2007 Fiscal Year Final Research Report Summary
Molecular mechanisms in regulation of gene expression in Protobothrops flavoviridis venom gland.
| Project/Area Number |
18580098
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Sojo University |
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Principal Investigator |
ODA Naoko (小田 直子) Sojo University, Faculty of Pharmaceutical Sciences, Professor (70211828)
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| Co-Investigator(Kenkyū-buntansha) |
KARIU Tohru Sojo University, Faculty of Pharmaceutical Sciences, Lecturer (10412735)
TAKAZAKI Shinya Sojo University, Faculty of Pharmaceutical Sciences, Research assistant (90435149)
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| Project Period (FY) |
2006 – 2007
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| Keywords | venom / transcription / ハブ(ヘビ) |
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| Research Abstract |
Protobothrops flavoviridis snakes inhabit the southwestern islands of Japan: Amami-Oshima, Tokunoshima and Okinawa. P flavoviridis venom contains abundant biologically active proteins. In order to understand molecular mechanisms of transcription and translation in P. flavoviridis venom gland, the following two subjects have been studied. 1) Transcriptomics of Amami-Oshima P. flavoviridis venom gland, and proteomics of P flavoviridis venoms from Amami-Oshima, Tokunoshima and Okinawa. The detailed study on transcriptomics of P. flavoviridis venom gland led to the finding of several novel genes participating in transcription and translation in addition to toxic protein transcripts. The comparative proteomics study on P. flavoviridis venoms from the three islands by means of two dimensional gel electrophoresis and ESI-Q-TOF mass spectrometry disclosed that island specific expression or mutation have occurred in toxic proteins in individual islands. 2) An attempt to identify venom gland specific transcription factors in P flavoviridis. Five promotor sequences of about 40 by lengths of the genes encoding venom proteins were chosen and tested by means of gel-shift assay whether they are able to interact with protein (s) in P flavoviridis venom gland extracts. One candidate was verified to bind to a protein from the extracts. Identification of this protein is under way using ESI-Q-TOF analysis.
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Research Products
(42 results)
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[Presentation] ハブ毒中の新規な補体活性化因子の同定と諸性質2008
Author(s)
村上 達夫, 鈴木 美緒, 今村 隆寿, 服部 正策, 千々岩 崇仁, 大野 素徳, 上田 直子
Organizer
日本薬学会 第128年会
Place of Presentation
横浜パシフィコ横浜
Year and Date
2008-03-28
Description
「研究成果報告書概要(和文)」より
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[Presentation] ハブ毒中のC3/C5コンベルターゼの精製と諸性質2007
Author(s)
鈴木 美緒, 今村 隆久, 村上 達夫, 服部 正策, 千々岩 崇仁, 大野 素徳, 上田 直子
Organizer
第30回日本分子生物学会年会・第80回日本生化学会年会 合同年会
Place of Presentation
横浜パシフィコ横浜
Year and Date
2007-12-13
Description
「研究成果報告書概要(和文)」より
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[Presentation] Evolution of Protobothrops elegans venom phospholipase A_22006
Author(s)
Masayo, Yatsui, Takashi Nagata, Emi Tokunaga, Yuki Kudo, Ryo Ikeda, Takahito Chijiwa, Naoko Oda-Ueda, Shosaku Hattori, Motonori Ohno
Organizer
20th IUBMB International Biochemistry and Molecular Biology and 11th FAOBMB Congress
Place of Presentation
Kyoto Japan
Year and Date
2006-06-19
Description
「研究成果報告書概要(和文)」より
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[Presentation] Evolution of Protobothrops elegans venom phospholipase A^22006
Author(s)
Masayo, Yatsui, Takashi Nagata, Emi Tokunaga, Yuki Kudo, RyoIkeda, Takahito Chijiwa, Naoko Oda-Ueda, Shosaku Hattori, Motonori Ohno.
Organizer
20th IUBMB International Biochemistry and Molecular Biology and 11th FAOBMB Congress.
Place of Presentation
Kyoto Japan
Year and Date
2006-06-19
Description
「研究成果報告書概要(欧文)」より
