2007 Fiscal Year Final Research Report Summary
Screening of renin inhibitory activity in Foods and its functional analysis.
| Project/Area Number |
18580134
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Akita Prefectural Agriculture, Forestry and Fisheries Research Center |
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Principal Investigator |
TAKAHASHI Saori Akita Prefectural Agriculture, Forestry and Fisheries Research Center, Akita Research Institute for Food and Brewing, Chief Researcher (10142184)
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| Co-Investigator(Kenkyū-buntansha) |
HORI Kazuyuki Akita Prefectural Agriculture, Forestry, and Fisheries Research Center, Akita Research Institute for Food and Brewing, Senior Researcher (50181516)
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| Project Period (FY) |
2006 – 2007
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| Keywords | renin / renin-angiotensin system / synthetic substrate / inhibitor / insect cells / expression / soybean / legumes |
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| Research Abstract |
The renin-angiotensin-aldosterone system (RAS) plays an essential role in blood pressure control in animals. Renin catalyzes the liberation of angiotensin I from plasma substrate angiotensinogen. The produced decapeptide angiotensin I is an inactive peptide and activated by angiotensin converting enzyme (ACE). ACE cleaves C-terminal dipeptide from angiotensin I. The produced angiotensin II raises blood pressure by vasoconstriction as well as stimulation of the synthesis and release of aldosterone. Thus, renin is a key enzyme of RAS. ACE has been used as a target enzyme in RAS for screening inhibitors because of its simple assay method; however, renin is a rate-limiting enzyme in RAS, so it was not used because the measurement is very complicated. In the present study, we expressed recombinant human renin in Escherichia coil cells, refolded and activated by trypsin. Using recombinant human renin as a target enzyme, we screened the renin inhibitory activity in fermented soybean paste (miso) and found that soybean, the major ingredient of miso, had renin inhibitory activity. Moreover, we expressed recombinant human renin in insect Sf-9 cells and found that mature active renin accumulated in the late stage of cultivation. Using the medium, we simply purified recombinant human renin and characterized various properties of it. Our method should provide a large quantity of recombinant human renin using the baculovirus expression system.
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[Presentation] Renin inhibitory activity in beans.(Japanese)2008
Author(s)
Saori Takahashi, Kazuyuki Hori, Masanori Kumagai, Saburou Wakabayashi
Organizer
2008 Annual Meeting of Japan Societry for Bioscience, Biotechnology, and Agrochemistry
Place of Presentation
Meijo University(Nagogy)
Year and Date
2008-03-28
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Renin inhibitor in legumes.2007
Author(s)
Saori Takahashi, Kazuyuki Hori, Masanori Kumagai, Saburou Wakabayashi
Organizer
ICOFF2007, International Conference on Food Factors for Health Promotion
Place of Presentation
Kyoto International Conference Hall(Kyoto)
Year and Date
2007-11-30
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Renin inhibitor from foods.(Japanese)2007
Author(s)
Saori Takahashi, Takayuki Watanabe, Hironobu Ogasawara, Masanori Kumagai, Keishi Hata, Kazuyuki Hori, Mari Sugawara
Organizer
54th Annual Meeting of Japanese Society for Food Science and Technlogy
Place of Presentation
Nakamura-Gakuen University(Fukuoka)
Year and Date
2007-09-08
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Renin inhibitor form miso and soybean.(Japanese)2007
Author(s)
Saori Takahashi, Hironobu Ogasawara, Takayuki Watanabe, Masanori Kumagai, Kaoru Owari, Kazuyuki Hori
Organizer
2007 Annual Meeting of Japan Society for Bioscience, Biotechnology and Agrochemistry
Place of Presentation
Tokyo University of Agriculture(Tokyo)
Year and Date
2007-03-25
Description
「研究成果報告書概要(欧文)」より
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