nuclear factors are not randomly or evenly distributed within the nucleus, rather they are highly and dynamically compartmentalized into several domains, which enables many reactions to be rapid and efficient in the nucleus. Goal of this project is to elucidate the mechanism of how SUMOylation regulates the nucleus. I designed and performed following experiments.
SUMOylation by RanBP2 plays a role in nuclear domain formations.
With in situ SUMOylation assay that visualizes sub-cellular localization of SUMOylation activity, I showed that RanBP2 exerts SUMO E3 activity at the nuclear pore complex. By knockdown of RanBP2 by siRNA, SUMOylation activity at the nuclear pore complex was lost, together with several changes in nuclear domains, such as reduction of numbers of PML bodies and aberrant forms of nuclear speckles. A novel model was suggested, in which proteins are SUMOlyated at the nuclear pore complex by RanBP2, when they are transported from cytoplasm to the nucleus.
Function of nuclear speckle and the mechanism for its formation.
Nuclear speckle contains many factors for transcription, RNA processing, transport and so on and thus it is involved in gene expression regulation. I analyzed changes in gene expression patterns and localizations of nuclear speckle components in RanBP2 knockdown cells in which nuclear speckles are aberrantly formed. The study presents one of the first molecularly tractable pathways for establishing nuclear architecture. In summary, I revealed significant role of SUMOylation in nuclear domain formations, and made contributions to understand function and dynamics of the cell nucleus.
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[Journal Article] High mobility group protein HMGA1 inhibits retinoblastoma protein-mediated cellular GO arrest2007
Author(s)
Ueda, Y., Watanabe, S., Tei, S., Saitoh, N., Kuratsu, J. I., Nakao, M
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Journal Title
Cancer Sci 98
Pages: 1893-1901
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] 「研究成果報告書概要(欧文)」より2007
Author(s)
Tei, S., Sakamoto, Y., Akaboshi, S., Mishiro, T., Watanabe, S., Saitoh, N., Nakao, M
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Journal Title
Kourei Syoukika Igakukai 8/9
Pages: 28-37
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[Journal Article] chromatin reorganization in the MHC-Oct3/4 locus at developmental phases of embryonic stem cell differentiation2006
Author(s)
Aoto, T., Saitoh, N., Ichimura, T., Niwa, H., Nakao, M., Nuclear, M
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Journal Title
Dev. Biol 208
Pages: 354-367
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] In situ SUMVIOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies2006
Author(s)
Saitoh, N., Uchimura, U., Tachibana, T., Sugahara, S., Saitoh, H., Nakao, M
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Journal Title
Exp. Cell Res 312
Pages: 1418-1430
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Nuclear organelles in the interchromosomal region2006
Author(s)
Saitoh, N., Aoto, T., Tei, S., Niki, E., Nakatsu, Y., Nakao, M
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Journal Title
Tanpakushitsu Kakusan Koso 51
Pages: 1957-1963
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Nuclear domain involved in gene expression regulation2008
Author(s)
N., Saitoh, Y., Nakatsu, K., Goshi, Y., Taniguchi, T., Funahara, M., Nakao
Organizer
24th Chromosome workshop
Place of Presentation
Atami, Japan
Year and Date
2008-01-30
Description
「研究成果報告書概要(欧文)」より
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[Presentation] SUMO ylaSUMOylationtion in formation of sub-nuclear structures2007
Author(s)
Saitoh, N., Nakatsu, Y., Hino, Y., Uchimura. Y., Saitoh, H., Nakao, M.
Organizer
EIVIBO Conference Series on Nuclear Structure and Dynamics
Place of Presentation
Montpellier, France
Year and Date
2007-09-03
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Nuclear architecture and SUMO modification2007
Author(s)
Saitoh, N., Nakatsu, Y., Hino Y., Uchimura, Y., Saitoh, H., M., Nakao
Organizer
Joint Meeting of the Japanese Society of Developmental Biologists and the Japan Society for Cell Biology
Place of Presentation
Fukuoka, Japan
Year and Date
2007-05-28
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 核内構造体の形成機構2007
Author(s)
斉藤 典子
Organizer
第24回染色体ワークショソプ
Place of Presentation
唐津市
Year and Date
2007-01-31
Description
「研究成果報告書概要(和文)」より
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[Presentation] A mechanism for nuclear arcitecture formation2007
Author(s)
Saitoh, N., Nakatsu, Y., Uchimura, Y., Saitoh, H., Nakao, M.
Organizer
24th Chromosome work shop
Place of Presentation
Karatsu, Japan
Year and Date
2007-01-31
Description
「研究成果報告書概要(欧文)」より
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[Presentation] SUMO pathway plays a role in nuclear organization2006
Author(s)
Saitoh, N., Uchimura, Y., Tachibana, T., Sugahara, S., H., Saitoh, M., Nakao
Organizer
20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
Place of Presentation
Kyoto, Japan
Year and Date
2006-06-23
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Nucelar regulation during mouse cell differentiation2006
Author(s)
Aoto, T., Saitoh, N., Ichimura, T., Niwa, H., Nakao, M.
Organizer
6th Nuclear Dynamics
Place of Presentation
Mashiki, Japan
Year and Date
2006-05-17
Description
「研究成果報告書概要(欧文)」より
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[Presentation] A role of SUMO ylation in formation of nuclear sub-structure2006
Author(s)
Saitoh, N., Uchimura, Y., Sugahara, S., Tachibana, T., Saitoh, H., Nakao, M.
Organizer
the 6th Nuclear Dynamics
Place of Presentation
Mashiki, Japan
Year and Date
2006-05-17
Description
「研究成果報告書概要(欧文)」より
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[Presentation] SUMO E3 Ligase RanBP2 plays a role in formation of sub-nuclear structures2006
Author(s)
Saitoh, N., Uchimura, Y., Aoto, T., Saitoh, H., Nakao, M.
Organizer
EMBO Workshop, Functional Organization of the Cell Nucleus
Place of Presentation
Prague, Czech Republic
Year and Date
2006-05-05
Description
「研究成果報告書概要(欧文)」より
