2007 Fiscal Year Final Research Report Summary
Understanding the mechanism underlying the adaption of betanodaviruses to host termperatures.
| Project/Area Number |
18580185
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Hiroshima University |
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Principal Investigator |
OKINAKA Yasushi Hiroshima University, Graduate School of Biosphere Science, Associate Professor (80363034)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAI Toshihiro Hiroshima University, Graduate School of Biosphere Science, Professor (60164117)
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| Project Period (FY) |
2006 – 2007
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| Keywords | betanodavirns / temperature sensitivity / reassortant virus / chimeric virus / SJNNV / RGNNV |
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| Research Abstract |
Betanodaviruses, the causal agents of viral nervous necrosis in marine fish, have bipartite positive-sense RNA genomes. The larger genomic segment RNA1 (3.1 kb) encodes an RNA-dependent RNA polymerase (protein A) and the smaller genomic segment RNA2 (1A kb) Codes for the coat protein The viruses can be classified into four genotypes, designated SJNNV, RGNNV, TPNNV and BFNNV, based on similarities in the partial RNA2 sequence. The optimal temperatures for multiplication of these viruses were 20-25℃ (SJNNV), 25-30℃ (RGNNV), 20℃ (TPNNV) and 15-20℃ (BFNNV). However, little is known about the mechanisms underlying temperature sensitivities of these viruses. In order to know the segment(s) that control temperature sensitivity, we first constructed two reassortants between SJNNV and RGNNV. Multiplication of these reassortantsandSINNVinculturedfishcellsat30℃ was strongly inhibited compared with those of RONNY. The viral multiplication levels were well correlated with those of viral RNA replication. These results indicate that both. RNA1 and RNA2 control temperature sensitivities of betanodaviruses , via modulating RNA replication or earlier virus multiplication processes. Second, to identify an RNA1 region(s) that confers temperature sensitivities, we constructed 10 mutated viruses having chimeric RNA1 segments between SJNNV and RGNNV. Evaluation of temperature sensitivities in these chimeric viruses showed that the 5' terminus of the protein A-coding region in RNA1 strongly control temperature sensitivities The ,N-terminal portion of protein A is known to serve a mitochondrial targeting signal rather than function as an enzymatic domain.
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[Journal Article] The variable region of RNA2 is sufficient to determine host specificity in betanodaviruses2008
Author(s)
Ito, Y., Oldnaka, Y., Mori, K., Sugaya, T., Nislaioka, T., Oka, M., Nalcai, T
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Journal Title
Dis. Aquat. Org (in press)(peer reviewed)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Phylogenetic analysis of betanodavirus RNA2 identified from wild marine fish in oceanic regions2008
Author(s)
Sakamoto, T., Okinaka, Y., Mori,K., Sugaya, T., Nishioka, T., Yamashita, H., Oka, M., Nakai, T
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Journal Title
Fish Pathol 43(peer reviewed)
Pages: 19-27
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Characterization of Striped jack nervous necrosis virus subgenomic RNA3 and biological activities of its encoded protein B22007
Author(s)
Okinaka, Y., Iwamoto, T., Mise, K., Takeda, A., Mori, K., Arimoto, M., Okuno, T., Nakai, T
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Journal Title
Sufficiency Economy Philosophy for the Sustained Development of Fishery and Agriculture (Kasetsart University, Thailand) (not peer reviewed)
Pages: 303-319
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Genotype-specific binding of betanodaviruses to sevenband grouper cultured cells, SOF2008
Author(s)
Kawato, Y., Okinaka, Y., Yamashita, H., Nakai, T
Organizer
7th Symposium on Diseases in Asian Aquaculture, Taipei, Taiwan
Year and Date
20080622-26
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Characterization of Striped jacknervous necrosis virus subgenomic RNA3 and biological activities of its encoded protein B22007
Author(s)
Okinaka, Y., Iwamoto, T., Mise, K., Takeda, A., Mori, K., Arimoto, M., Okuno, T., Nakai, T
Organizer
JSPS-NRCT Joint Seminar, Bangkok, Thailand
Year and Date
20071217-18
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Phylogenetic analysis of the coat protein genes of betanodaviruses from wild marine fish in Japan2006
Author(s)
Sakamoto, T., Okinaka, Y., Mori, K., Sugaya, T., Nishioka, T., Oka, M., Yamashita, H., Nakai, T
Organizer
The First International Symposium on Viral Nervous Necrosis of Fish, Hiroshima, Japan
Year and Date
20061128-1201
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Detection of betanodaviruses in wild marine fishes from the coasts of Japan and East China Sea2006
Author(s)
Mori, K., Sugaya, T., Nishioka, T., Oka, M., Tukamoto, Y., Gomez, D. K., Okinaka, Y., Nakai, T
Organizer
The First International Symposium on Viral Nervous Necrosis of Fish, Hiroshima, Japan
Year and Date
20061128-1201
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Genetic variablity of betanodaviruses and its application to epidemiological" study2006
Author(s)
Sugaya, T., Mori, K., Nishioka, T., Mushiake, K., Okinake, Y., Nakai, T
Organizer
The First International Symposium on Viral Nervous Necrosis of Fish, Hiroshima, Japan
Year and Date
20061128-1201
Description
「研究成果報告書概要(欧文)」より
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[Presentation] A trial to establish new fish cell lines susceptible to piscine nodavirus2006
Author(s)
Kuroda, A., Tsutsumi, N., Mori, K., Yamashita, H., Tanaka, S., Hanyu, K., Okinaka, Y., Nagai, S., Nakai, T
Organizer
The First International Symposium on Viral Nervous Necrosis of Fish, Hiroshima, Japan
Year and Date
20061128-1201
Description
「研究成果報告書概要(欧文)」より
