2007 Fiscal Year Final Research Report Summary
Discovery of Food Microorganism : Fast and Effective Degradation of Intestinal Lipoperoxide and Hydrogen peroxide
| Project/Area Number |
18580083
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Tokyo University of Agriculture |
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Principal Investigator |
NIIMURA Youichi Tokyo University of Agriculture, Applied Bio-Science, Professor (00180563)
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| Project Period (FY) |
2006 – 2007
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| Keywords | lactic acid bacteria / dipoperoxide / degradation of lipoperoxide / degradation of hydrogen peroxide / Lactobacillus plantarum / probiotics / Pediococcus pentosaceus |
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| Research Abstract |
The objectives of this research are to discover probiotic lactic acid bacteria as a functional food product enabling strong lipoperoxide and hydrogen peroxide breakdown, and to accelerate its degrading capability being formed in the intestine.
In 2005, we developed an isolation medium separated off lactic acid bacteria that has lipoperoxide degradative enzyme. In addition, we isolated potential strains that possess strong degrading capability of hydrogen peroxide. In 2006 identifying useful strains from isolated lactic acid bacteria, we evaluated degrading capability of peroxide-decomposition mechanism.
After basic identification and grouping of lactic acid bacteria (lactic acid bacteria Manual, Asakura Bookshop), we screened a total ofl00 strains potentially applicable for functional food product and excluded potential pathogenic strains from isolated strains. 10 out 100 strains performing high lipoperoxide decomposition were full-identified through 16sDNA Sequence and Taxonomy and Phen
… More
otypic Analysis. As a result of the identification, they are classified as families of Lactobacillus plantarum and Pediococcus pentosaceus.
For measuring degradation activity of lipoperoxide and hydrogen peroxide, we prepared for Lactobacillus Standard GYP medium, and conditioned with aerobic culture at 37□ and then harvested. We utilized linoleic acid as a lipid peroxide and cumene peroxide as a model compound to measure its activity. In addition, hydrogen peroxide was used as a hydrophilic substrate. Among isolated strains, Lactobacillus plantarum Pan1-2 performed the highest degrading activity of lipoperoxide and Pediococcus pentosaceus Be-I performed the highest one of hydrogen peroxide. Pediococcus pentosaceus Be-I can scavenge both cumene peroxide and hydrogen peroxide.
In presence of butyl peroxide for identifying reaction product of L. plantarum Panl-2 and P pentosaceus Be-I, the strains of resting cell showed equal in reduction of butyl peroxide to butanol. As a result of that, it is suggested that the strains have 2-electron reduction of peroxide. Less
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Author(s)
Kouji, Takeda, Mayumi, Iizuka, Toshihiro, Watanabe, Junichi, Nakagawa, Shinji, Kawasaki, Youichi, Niimura
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Journal Title
The FEBS Journal vol. 274
Pages: 1318-1327
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] An O_2-inducible rubrerythrin-like protein, rubperoxin, is functional as a H_2O_2 reductase in an obligatory anaerobe Clostridium actoburylicurn2007
Author(s)
Shinji, Kawasaki, Masaki, Ono, YusukeWatamura, Yu, Sakai, Takumi, Satoh, Toshiaki Arai, Junichi Satoh, Youichi, Niimura
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Journal Title
FEBS Letters vol. 581
Pages: 2460-2464
Description
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[Journal Article] Response of the Microaerophilic Bifidobacterium species, B.boum and B. thermophilum, to oxygen2006
Author(s)
Shinji, Kawasaki, YusukeWatamura, Masaki, Ono, Toshihiro, Watanabe, Kouji, Takeda, Youichi, Niimura
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Journal Title
Appl. Environ. Microbial vol.72
Pages: 6854-6858
Description
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[Journal Article] Purification and Molecular Characterization of Subtilisin-Like Alkaline Protease BPP-A from Bacillus pumilus Strain MS-12006
Author(s)
Tatsuro, Miyaji, Yumi, Otta, Tomoyuki, Nakagawa, Toshihiro, Watanabe, Youichi, Niimura, Noboru, Tomizuka
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Journal Title
Letters in Applied Microbiology vol. 42
Pages: 242-247
Description
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[Journal Article] Purification and molecular chracterization of glutamyl endopeptidase from zein-utilizing Bacillus pumilus strain MS-12006
Author(s)
Tatsuro, Miyaji, Yumi, Otta, Tomoyuki, Nakagawa, Toshihiro, Watanabe, Youichi, Niimura, Noboru, Tomizuka
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Journal Title
Food preservation science vol. 32
Pages: 5-11
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 酸素と微生物の多様な生育2007
Author(s)
新村 洋一
Organizer
日本農化学大会ランチョンセミナー基調講演
Place of Presentation
東京農業大学
Year and Date
2007-03-26
Description
「研究成果報告書概要(和文)」より
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