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2007 Fiscal Year Final Research Report Summary

REGULATION OF TUBEROUS ROOT FORMATION BY CDK-ACTIVATING KINASE IN SWEET POTATO

Research Project

Project/Area Number 18580016
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Crop science/Weed science
Research InstitutionKyushu University

Principal Investigator

SAITOU Kazuyuki  Kyushu University, Faculty of Agriculture, Associate Professor (00215534)

Project Period (FY) 2006 – 2007
KeywordsSWEET POTATO / TUBEROUS ROOT FORMATION / CDK-ACTIVATTNG KINASE / GENE EXPRESSION
Research Abstract

Two cDNAs for a CDK-activating kinase and a cyclin H were cloned and designated Ipoba; CDKD; 1 and Ipoba; CycH; 1, respectively. The deduced amino acid sequences for Ipoba; CDKD; 1 contained an ATP-binding site, an kinase active-site and the conserved threonine residue within the T-loop. The deduced amino acid sequences for Ipoba; CycH; 1 contained N-terminal domain. Ipoba; CDKD; 1 transcript was detected in tuberous roots with the lowest level, although the gene was expressed in allorgans. There was little variety found among the relative abundance of transcripts in different organs. The levels of sucrose, ABA and cytokinins increase in the process of tuberous root formation. Therefore, the effect of these signal molecules on the expression of both Ipoba; CDKD; 1 and Ipoba; CycH; 1. The expression of Ipoba; CDKD; 1 increased by application of sucrose solution, while the expression of Ipoba; CycH; 1 was not significantly affected by sucrose. ABA induced the expression of Ipoba; CDKD; 1, whereas the expression of Ipoba; CycH; 1 was not influenced by ABA. The relative amount of Ipoba; CDKD; 1 mRNA was reduced by benzyladenine, while benzyladenine increased the relative amount of Ipoba; CycH; 1 mRNA. Sucrose antagonized the inhibitory effect of benzyladenine for Ipoba; CDKD; 1 gene expression. Benzyladenine enhanced the expression of Ipoba; CycH; 1 when sucrose was supplemented. These results suggested that cell proliferation was controlled via regulation of CDK-activating kinase and cyclin H gene expression by alterations in the concentration of sucrose, ABA and cytokinin.

  • Research Products

    (24 results)

All 2008 2007 2006

All Journal Article (12 results) (of which Peer Reviewed: 1 results) Presentation (12 results)

  • [Journal Article] Response of growth,gas exchange and PSII electron transport in green-gram(Vigna radiata L., Wilczed)varieties and other pulse species to drought and re-watering2008

    • Author(s)
      Oo, H.H., et. al.
    • Journal Title

      Journal of the Faculty of Agriculture Kyushu University 53

      Pages: 19-25

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Response of growth, gas exchange and PSII electron transport in green-gram(Vigna radiata L., Wilczek)varieties and other pulse species to drought and re-watering2008

    • Author(s)
      H.H., Oo
    • Journal Title

      JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY NOT REVIEWED 53

      Pages: 19-25

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] サツマイモにおけるADPグルコースピロボスホリラーゼ遺伝子のプロモーター解析2007

    • Author(s)
      小橋 章子, ら
    • Journal Title

      日本作物学会紀事 76別号2

      Pages: 298-299

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 窒素によるイネRubisco小サブユニット遺伝子の発現調節2007

    • Author(s)
      宮碕 信朗, ら
    • Journal Title

      日本作物学会紀事 76別号2

      Pages: 118-119

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Dof転写因子を介した窒素によるイネRubisoo小サブユニット遺伝子の発現制御機構2007

    • Author(s)
      宮崎 信朗, ら
    • Journal Title

      日本作物学会紀事 76別号2

      Pages: 120-121

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Promoter analysis of an ADP-glucose pyrophosphorylase gene, ibAGP1, in sweet potato(Ipomoea batatas Lam.)2007

    • Author(s)
      A., Kobashi
    • Journal Title

      JAPANESE JOURNAL OF CROP SCIENCE, NOT REVIEWED 76

      Pages: 298-299

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression regulation of genes for Rubisco small subunits in response to nitrogen supply in rice2007

    • Author(s)
      N., Miyazaki
    • Journal Title

      JAPANESE JOURNAL OF CROP SCIENCE, NOT REVIEWED 76

      Pages: 118-119

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Roles of Dof transcription factors in nitrogen-regulated gene expression of Rubisco small subunits in rice2007

    • Author(s)
      N., Miyazaki
    • Journal Title

      JAPANESE JOURNAL OF CROP SCIENCE, NOT REVIEWED 76

      Pages: 120-121

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] サツマイモに存在するサイクリンH:遺伝子の発現解析2006

    • Author(s)
      永田 敬文, ら
    • Journal Title

      日本作物学会紀事 75別号2

      Pages: 272-273

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Increase in the expression of an alpha-amylase gene and sugar accumulation induced during cold period reflects shoot elongation in hyacinth bulbs2006

