2007 Fiscal Year Final Research Report Summary
Development of in vitro bioassay for avian leptin
| Project/Area Number |
18580290
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Kagawa University |
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Principal Investigator |
OHKUBO Takeshi Kagawa University, Faculty of Agriculture, Associate Professor (70233070)
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| Project Period (FY) |
2006 – 2007
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| Keywords | chicken / leptin / Bioassay / signal transduction |
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| Research Abstract |
We established a cell line stably expressing chicken leptin receptor (chLEPR) to develop a bioassay measuring avian leptin. The established cell line termed CHO-chLEPR expresses 180 kDa protein which specifically cross-reacted with anti-chLEPR monoclonal antibody. Murine leptin specifically bound with the chLEPR, leading to phosphorylation of signal transducers and activators of transcription 3 (STAT3) and resulted activation of luciferase in the cells. In addition, Janus kinase 2 (JAK2) inhibitor partially blocked leptin-induced luciferase activation and RNA interference for chLEPR reduced the induction rate of luciferase activity by leptin in CHO-chLEPR cells. These results strongly suggest that chLEPR is functional in activating the JAK-STAT pathway, which may indicate that the LEPR expressed in chicken tissues is capable of binding endogenous ligand as well as exogenous mammalian leptin, leading to physiological actions. However, we did not find any induction of luciferase activity by chicken serum or tissue extracts. This might have been because the level of leptin in the chicken is too low for the signal to have been detected in the present assay. Therefore further experiments, such as isolating leptin inducible gene from chicken, are required to improve sensitivity of low-dose of leptin in the in vitro bioassay for avian leptin.
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Research Products
(28 results)
