2007 Fiscal Year Final Research Report Summary
Analysis on the physiological roles of phospholipases A_2
| Project/Area Number |
18580067
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ARIOKA Manabu The University of Tokyo, Graduate School of Agricultural and Life Sciences, Associate Professor (20242159)
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| Project Period (FY) |
2006 – 2007
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| Keywords | phospholipase A_2 / lysophospholipid / MAP kinase / PC12 / Aspergillus oryzae |
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| Research Abstract |
Nerve growth factor (NGF) induces MAP kinase activation and neuronal differentiation in PC12cells. We found that simultaneous addition of lysophospholipids, lysophosphatidylcholine (LPC) and sphingosylphosphorylcholine (SPC), markedly enhances NGF-induced MAP kinase activation, as examined by its phosphorylation, and neurite outgrowth. In agreement, the induction of immediate early gene expression by NGF was potentiated by LPC and SPC. Removal of extracellular Ca^<2+> did not show any effect. Studies using various inhibitors of suggested that the synergistic effect of NGF and LPC/SPC requires the activation of specific cellular signaling pathway(s).
Putative cytosolic phospholipase A_2 gene, AoplaA, uniquely found in Aspergillus oryzae genome was studied. Localization analysis using AoPlaA-EGFP fusion protein demonstrated that it localizes to mitochondria, which was supported by the biochemical fractionation experiment. To examine whether the N-terminal region contains the mitochondrial targeting signal, EGFP-fusion proteins were expressed and their localization was analyzed. The presence of cleavable mitochondrial targeting sequence was suggested by the N-terminal amino acid sequence analysis of AoPlaA-HA-His_6 purified on a Ni^<2+> column chromatography. In an independent experiment, a mutant strain ofA. olyzae disrupted for AoplaA was generated and tested for the phenotypic analyses.
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Research Products
(15 results)
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[Journal Article] Lysophosphatidylcholine protects cerebellar granule neurons from apoptotic cell death.2008
Author(s)
Ikeno, Y., Cheon, S. H., Konno, N., Nakamura, A., Kitamoto, K., Manabu Arioka
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Journal Title
J. Neurosci. Res. (印刷中)(掲載確定)
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Lysophosphatidylcholine protects cerebellar granule neurons from apoptotic cell death.2008
Author(s)
Ikeno, Y, Cheon, S.-h., Konno, N., Nakamura, A., Kitamoto, K., Arioka, M.
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Journal Title
J. Neurosci. Res., (in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Novel neurotrophic effects of sphingosylphosphorylcholine in cerebellar granule neurons and in PC12 cells.2007
Author(s)
Konno, N., Nakamura, A., Ikeno, Y., Cheon, S. H., Kitamoto, K., Arioka, M.
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Journal Title
Biochem. Biophys. Res. Commun. 364
Pages: 163-168
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Novel neurotrophic effects of sphingosylphosphorylcholine in cerebellar granule neurons and in PC12 cells.2007
Author(s)
Konno, N., Nakamura, A., Ikeno, Y., Cheon, S.-h., Kitamoto, K., Arioka, M.
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Journal Title
Biochem. Biophys. Res. Commun. 364(1)
Pages: 163-168
Description
「研究成果報告書概要(欧文)」より
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