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2007 Fiscal Year Final Research Report Summary

Mcroorgarisms composing synlrophic melhanoseric communly in paddy fieidsoil

Research Project

Project/Area Number 18580059
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionNagoya University

Principal Investigator

ASAKAWA Susumu  Nagoya University, graduate School of Bioagricultural Sciences, Associate Professor (50335014)

Project Period (FY) 2006 – 2007
KeywordsPaddy field soil / Methanoaenesis / Syntroohic microbial system / Cultivation method / Molecular ecology / Organic acid / Inter-species hydrogen transfer / Denaturing gradient gel electrophoresis method
Research Abstract

The objective of the present research project was to characterize microorganisms composing syntrophic methanogenic community in paddy field soil by the cultivation method and molecular ecological techniques. Methanogenic cultures were enriched on propionate and acetate inoculated with paddy field soil and the microbial communities in the cultures were examined. A DNA fragment closely related to Proteiniphilum acetatigenes was obtained from the culture on propionate by the denaturing gradient gel electrophoresis method. P. acetatigenes alone does not degrade propionate nor produce methane, but stimulates degradation of propionate and methanogenesis when the bacterium coexists in the propionate oxidizing syntrophic culture. The results suggested that similar syntrophic interaction may have functioned in the paddy field soil. Methanosarcina- or Methanobacterium-like methanogenic archaea and a bacterium were predominated in the culture on acetate. A hydrogenotrophic methanogenic strain, Methanobacterium sp. AH1, and a sulfate reducer, Desulfovibrio sp. Al, were isolated from the enrichment culture. Co-culture of strains AH1 and A1 degraded pyruvate, lactate and ethanol to methane, indicating syntrophic association of hydrogenotrophic methanogens and sulfate reducers for methanogenesis in paddy field soil. However, the co-culture did not degrade acetate to methane and syntrophic acetate oxidation could not be demonstrated

  • Research Products

    (6 results)

All 2006 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (4 results)

  • [Journal Article] 水田土壌の機能と微生物群集構造2006

    • Author(s)
      浅川 晋
    • Journal Title

      土と微生物 60

      Pages: 79-84

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Community structure of functional microorganisms in paddy field soil

    • Author(s)
      ASAKAWA, Susumu
    • Journal Title

      Soil Microorganisms

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Community structure of functional microorganisms In paddy field soil2006

    • Author(s)
      ASAKAWA, Susumu
    • Organizer
      The annual meeting, Japanese Society of Soil Microbiology
    • Place of Presentation
      Sendai
    • Year and Date
      2006-10-06
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 水田土壌を接種源とし酢酸を基質として集積したメタン生成共生系を構成する微生物群の解析2006

    • Author(s)
      森田麻友美, 渡邉健史, 木村眞人, 浅川 晋
    • Organizer
      日本土壌肥料学会2006年度秋田大会
    • Place of Presentation
      秋田県立大学
    • Year and Date
      2006-09-07
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Microorganisms composing syntrophic methanogenic einrichment culture on acetate inoculated with paddy field soil2006

    • Author(s)
      MORITA, Mayumi, WATANABE, Takeshi, KIMURA, Makoto, ASAKAWA, Susumu
    • Organizer
      The annual meeting, Japanese Society of Soil Science and Plant Nutrition
    • Place of Presentation
      Akita
    • Year and Date
      2006-07-09
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 水田土壌の機能と微生物群集構造2006

    • Author(s)
      浅川 晋
    • Organizer
      日本土壌微生物学会2006年度仙台大会
    • Place of Presentation
      東北大学
    • Year and Date
      2006-06-10
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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