2007 Fiscal Year Final Research Report Summary
Production of useful substances by factory of elite plant cells
| Project/Area Number |
18580255
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Agricultural environmental engineering
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| Research Institution | Nagasaki University |
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Principal Investigator |
KITAMURA Yoshie Nagasaki University, Faculty of Environmental Studies, Professor (40108337)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMOMATI Takashi Nagasaki University, Faculty of Environmental Studies, Associate Professor (60249886)
TOMINAGA Yoshinori Nagasaki University, Faculty of Environmental Studies, Professor (70100881)
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| Project Period (FY) |
2006 – 2007
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| Keywords | elite plant cell / useful substance production / metabolic engineering / tissue culture / gene insertion |
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| Research Abstract |
Plants can produce complex organic compounds from simple inorganic compounds in their own micro cell factory. Using this sophisticated function, productions of useful industrial materials, which are used to be synthesized by chemical engineering, were performed with in vitro plant tissue culture system. To establish elite clones expressing high productivity of aimed compounds, metabolic engineering by gene introduction, induction of suppressed metabolic pathway with stress treatments, and/or selection of superior clones were carried out as follows:
1.p-Hydroxy benzoic acid(pHBA)production by Beta vulgaris hairy roots
The gene of bacterial HCHL, which converts ferulic acid(C_6-C_3) to vanillin(C_6-C_1), was introduced to Beta vulgaris using Agrobacterium rhizogenes. A selected elite hairy root clone produced glucose ester of pHBA(C_1)up to 14% on the basis of dry weight
2. Scopolamine production by Duboisia leichhardtii hairy roots
Plant H6H, which converts hyoscyamine to scopolamine, was introduced into Duboisia leichhardtii, same as above, establishing an elite clone that produced more than 95% of scopolamine in total tropane alkaloids.
3.Coumarin production by Glehnia littoralis organ cultures
1)Yeast extract induced furanocoumarin in leaf cultures, but not in root cultures.
2)Ascorbic acid highly induced furanocoumarin in root culture as well as leaf culture. This is the first case of furanocoumarin induction in a root culture system.
4.Riboflavin(vitamin B_2) production by Hyoscyamus albus hairy roots
Riboflavin was excreted by Hyoscyamus albus hairy roots into the medium cultured under iron-deficient condition. The productivity was dependent to size and number of root-tip. Most producing elite clone was chosen for further study.
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[Journal Article] Synthesis and fluorescence of 2H-pyrone derivatives for organic light-emitting diodes(OLED)2007
Author(s)
Mizuyama, N., Murakami, Y., Kohra, S., Ueda, K., Hiraoka, K., Nagaoka, J., Takahashi, K., Shigemitsu, Y., Tominaga, Y
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Journal Title
Journal of Heterocyclic Chemistry 44
Pages: 115-132
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Exogenous plant H6H but not bacterial HCHL gene is expressed in Duboisia leichhardtii hairy roots and affects tropane alkaloid production2006
Author(s)
Rahman, L., Kitamura, Y., Yamaguchi, J., Mukai, M., Akiyama, K., Yamamoto, H., Muranaka, T., Ikenaga, T
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Journal Title
Enzyme and Microbial Technology 39
Pages: 1183-1189
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Synthesis and steady-state spectroscopic study of 5-aryl-2, 2'-bipyridyls. New fluorescent compounds in solid state2006
Author(s)
Mizuyama, N., Tominaga, Y., Kohra, S., Ueda, K., Hirayama, S., Shigemitsu, Y
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Journal Title
Bulletin of the Chemical Society of Japan 79
Pages: 602-611
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Difference of furanocoumarin production between two kinds of cells of Glehnia littoralis and constitutive expression of BMT2007
Author(s)
Ishikawa, A., Kobayashi, N., Itoh, Y., Yamada, A., Ozeki, Y., Kitamura, Y
Organizer
The 25th conference of Japanese society for Plant Cell and Molecular Biology
Place of Presentation
Tiba
Year and Date
20070808-09
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 変異ニンジン懸濁培養細胞株におけるアントシアニンアシル基転移酸素の特性2007
Author(s)
奥田 裕樹, 阿部 裕, 野田 尚信, 鈴木 正彦, 寺坂 和祥, 水上 元, 石川 彩, 小林 信之, 北村 美江, 山田 晃世, 小関 良宏
Organizer
第48回日本植物生理学会年会
Place of Presentation
松山
Year and Date
20070328-30
Description
「研究成果報告書概要(和文)」より
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[Presentation] Characterization of anthocyanin acyltransferase in mutant carrot cell suspension culturesq2007
Author(s)
Okuda, H., Abe, H., Noda, H., Suzuki, M., Terasaka, K., Mizukami, H., Ishikawa, A., Kobayashi, N., Kitamura, Y., Yamada, A., Ozeki, Y
Organizer
The 48th Annual meeting of Japanese society for Plant physiology
Year and Date
20070328-30
Description
「研究成果報告書概要(欧文)」より
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[Presentation] HCHL Bacterial gene expression in hairy roots of sugar beet causes the production of Free 4-hydroxybenzoic acid2006
Author(s)
Kitamura, Y., Rahman, LU., Maeda, I., Parr, A., Narbard, A., Walton, N., Ikenaga, T
Organizer
11th IAPTC&B Congress
Place of Presentation
Beijin
Year and Date
20060813-18
Description
「研究成果報告書概要(欧文)」より
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