2007 Fiscal Year Final Research Report Summary
Comparative genomics of staphylococcal enterotoxin gene family
| Project/Area Number |
18580304
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied veterinary science
|
|---|
| Research Institution | Iwate University |
|---|
Principal Investigator |
OMOE Katsuhiko Iwate University, Faculty of Agriculture, Associate Professor (60224309)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SHINAGAWA Kunihiro Faculty of Agriculture, 農学部, Professor (60133906)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Keywords | S. aureus / Staphylococcal enterotoxin / recombinant DNA / genome biology / diagnosis / pathogenicity islands |
|---|
| Research Abstract |
In recent years, many new staphylococcal enterotoxins (SEs) have been reported. It has been known that certain SE genes are associated with mobile genetic elements such as pathogenicity islands, prophages, and plasmids. These facts imply that superantigenic toxin genes are transferred horizontally between staphylococcal strains. There is a possibility that these mobile genetic elements have played an important role in the evolution of S. aureus as a pathogen. To investigate pathobiology and epidemiology of these newly identified Ses, we had undertaken studies shown in below.
1. To detect and characterize unknown SE gene encoding pathogenicity islands, we have developed a new method, named pathogenicity islands scanning. Based on full genome sequences of several S. aureus, we designed PCR primers to amplify entire region of pathogenicity islands. Amplicons were subjected to restriction fragments length polymorphism (RFLP) analysis. Using pathogenicity islands scanning/RFLP, We have found
… More
that SEB-related pathogenicity islands are classified into at least 5 groups. Also, we have determined complete nucleotide sequence of selj/selr encoding plasmid pF5 using shotgun sequencing strategy. This plasmid is encoding two novel enterotoxin gene, ses and set, in addition to selj and selr. In addition, we developed several diagnostic methods including immuno-PCR sensitive detection of SEs, cultivation/PCR of sensitive detection of S. aureus, and multiplex PCR for comprehensive detection of SE/SE1 genes.
2. We characterized two novel staphylococcal entertoxins SES and SET. Recombinant (r)SES specifically stimulated human T cells in T cell receptor (TCR) Vβ9-specific manner in the presence of MHC class II^+ antigen presenting cells (APCs). rSET also stimulated T cells in the presence of MHC class II^+ APC, although its Vβ skewing was not found in reactive T cells. Subsequently, we examined the emetic activity of SES and SET. We also studied SEIR to determine emetic activity in primates. This toxin was identified in previous studies but not examined in terms of possessing emetic activity for primates. rSES induced emetic reactions in 2 of 4 monkeys at a dose of 100 μg/kg within 5 hours of intragastric administration. In one monkey, rSET induced a delayed reaction (24 hours post-administration) at a dose of 100 μg/kg and in the other one, reaction occurred 5 days post-administration. Also, we investigated a mechanism of SEA-induced emesis using a small emetic animal model, House musk shrew (Suncus murinus). SEA induces serotonin (5-hydroxytryptamine, 5-HT) release in intestine, and that the 5-HT3 receptor on vagal afferent neurons are essential for SEA induced emesis. Less
|
Research Products
(12 results)
-
-
-
-
-
[Journal Article] A quantitative real-time immuno-PCR approach for detection of staphylococcal enterotoxins2007
Author(s)
Fischer, A., von Eiff, C., Kuczius, T., Omoe, K., Peters, G. and Becker, K.
-
Journal Title
J. Mol. Med. 85
Pages: 461-469
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Staphylococcal enterotoxin induces emesis through increasing serotonin release in intestine and it is down-regulated by cannabinoid receptor 12007
Author(s)
Hu, D. -L., Zhu, G., Mori, F., Omoe, K., Okada, M., Wakabayashi, K., Kaneko, S., Shinagawa, K. and Nakane, A.
-
Journal Title
Cell. Microbiol 9
Pages: 2267-2277
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
[Journal Article] Food poisoning attributable to Staphylococus aureus deficient in all of the staphylococcal enterotoxin gene so far reported.2006
Author(s)
Kuramoto, S., Kodama, H., Yamada, K., Inui, I., Kitagawa, E., Kawakami, K., Satomi, R., Ikawa, A., Omoe, K., and Shinagawa, K.
-
Journal Title
Jpn. J. Infect. Dis. 59
Pages: 347
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Intranasal vaccination with a double mutant of staphylococcal enterotoxin C provides protection against Staphylococcus aureus infection2006
Author(s)
Hu, D. -L., Omoe, K., Narita, K., Cui, J. -C., Shinagawa, K. and Nakane, A.
-
Journal Title
Mirobes Infect 8
Pages: 2841-2848
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Comparative prevalence of superantigenic toxin genes in methicillin-resistant and methicillin-susceptible Staphylococcus aureusisolates
Author(s)
Hu, D.-L., Omoe, K., Inoue, F., Kasai, T., Yasujima, M., Shinagawa, K. and Nakane, A.
-
Journal Title
J. Med. Microbiol. (In press)
Description
「研究成果報告書概要(欧文)」より
