2007 Fiscal Year Final Research Report Summary
Functional analysis of cnil ; an intra-cellular transporter of EGF-repeat-containing growth factors
| Project/Area Number |
18570195
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Kyoto University |
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Principal Investigator |
CHISAKA Osamu Kyoto University, Graduate Schcol of Biostudies, Associate Professor (80188474)
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| Project Period (FY) |
2006 – 2007
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| Keywords | cornichon-like protein / cornichon homolog 2 / neural crest cells / cranial nerve / HB-EGF / ErbB4 / secretion / migration |
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| Research Abstract |
During their migration to the periphery, cranial neural crest cells (NCCs) are repulsed by an ErbB4-dependent cue(s) in the mesenchyme adjoining rhombomeres (r) 3 and 5, which are segmented hindbrain neuromeres. ErbB4 has many ligands, but which ligand functions in the above system has not yet been clearly determined. Here we found that a cornichon-like protein/cornichon homolog 2 (CNIL/CNIH2) gene was expressed in the developing chick r3 and r5. In a cell culture system, its product facilitated the secretion of heparin-binding epidermal growth factor-like growth factor (HB-EGF), one of the ligands of ErbB4. When CNIL function was perturbed in chick embryos by forced expression of a truncated form of CNIL, the distribution of NCCs was affected, which resulted in abnormal nerve fiber connections among the cranial, sensory ganglia. Also, knockdown of CNIL or HB-EGF with siRNAs yielded a similar phenotype. This phenotype closely resembled that of ErbB4 knockout (KO) mouse embryos. Since HB-EGF was uniformly expressed in the embryonic hindbrain, CNIL seems to confine the site of HB-EGF action to r3 and r5 in concert with ErbB4.
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[Journal Article] Cornichon-like Protein Facilitates Secretion of HB-EGF and Regulates Proper Development of Cranial Nerves2007
Author(s)
Hideharu, Hoshino, Tsukasa, Uchida, Toshiaki. Otsuki, Shoko. Kawamoto, Kousaku, Okubo, Masatoshi, Takeichi, Osamu, Chisaka
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Journal Title
Molecular Biology of the Cell Vol.18
Pages: 1143-1152
Description
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