Elucidation of the mechanism of functional expression of metal-porphyrin immobilized carrier as an artificial solid catalyst.

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 18590033
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Okayama University
• Principal Investigator: SAITO Yutaka Okayama University, Graduate School of Medicine and Dentistry and Pharmaceutical Sciences, Professor (20025711)
• Project Period (FY): 2006 – 2007
• Keywords: Metal-porphyrin / Immobilized carrier / Artificial solid catalyst / Adrenaline / Peroxidase / Vibrational spectrum / Resonance Raman spectrum / Mechanism

Research Abstract

The aim of this study is to teach measurement skill to young researchers through development of artificial catalyst and elucidation of the mechanism of catalytic activity. Although applicant has already developed several artificial catalysts, the mechanism of the catalysts has not been completely elucidated. Then, development of new artificial catalysts and elucidation of the mechanism were attempted. The results obtained in this research are listed below.
1. Carriers modified with Mn-porphyrin as an artificial catalyst for oxidation of catecholamines such as adrenaline were developed. The carriers can oxidize adrenaline to adrenochrome and adrenolutin. It was suggested that this oxidative catalytic function is attributable to the redox cycle of Mn^<3+> 〓 Mn^<4+>
2. To develop a new application of metal-porphyrin immobilized carrier, its applicability to the stationary phase for HPLC was investigated.
3. The peroxidase-like activity of metal-octabromo-porphyrin such as Fe^<3+>-OBTM, Co^<3+>-OBTM and Mn^<3+>-OBTM on organic peroxide was examined. It was revealed that Fe^<3+>-OBTM has an activity equivalent to conventional enzyme. To elucidate the mechanism of peroxidase-like activity of Fe^<3+>-OBTM by resonance Raman spectra, the flow cell system was developed. The results obtained in this study revealed that said activity is attributable to the repeated redox cycle of the central metal of Fe^<3+>-OBTM, i.e., Fe^<3+>〓Fe^<4+>.
The measurement skill for the vibrational spectra such as the resonance Raman specrreum could be imparted to young researchers through the research from 2006 to 2007.

