2007 Fiscal Year Final Research Report Summary
Development of Recovering Equipment of Free Oligosaccharides Chemically Converted from Fluorescent Labeled Glycans by Hydrogen Peroxide and Its Application of Two-dimensional Liquid Chromatography
| Project/Area Number |
18590086
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Kinki University |
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Principal Investigator |
SUUKI Shigeo Kinki University, School of Pharmacy, Professor (00154542)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Glycoprotein glycans / Reductive amination reaction / Glycomics / Flurescent labeling / 2-aminopyridine / Ethyl ρ-aminobenzoate / 7-Amino-4-methylcoumarin / ρ-aminobenzamide |
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| Research Abstract |
This study examined chemical regeneration of free oligosaccharides from their Fuorescent derivatives prepared by reductive amination with various aromatic amines. Maltose derivatives of ethyl 4-aminobenzoate (p-ABEE), 2-aminobenzonitrile (o-ABN), 4-aminobemonitrile (p-ABN), 7-amino-4-methylcoumarin (AMC), 2-aminobenzoic acid (o-ABA), 2-aminobenzamide (o-ABAD), 2-aminopyridine (AP), and 8-aminonaphthalene-1,3,6-trisulfonare (ANTS) were incubated at 30℃ with an aqueous solution of hydrogen peroxide/acetic acid. Recoveries of maltose from p-ABEE, p-ABN, and AMC derivatives were fairly good and gave approximately 90% of maltose. Recoveries of maltose from its o-substituted aniline (o-ABA, o-ABAD, and o-ABN) derivatives were 5-40%, but maltose was unrecoverable from AP and ANTS derivatives. Nevertheless, prior treatment of an AP derivative with cyanogen bromide enabled the regeneration of maltose in high yields. As an application, p-ABEE-labeled N-glycans from some glycoproteins separated on an amide column were identified by converting peak components to their AP derivatives via free saccharides and following mapping by reversed phase chromatography.
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[Presentation] 糖質科学と分析化学2007
Author(s)
鈴木 茂生
Organizer
第26回日本応用糖質科学会支部会
Place of Presentation
神戸大学
Year and Date
2007-10-19
Description
「研究成果報告書概要(和文)」より
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