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2007 Fiscal Year Final Research Report Summary

Tissue specific regulation of BMAL1 gene by dietary fat

Research Project

Project/Area Number 18590077
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionNihon University

Principal Investigator

SHIMBA Shigeki  Nihon University, College of Pharmacy, Department of Health Science, Associate Professor (20287668)

Project Period (FY) 2006 – 2007
KeywordsBMAL1 / adipocytes / metabolic syndrome
Research Abstract

Adipocytes play essential metabolic roles not only serving as massive energy reserves but also secreting hormones and cytokines that regulate metabolic activities. The link between metabolic activity in adipocytes and circadian rhythm has long been studied ; e.g. glucose and lipid homeostasis are well known to exhibit circadian variation. More recently, we reported that white adipose tissue contains functional molecular clock and that expression of several adipocytokines, including leptin, and plasminogen activator inhibitor-1 display circadian rhythm (Aoyagi, et. al. JHS 2005). Therefore, molecular clock may play important roles in the regulation of metabolic activity in adipocytes. BMAL1, a master component of circadian rhythm, is abundantly expressed in mature adipocytes in vivo and in vitro. This transcription factor regulates the expression of several factors involved in lipogenesis in adipocytes such as SREBP-1 (Shimba, et. al. PNAS 2005). Also, BMAL1 KO mice show phenotype of si … More gnificant reduction of adipose tissue, while the liver in KO mice is as intact as that in wild type. These characteristics of BMAL1 lead us to study the adipocytes-specific roles of this factor. In this study, we first examine the effects of dietary fat on BMAL1 expression and functions in mature adipocytes. The challenge to high fat diet results in significant induction of Bmal1 mRNA in mice adipose tissue. Interestingly, the induction of circadian gene by high-fat diet is observed only in adipose tissue, but not in other tissues tested. This adipose tissue specific induction of Bmal1 expression accompanied with enhanced expression of its targets genes such as Per and PAI-1. Tb determine whether dietary fats have a direct impact on Bmal1 expression, cultured adipocytes were treated with various saturated, unsaturated, and trans fatty acids and examined levels of Bmal1 mRNA One of the fatty acids significantly increased Bmal1 mRNA level in cultured adipocytes but not in primary hepatocytes. Inhibition of the fatty acid metabolism diminished the effect on Bmal1 expression. Tentative metabolites of the fatty acid increased Bmal1 mRNAlevel Finally, the fatty acid-treatment induced cyclic expression of circadian genes in cultured adipocytes. These results suggest that dietary fatty acids can modulate adipocytes specific circadian rhythm machinery. Less

  • Research Products

    (18 results)

All 2008 2007

All Journal Article (6 results) (of which Peer Reviewed: 2 results) Presentation (12 results)

  • [Journal Article] Comparative study of hydrogen peroxide-and 4-hydroxy-2-nonenal-induced cell death in HT22 cells2008

    • Author(s)
      Ishimura, Atsushi
    • Journal Title

      Neurochem.Int. 52

      Pages: 776-785

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Comparative study of hydrogen peroxide- and 4-hydroxy-2-nonenal-induced cell death in HT22 cells2008

    • Author(s)
      Ishimura, A., Ishige, K., Taira, T., Shimba, S., Ono, SI., Ariga, H., Tezuka, M., Ito, Y
    • Journal Title

      Neurochem. Int 52

      Pages: 776-785

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] メタボリックシンドローム発症への時計遺伝子の関与2007

    • Author(s)
      榛葉 繁紀
    • Journal Title

      Clinical Neuroscience(中外医学社) 25

      Pages: 1144-1146

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Characterization of the Molecular Clock in Mouse Peritoneal Macrophages2007

    • Author(s)
      Hayashi, M., Shimba, S., Tezuka, M
    • Journal Title

      Biol.Pharm.Bull. 30

      Pages: 49-54

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Molecular clock is involved in the onset of the metabolic syndrome2007

    • Author(s)
      Shimba, S
    • Journal Title

      Clinical Neuroscience 25

      Pages: 1144-1146

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Characterization of the Molecular Clock in Mouse Peritoneal Macrophages2007

    • Author(s)
      Hayashi, M., Shimba, S.*, Tezuka, M
    • Journal Title

      Biol. Pharm. Bull 29

      Pages: 49-54

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 脂肪細胞においてアラキドン酸は時計遺伝子BMAL1の発現を誘導する2008

    • Author(s)
      村林 紀雄, 榛葉 繁紀, 他
    • Organizer
      日本薬学会第128年会
    • Place of Presentation
      横浜
    • Year and Date
      2008-03-27
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Dietary fat regulates Bmal1 expression in adipocytes2008

    • Author(s)
      Murabayashi, N., Shimba, S., Tezuka, M
    • Organizer
      The annual meeting of the pharmaceutical. society of JAPAN
    • Place of Presentation
      Yokohama
    • Year and Date
      2008-03-27
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] アラキドン酸による脂肪細胞特異的な時計遺伝子BMAL1の発現誘導2007

    • Author(s)
      村林 紀雄, 榛葉 繁紀, 他
    • Organizer
      第30回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-12
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Dietary fat regulates Bmal1 expression in adipocytes2007

    • Author(s)
      Murabayashi, N., Shimba, S., Tezuka, M
    • Organizer
      The annual meeting of the molecular biology society of Japan
    • Place of Presentation
      Yokohama
    • Year and Date
      2007-12-12
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] アラキドン酸による脂肪細胞特異的な時計遺伝子BMAL1の発現誘導2007

    • Author(s)
      村林 紀雄, 榛葉 繁紀, 他
    • Organizer
      第14階日本時間生物学会学術大会
    • Place of Presentation
      東京
    • Year and Date
      2007-11-07
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 時計遺伝子BMAL1の脂肪細胞特異的な機能ならびに発現制御機構2007

    • Author(s)
      榛葉 繁紀
    • Organizer
      第14階日本時間生物学会学術大会
    • Place of Presentation
      東京
    • Year and Date
      2007-11-07
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Dietary fat regulates Bmal1 expression in adipocytes2007

    • Author(s)
      Murabayashi, N., Shimba, S., Tezuka, M
    • Organizer
      The annual meeting of Japanese society for chronobiology
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-11-07
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] BMAL1 regulates adipocytes functions2007

    • Author(s)
      Shimba, S
    • Organizer
      The annual meeting of Japanese society for chronobiology
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-11-07
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 糖尿病発症のリスクファクターとしての間食ならびに夜食の影響2007

    • Author(s)
      榛葉 繁紀
    • Organizer
      第5回日本セルフメディケーション学会
    • Place of Presentation
      船橋
    • Year and Date
      2007-10-14
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Midnight snack is a risk factor of diabetes2007

    • Author(s)
      Shimba, S
    • Organizer
      The annual meeting of the self-medication society
    • Place of Presentation
      Funabashi
    • Year and Date
      2007-10-14
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] アラキドン酸による脂肪細胞特異的な時計遺伝子BMAL1の発現誘導2007

    • Author(s)
      村林 紀雄, 榛葉 繁紀, 他
    • Organizer
      第12回アディポサイエンス研究会シンポジウム
    • Place of Presentation
      大阪
    • Year and Date
      2007-08-18
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Dietary fat regulates Bmal1 expression in adipocytes2007

    • Author(s)
      Murabayashi, N., Shimba, S., Tezuka, M
    • Organizer
      Symposium on adiposcience
    • Place of Presentation
      Osaka
    • Year and Date
      2007-08-18
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-02-04  

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