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2007 Fiscal Year Final Research Report Summary

Analyses of intercellular interaction and genome structure change during heat tolerant evolution by using experimental evolution system with Escherichia coli

Research Project

Project/Area Number 18570219
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Evolutionary biology
Research InstitutionToho University

Principal Investigator

KISHIMOTO Toshihiko  Toho University, Faculty of Science, Associate Professor (90339200)

Co-Investigator(Kenkyū-buntansha) YOMO Tetsuya  OSAKA UNIVERSITY, Graduate School of Information Science and Technology, Professor (00222399)
Project Period (FY) 2006 – 2007
KeywordsEscherichia coli / Heat Tolerant Evolution / Quorum Sensing / Diversity
Research Abstract

We have researched about the dynamics both of cellular and E. coli population level during adaptation to heat stress (high temperature; above 39 degree). First, we constructed the serially transfer culture system maintaining specific growth rate, then transferred E. coli culture to high temperature (2 degree above past temperature). We could evolve E. coli to accept 45 degree condition that was highest temperature E. coli could survive in minimum conditioned medium. During 39 degree adaptation, just after temperature transition, growth rate decreased rapidly and standard deviation of fluorescence of cellular green fluorescence protein (GFP) of E. coli population enhanced in 2~7days, then growth rate recovered and SD of GFP was decreased for 10-20 days and converged to 39 degree specific values. These results indicated that temperature shift induced diversity of intracellular conditions that might bring the recovery of plasticity of E. coli population, and then adaptation was facilitated by E. coli diversity. At the early stage of 45 degree adaptation, E. coli could grow cell density dependent-manner. This dependency was derived from secreted factors from E. coli, therefore, temperature shift might induce quorum sensing. We have been searching corresponding secreted factors from E. coli culture supernatant. Moreover, average genome DNA content in E. coli cell increased in proportion to adaptation to higher temperature, and interestingly content both of guanine and cytosine decreased according to adaptation to higher temperature. Our results suggested that heat tolerant adaptation and evolution of E. coli might be brought by diversification and/or interaction among E. roll population, and genome DNA content increment and G/C content decrement were observed through fixation process of evolved phenotype.

  • Research Products

    (10 results)

All 2007 2006

All Journal Article (4 results) (of which Peer Reviewed: 2 results) Presentation (6 results)

  • [Journal Article] Regulation of CD8+ T Cell Development by Thymus-Specific Proteasomes2007

    • Author(s)
      Shigeo Murata
    • Journal Title

      Science 316

      Pages: 1349-1353

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Regulation of CD8+ T Cell Development by Thymus-Specific Proteasomes2007

    • Author(s)
      Shigeo, Murata
    • Journal Title

      Science 316

      Pages: 1349-1353

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cooperation of multiple chaperones required for the assembly of mammalian 20S proteasomes2006

    • Author(s)
      Yuko Hirano
    • Journal Title

      Molecular Cell 24

      Pages: 977-984

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Cooperation of multiple chaperones required for the assembly of mammalian 20S proteasomes2006

    • Author(s)
      Yuko, Hirano
    • Journal Title

      Mol. Cell 24(6)

      Pages: 977-984

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 39℃適応によるブィットネスと適応進化と揺らぎの関係2007

    • Author(s)
      岸本 利彦
    • Organizer
      日本進化学会第9回大会
    • Place of Presentation
      京都大学
    • Year and Date
      2007-09-01
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 高温適応と大腸菌の経験温度履歴の相関2007

    • Author(s)
      辰巳 誠
    • Organizer
      日本進化学会第9回大会
    • Place of Presentation
      京都大学
    • Year and Date
      2007-09-01
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Dynamics of fluctuation'bf protein contents correlated with adaptation to 39 degree2007

    • Author(s)
      Toshihiko, Kishimoto
    • Organizer
      The 9th annual meeting in Kyoto, Sciety of Evolutionary Studies, Japan
    • Place of Presentation
      Kyoto University
    • Year and Date
      2007-09-01
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Relationship between adaptation to heat stress and genetic background of Escherichia coli2007

    • Author(s)
      Makoto, Tatsumi
    • Organizer
      The 9th annual meeting in Kyoto, Sciety of Evolutionary Studies, Japan
    • Place of Presentation
      Kyoto University
    • Year and Date
      2007-09-01
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Adaptation dynamics for high temperature and heat tolerant evolution of Escherichia coli2006

    • Author(s)
      大久保 将人
    • Organizer
      EABS&BSJ2006
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2006-11-15
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Adaptation dynamics for high temperature and heat tolerant evolution of Escherichia coli2006

    • Author(s)
      Masato, Okubo
    • Organizer
      EABS & BSJ 2006
    • Place of Presentation
      Okinawa Convention Center
    • Year and Date
      2006-11-15
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-02-04  

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