2007 Fiscal Year Final Research Report Summary
Development of Evaluation system of Drug Metabolism Ability for Personalized Medicine using Environmentally Responsive Polymers
| Project/Area Number |
18590038
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyoritsu University of Pharmacy |
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Principal Investigator |
KANAZAWA Hideko Kyoritsu University of Pharmacy, Pharmacy, Professor (10240996)
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| Project Period (FY) |
2006 – 2007
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| Keywords | poly(N-isopropylacrylamide) / temperature-responsive chromatography / cytochrome P450 / environmentally responsive / personalized medicine / therapeutic drug monitoring |
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| Research Abstract |
Hepatic drug oxidation is a major source of interindividual variations in drug pharmacokinetics and therapeutic response. In vitro cytochrome P450 assays are used in metabolism studies in support of early phases of drug discovery to investigate, e. g., metabolic stability, enzyme inhibition and induction by new chemical entities. In this study, we investigated the separation of probe substrates of cytochrome P450 using temperature-responsive chromatographic system. Temperature-dependent resolution of 6 kinds of probe substrates which commonly used by the pharmaceutical industry to study in vitro drug interactions was achieved using only an isocratic aqueous solution as a mobile phase. The separation of probe drugs and its metabolites was also achieved by temperature changes without any changes in the mobile phase. In this system, the simultaneous separation of probe drugs and its metabolites was achieved by using only an aqueous solution as a mobile phase. Moreover, therapeutic drug monitoring of antiepileptic drugs with direct plasma injection without any pretreatment was achieved by the proposed system using only water as a mobile phase. This system is simple, convenient and expects to be useful for drug analysis in clinical field for personalized medicine.
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[Journal Article] Aqueous Chromatographic system for Separation of Biomolecules using Thermoresponsive Polymer Modified Stationary Phase2008
Author(s)
H., Kanazawa, M., Nishikawa, A., Mizutani, C., Sakamoto, Y., Morita-Murase, Y., Nagata, A., Kikuchi and T., Okano
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Journal Title
J. Chromatogr. A 1191
Pages: 157-161
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Analysis of Melatonin using pH- and Temperature-responsive Aqueous Chromatographic System2007
Author(s)
E., Ayano, Y., Suzuki, M., Kanezawa, C., Sakamoto, Y., Morita, Y., Nagata, H., Kanazawa, A., Kikuchi, T., Okano
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Journal Title
J Health Sci 53
Pages: 146-150
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A developed determination of midazolam and 1'-hydroxymidazolam in plasma by liquid chromatography-mass spectrometry : Application to human pharmacokinetic study for measurement of CYP3A activity2007
Author(s)
M., Shimizu, T., Uno, H., Tamura, H., Kanazawa, I., Murakami, T., Tateishi
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Journal Title
J. Chromatogr. B Analyt. Technol. Biomed. Life Sci 847
Pages: 275-81
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Pharmacokinetics of fentanyl after single intravenous injection and constant rate infusion in dogs2006
Author(s)
T., Sano, R., Nishimura, H., Kanazawa, E., Igarashi, Y., Nagata, M., Mochizuki, N., Sasaki
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Journal Title
Vet Anaesth Analg 33
Pages: 266-73
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Increased F2-Isoprostane Levels in the Rat Brain and Plasma Caused by Oxidative Stress and Aging, and Inhibitory Effect of Vitamin E2006
Author(s)
T., Nishio, R., Miyadera, R., Sakai, K., Abe, H., Kanazawa, K., Fukui, S., Urano
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Journal Title
J. Clin. Biochem. Nutr 38
Pages: 161-166
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Study of temperature-responsibility on the surfaces of a thermo-responsive polymer modified stationary phase2006
Author(s)
E., Ayano, Y., Okada, C., Sakamoto, H., Kanazawa, A., Kikuchi, T., Okano
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Journal Title
J. Chromatogr. A 1119
Pages: 51-57
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Aqueous chromatography system using pH-and temperature-responsive stationary phase with ion-exchange groups2006
Author(s)
E., Ayano, K., Nambu, C., Sakamoto, H., Kanazawa, A., Kikuchi, T., Okano
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Journal Title
J. Chromatogr. A 1119
Pages: 58-65
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Simultaneous determination of catechins and procyanidins in bottled tea drinks by LC/MS2006
Author(s)
I., Murakami, T., Nakamura, Y., Ishibashi, R., Shibuya, E., Ayano, Y., Morita-Murase, Y., Nagata, H., Kanazawa
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Journal Title
CHROMATOGRAPHY 27
Pages: 19-25
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Temperature-responsive stationary phase utilizing a polymer of proline derivative for hydrophobic interaction chromatography using an aqueous mobile phase2006
Author(s)
H., Kanazawa, E., Ayano, C., Sakamoto, R., Yoda, A., Kikuchi, T., Okano
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Journal Title
J. Chrmatogr. A 1106
Pages: 152-158
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Thermoresponsive polymer brush interfaces ; effect of chain length for controlled cell adhesion / detachment behavior2007
Author(s)
Aya, Mizutani, Akihiko, Kikuchi, Jun, Kobayashi, Yoshikatsu, Akiyama, Masayuki, Yamato, Hideko, Kanazawa, Teruo, Okano
Organizer
Tissue Engineering International & Regenerative Medicine Society Asia-Pacific Chapter Meeting 2007
Place of Presentation
Tokyo, Japan
Year and Date
20071200
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Thermoresponsive polymer brush interfaces ; surface characterization for controlled cell adhesion2007
Author(s)
Aya, Mizutani, Akihiko, Kikuchi, Jun, Kobayashi, Yoshikatsu, Akiyama, Masayuki, Yamato, Hideko, Kanazawa, Teruo, Okano
Organizer
Society for biomaterials 2007 annual meeting
Place of Presentation
Chicago, Illinois
Year and Date
20070400
Description
「研究成果報告書概要(欧文)」より
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