    • Author(s)
      Satoh, A., et. al.
    • Journal Title

      Journal of the American society for horticulture science 131

      Pages: 185-191

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Expression analysis of a gene for a cyclin H in sweet potato (Ipomoea batatas Lam.)2006

    • Author(s)
      T., Nagata
    • Journal Title

      JAPANESE JOURNAL OF CROP SCIENCE, NOT REVIEWED 76

      Pages: 272-273

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Increase in the expression of an alpha-amylase gene and sugar accumulation induced during cold period reflects shoot elongation in hyacinth bulbs2006

    • Author(s)
      A., Satoh
    • Journal Title

      JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURE SCIENC, REVIEWED 131

      Pages: 185-191

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] The rice OsDof-24 gene encoding a Dof transcription factor regulates gene expression of Rubisco small subunits in response to nitrogen supply2008

    • Author(s)
      Miyazaki, N, et. al.
    • Organizer
      The 5th international crop science congress
    • Place of Presentation
      韓国チェジュ
    • Year and Date
      20080414-15
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] The rice OsDof-24 gene encoding a Dof transcription factor regulates gene expression of Rubisco small subunits in response to nitrogen supply2008

    • Author(s)
      N., Miyazaki
    • Organizer
      THE 5TH INTERNATIONAL CROP SCIENCE CONGRESS
    • Place of Presentation
      JEJU, KOREA
    • Year and Date
      20080414-15
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] サツマイモにおけるADPグルコースピロボスホリラーゼ遺伝子のプロモーター解析2007

    • Author(s)
      小橋 章子, ら
    • Organizer
      日本作物学会
    • Place of Presentation
      石川
    • Year and Date
      2007-09-27
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Promoter analysis of an ADP-glucose pyrophosphorylase gene, ibAGP1, in sweet potato(Ipomoea batatas Lam.)2007

    • Author(s)
      A., Kobashi
    • Organizer
      CROP SCIENCE SOCIETY OF JAPAN
    • Place of Presentation
      ISHIKAWA
    • Year and Date
      2007-09-27
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] サツマイモに存在するサイクリンH遺伝子の発現解析2006

    • Author(s)
      永田 敬文, ら
    • Organizer
      日本作物学会
    • Place of Presentation
      香川
    • Year and Date
      2006-10-29
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Expression analysis of a gene for a cyclin H in sweet potato expression of Rubisco small subunits in rice2006

    • Author(s)
      T., Nagata
    • Organizer
      CROP SCIENCE SOCIETY OF JAPAN
    • Place of Presentation
      KAGAWA
    • Year and Date
      2006-10-29
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Regulation of cell division activity and sink activity by sucrose and cytokinin during tuberous root formation of sweet potato(Ipomoea batatas Lam.)2006

    • Author(s)
      T., Nagata
    • Organizer
      20TH IUBMB INTERNATIONAL CONGRESS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY AND 11TH FAOBMB CONGRESS
    • Place of Presentation
      KYOTO
    • Year and Date
      2006-07-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Regulation of gene expression of sedo-heptulose-1, 7-bis-Phosphatase and chloroplastic fructose-1, 6-bisphosphatase by nitrogen in rice leaf2006

    • Author(s)
      A., Yamasaki
    • Organizer
      20TH IUBMB INTERNATIONAL CONGRESS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY AND 11TH FAOBMB CONGRESS
    • Place of Presentation
      KYOTO
    • Year and Date
      2006-07-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Expression analysis of the ribulose-1, 5-bisphosphate carboxylase/oxygenase gene family in rice(Oryza sativa L.)2006

    • Author(s)
      N., Miyazaki
    • Organizer
      20TH IUBMB INTERNATIONAL CONGRESS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY AND 11TH FAOBMB CONGRESS
    • Place of Presentation
      KYOTO
    • Year and Date
      2006-07-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Regulation of gene expression of sedoheptulose-1,7-bisphosphatase and chloroplastic fructose-1,6-bisphosphatase by nitrogen in rice leaf2006

    • Author(s)
      Yamasaki, A., et. al.
    • Organizer
      20th IUBMB international congress of biochemistry and molecular biology and 11th FAOBMB congress
    • Place of Presentation
      京都
    • Year and Date
      2006-06-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Expression analysis of the ribulose-1,5-bisphosphate carboxylase/oxygenase gene family in rice (Oryza satia L.)2006

    • Author(s)
      Miyazaki, N., et. al.
    • Organizer
      20th IUBMB international congress of biochemistry and molecular biology and 11th FAOBMB congress
    • Place of Presentation
      京都
    • Year and Date
      2006-06-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Regulation of cell division activity and sink activity by sucrose and cytokinin during tuberous root formation of sweet potato(lpomoea bata tas Lam.)2006

    • Author(s)
      Nagata, T., et. al.
    • Organizer
      20th IUBMB international congress of biochemistry and molecular biology and 11th FAOBMB congress
    • Place of Presentation
      京都
    • Year and Date
      2006-06-21
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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