2007 Fiscal Year Final Research Report Summary
Development of techniques for in vitro production of good quality eggs by elucidation of mechanisms of oocyte cytoplasmic maturation in teleost
| Project/Area Number |
18580187
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | University of Miyazaki |
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Principal Investigator |
KAGAWA Hirohiko University of Miyazaki, Faculty of Agriculture, Professor (60169381)
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| Project Period (FY) |
2006 – 2007
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| Keywords | eel / oocyte / oocyte maturation / cytoplasmic maturation / aquaporin / in vitro / egg quality / maturation-inducing steroid |
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| Research Abstract |
To elucidate mechanisms of oocyte cytoplasmic maturation (such as a hydration, liquid coalescence and clearing of the ooplasm) during oocyte maturation and to develop techniques for in vitro production of good quality eggs, the present study examined the following studies in the Japanese eel spawn buoyant eggs.
1. The present study shows the process of oocyte cytoplasmic maturation during de novo oocyte maturation in the Japanese eel. Moreover, we also developed the incubation techniques for mimic the cytoplasmic maturation occurred in vivo. Using the techniques we developed, we found that cytoplasmic maturation was induced by the maturation-inducing steroid that was synthesized in the ovarian follicles by the stimulation of gonadotropin. Cytoplasmic maturation induced by maturation-inducing steroid may be mediated by protein synthesis in the oocyte. IGF-I, but not GAP junction may be involved in the cytoplasmic maturation of eel oocytes.
2. The present study also indicates that oocyte h
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ydration is mediated by yolk proteolysis which creates the osmotic driving force and aquaporin possibly facilitates water uptake into oocytes. Moreover, the study indicates that the ovarian follicles in the Japanese eel may play an essential role in oocyte hydration, although the precise role must be identified in the further research.
3. The present study also examined the Japanese eel for the presence and cellular distribution of eel AQP-1o in the ovarian follicles during oocyte growth, oocyte maturation and ovulation by the immunocytochemistry. Anti-AQP-1o antibody was raised in a rabbit against a synthetic peptide corresponding to a part of the C-terminal region of eel AQP-1o. Immunoreactivity was first observed in the cytoplasm around the yolk globules which were located in the peripheral region of the oocytes during the primary yolk globule stage; thereafter, the immunoreactivity was observed throughout the cytoplasm of the oocyte as vitellogenesis progressed. The immunoreactivity became localized around the large membrane-limited yolk masses which were formed by the fusion of yolk globules. It also localized below the oocyte plasma membrane in the migratory nucleus stage oocytes, mature oocytes and ovulated eggs. These results are different from those observed in the oocytes of gilthead sea bream and suggest the existence of a distinct and characteristic mechanism of oocyte hydration in the eel.
4. We tried to develop the techniques for in vitro production of good quality eggs in the Japanese eel. Although we found the oocyte maturation and ovulation occurred at 12 and 18 hours after incubation with maturation-inducing steroid respectively, we could not obtained fertilized eggs. Further studies are necessary to develop the techniques for obtaining good quality eggs in vitro. Less
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Research Products
(22 results)
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[Journal Article] Egg yolk proteins in gray mullet(Mugil cephalus) : Purification and classification of multiple lipovitellins and other vitellogenin-derived yolk proteins and molecular cloning of the parent vitellogenin genes.2007
Author(s)
Amano, H., Fujita, T., Hiramatsu, N., Shimizu, M., Sawaguchi, S., Matsubara, T., Kagawa, H., Nagae, M., Sullivan, C.V., Hara, A.
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Journal Title
Journal Experimental Zoology Part A 307
Pages: 324-341
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Expression of growth hormone family and growth hormone receptor during early development in the Japanese eel(Anguilla japonica)2006
Author(s)
Ozaki, Y., Fukada, H., Tanaka, H., Kagawa, H., Ohta, H., Adachi, S., Hara, A., Yamauchi, K.
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Journal Title
Comparative Biochemistry and Physiology Part B 145
Pages: 27-34
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Molecular characterization of three forms of vitellogenin and their yolk protein products during oocyte growth and maturation in red seabream(Pagrus major), a marine teleost spawning pelagic eggs.2006
Author(s)
Sawaguchi, S., Kagawa, H., Ohkubo, N., Hiramatsu, N., Sullivan, C.V., Matsubara, T.
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Journal Title
Molecular Reproduction and Development 73
Pages: 719-736
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Effects of administration of various hormones Using an osmotic pump on sexual maturation in the female Japanese eel.2008
Author(s)
Fujie, N., Adachi, J., Nishi, A., Hashimoto, H., Kaji, S., Kishi, T., Kagawa, H.
Organizer
The Annual Meeting of the Japanese Society of Fisheries Science
Year and Date
20080000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Effects of HgCl_2 and bafilomycinA1 on oocyte hydration during oocyte maturation in the Japanese eel.2007
Author(s)
Horiuchi, Y., Adachi, J., Nishi, A., Hashimoto, H., Kaji, S., Kasuga, Y., Kagawa, H.
Organizer
The Annual Meeting of the Japanese Society of Fisheries Science
Year and Date
20070000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Effects of administration of salmon pituitary extracts using an osmotic pump on sexual maturation in the female Japanese eel.2007
Author(s)
Morimoto, R., Adachi, J., Nishi, A., Hashimoto, H., Kaji, S., Horiuchi, Y., Kagawa, H.
Organizer
The Annual Meeting of the Japanese Society of Fisheries Science
Year and Date
20070000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Aquaporin and proteolysis of yolk and ovarian follicles play essential roles in the regulation of oocyte hydration during oocyte maturation in the Japanese eel.2007
Author(s)
Horiuchi, Y., Adachi, J., Nishi, A., Hashimoto, H., Kaji, S., Kasuga, Y., Kagawa, H.
Organizer
8th International Symposium of Physiology of Fish
Year and Date
20070000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Cytoplasmic maturation during oocyte maturation and ovulation in the Japanese eel.2006
Author(s)
Horiuchi, Y., Adachi, J., Nishi, A., Hashimoto, H., Kaji, S., Kasuga, Y., Kagawa, H.
Organizer
The Annual Meeting of the Japanese Society of Fisheries Science
Year and Date
20060000
Description
「研究成果報告書概要(欧文)」より
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[Book] 水産大百科事典2006
Author(s)
川口 恭一, 他
Total Pages
808
Publisher
朝倉書店
Description
「研究成果報告書概要(和文)」より
