2007 Fiscal Year Final Research Report Summary
INVESTIGATION OF FISH ROE ALLERGEN AND ITS CROSS-REACTIVITY
| Project/Area Number |
18580200
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
SAEKI Hiroki Hokkaido University, FACULTY OF FISHERIES SCIENCES, Professor (90250505)
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| Co-Investigator(Kenkyū-buntansha) |
HARA Akihiko HOKKAIDO UNIVERSITY, FACULTY OF FISHERIES SCIENCES, PROFESSOR (40091483)
SHIMIZU Yutaka HOKKAIDO UNIVERSITY, FACULTY OF FISHERIES SCIENCES, TECHNICALSTUFF (00374629)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Food allergy / Fish roe / allergen / vitellogenin / β'-conmponent / IKURA / TARAKO / Yolk protein |
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| Research Abstract |
Fish roe such as salmon, walleye pollack, and herring roes are popularly consumed in Japan and much cases of fish-roe-allergy, particularly in childhood, have been reported last decade. However; there is little information on fish roe allergens.
The objective of this study is to identify fish roe allergens and to investigate the IgE cross-reactivity among various kinds of fish roes.
The results were as follows: (1) Eleven kinds of fish roes (Churn salmon: Oncorhynchus keta, Sakhalin taimen: Hucho perryi, rainbow trout: Oncorlhynchus mykiss Japanese char: Salvelinus leucomaenis, dolly vuuden: Salvelinus malma, walleye pollack: Theragra chalcogramma, dusky sole Lepidopsetta mochigare, Slime flounder:Microstomus achne, atka mackerel: Plecurogrammus azonus, sishamo smelt: Spirinchus lanceolatus capelin: Mallotus villosus) contained yolk protein that reacted with anti chuun-salmon-β'-component rabbit IgG (anti-β) and they also reacted with specific IgE in chum salmon roe-allergic patients' se
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(2) The major allergens in salmonid fish roes were β'-component and its related fragments.
(3) There were IgE cross-reactivity among salmonid roe β'-components, and the IgE cross--reactivity was also confirmed between churn salmon and walleye pollack roes.
(4) Amino acid sequence of churn salmon β'-component (Mw. 16,000) was examined and the sequences of 91 residues (about 60% of the total sequence) were determined by peptide mapping using the complete sequence of rainbow trout viltellogenin.
(5) cDNA encoding β'-component was obtained using mRNAs extracted firm chum salmon liver and the encoded amino acid sequence was coincided with the sequence obtained by the direct peptide mapping.
(6) β'-component like protein (Mw 17,000) was purified firm walleye pollack roe, and about 70% of the total amino acid sequence was determined using an inside arrangement of Atlantic cod vitellogenin as a template.
These findings would contribute understanding fish roe allergy and improvement of QOL of allergic patients. Less
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Research Products
(12 results)
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[Presentation] Epitopes in IKURA and TARAKO allergen2008
Author(s)
S. Koike, Y Shimizu, K. Watanabe, H. Kishimura, A. Hara, H. Saeki.
Organizer
Annual meeting of The Japanese Society of Fisheries Science
Place of Presentation
University, Shimizu campus
Year and Date
2008-03-31
Description
「研究成果報告書概要(欧文)」より
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