Research Products

• [Journal Article] Ion-Exchange Resins Modified with Metal-Porphyrin as a Catalysis for Oxidation of Epinephrine (Adrenaline) (2008)
  • Author(s): Kitamura Y., Mifune M., Takatsuki T., Iwasaki T., Kawamoto M., Iwado A., Chikuma M. and Saito Y.
  • Journal Title: Catalysis Communications 9(2) Pages: 224-228
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Ion-Exchange Resins Modified with Metal-Porphyrin as a Catalysis for Oxidation of Epinephrine (Adrenaline) (2008)
  • Author(s): Kitamura, Y., Mifune, M., Takatsuki, T., Iwasaki, T., Kawamoto, M., Iwado, A., Chikuma, M., Saito, Y
  • Journal Title: Catalysis Communications 9(2) Pages: 224-228
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Catalytic Activity of Silica Gels Bound Manganese (III)-Porphyrin on Oxidative Reaction of Adrenaline (2007)
  • Author(s): Kitamura Y., Takatsuki T., Kawamoto M., Saito M., Iwado A., Tsukamoto I., Mifune M., Saito Y.
  • Journal Title: Chem. Pharm. Bull. 55(3) Pages: 500-502
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Peroxidase-like catalytic activity of Mn^<3+>-octabromo-tetrakis(4-sulfophenyl)-porphine on linoleate hydroperoxide and its analytical application (2007)
  • Author(s): Mifune M., Kamiguchi H, Tai T., Kuremoto S., Yamamoto M., Tsukamoto I., Saito M., Kitamura Y., Saito Y.
  • Journal Title: Talanta 71(1) Pages: 456-461
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Catalytic Activity of Silica Gels Bound Manganese(III)- Porphyrin on Oxidative Reaction of Adrenaline (2007)
  • Author(s): Kitamura, Y., Takatsuki, T., Kawamoto, M., Saito, M., lwado, A., Tsukamoto, I., Mifune, M., Saito, Y
  • Journal Title: Chem. Pharm. Bull 55(3) Pages: 500-502
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Peroxidase-like catalytic activity of Mn^<3+>-octabromo-tetrakis-(4-sulfophenyl)porphine on linoleate hydroperoxide and its analytical application (2007)
  • Author(s): Mifune, M., Kamiguchi, H., Tai, T., Kuremoto, S., Yamamoto, M., Tsukamoto, I., Saito, M., Kitamura, Y., Saito, Y
  • Journal Title: Talanta 71(1) Pages: 456-461
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Evaluation of the State of Active Ingredients in Pharmaceutical Preparations Using Fourier Transform-Raman Difference Spectra (2006)
  • Author(s): Mifune, M., Iwasaki T., Kozaki Y., Tsukamoto I., Saito M., Kitamura, Y., Yamaguchi T., Saito Y.
  • Journal Title: Yakugaku Zasshi 126(12) Pages: 1363-1367
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Standard Infrared Absorption Spectrum of Betaine and Optimal Conditions for its Measurement (2006)
  • Author(s): Kitamura, Y., Iwasaki T., Saito, M., Mifune, M., Saito, Y., Sato, K., Yomota C. and Tanamoto K,
  • Journal Title: J. Food Hygienics Society of Japan 47(5) Pages: 232-236
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Effect of octabromination of a tetrakis (4-crboxyphenyl) porphine derivative bound to silica gels on HPLC retention behaviors of polyaromatic hydrocarbons (2006)
  • Author(s): Kitamura Y., Kawata K., Tanaka K., Furuyashiki Y., Mifune M., Tsukamoto I., Saito M., Haginaka J., Saito Y.
  • Journal Title: Talanta. 69(5) Pages: 1260-1264
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Peroxidase-like catalytic activity of Mn- and Fe-tetrakis (4-carboxyphenyl)-porphines bound to aminopropyl-glass bead in oxidative reaction of heterocyclicamines (2006)
  • Author(s): Kitamura Y., Mifune M., Hino D., Yokotani S., Saito M., Tsukamoto I., Iwado A., and Saito Y.
  • Journal Title: Talanta. 69(1) Pages: 43-47
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Evaluation of the State of Active Ingredients in Pharmaceutical Preparations Using Fourier Transform-Raman Difference Spectra (2006)
  • Author(s): Mifune, M., Iwasaki, T., Kozaki, Y., Tsukamoto, I., Saito, M., Kitamura, Y., Yamaguchi, T., Saito, Y
  • Journal Title: YAKUGAKU ZASSHI 126(12) Pages: 1363-1367
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Standard Infrared Absorption Spectrum of Betaine and Optimal Conditions for its Measurement (2006)
  • Author(s): Kitamura, Y., Iwasaki, T., Saito, M., Mifune, M., Saito, Y., Sato, K., Yomota, C., Tanamoto, K
  • Journal Title: J. Food Hygienics Society of Japan 47(5) Pages: 232-236
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effect of octabromination of a tetrakis(4-crboxyphenyl)porphine derivative bound to silica gels on HPLC retention behaviors of polyaromatic hydrocarbons (2006)
  • Author(s): Kitamura, Y., Kawata, K., Tanaka, K., Furuyashiki, Y., Mifune, M., Tsukamoto, I., Saito, M., Haginaka, J., Saito, Y
  • Journal Title: Talanta 69(5) Pages: 1260-1264
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Peroxidase-like catalytic activity of Mn- and Fe-tetrakis-(4-carboxyphenyl)porphines bound to aminopropyl-glass bead in oxidative reaction of heterocyclic amines (2006)
  • Author(s): Kitamura, Y., Mifune, M., Hino, D., Yokotani, S., Saito, M., Tsukamoto, I., Iwado, A., Saito, Y
  • Journal Title: Talanta 69(1) Pages: 43-47
  • Description: 「研究成果報告書概要(欧文)」より
• [Remarks] 「研究成果報告書概要(和文)」より
  • URL: http://www.pharm.okayama-u.ac.jp/lab/bunseki/index.html