2007 Fiscal Year Final Research Report Summary
Role of membrane trafficking in Wnt signaling that regulates gastrulation
| Project/Area Number |
18570212
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
KINOSHITA Noriyuki National Institute for Basic Biology, Department of Developmental Biology, Associated Professor (30300940)
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| Project Period (FY) |
2006 – 2007
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| Keywords | gastrulation / membrane trafficking / Wnt signaling |
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| Research Abstract |
Rab GTPases are key regulators of intracellular membrane trafficking. We sought to elucidate the roles of Rab GTPases in Xenopus gastrulation, and found that a Xenopus homolog of Rab40 (XRab40) is required for normal gastrulation. XRab40 is localized at the Golgi apparatus and interacts with ElonginB/C and Cullin5 to form a ubiquitin ligase. XRab40/XCullin5 functions cooperatively and regulates the ubiquitination and localization of Rap2 GTPase. Furthermore, XRab40/XCullin5 regulates the membrane localization of Dishevelled, a key signaling molecule in the Wnt pathway, through Rap2 and its effector Misshapen/Nck interacting kinase (XMINK). XMINK interacts with Dishevelled, and is translocated to the plasma membrane by Wnt activation. We propose a novel signaling cascade consisting of XRab40/XCullin5, Rap2 and XMINK, which plays a crucial role in the regulation of the noncanonical Wnt pathway.
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