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2007 Fiscal Year Final Research Report Summary

Research for outer membrane remodeling in Salmonella enterica

Research Project

Project/Area Number 18590091
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionDoshisha Women's College of Liberal Arts

Principal Investigator

KAWASAKI Kiyoshi  Doshisha Women's College of Liberal Arts, Faculty of Pharmaceutical Sciences, Associate Professor (60270641)

Project Period (FY) 2006 – 2007
KeywordsSalmonella enterica / endotoxin / lipopolysaccharide / lipase / deacylation / enzyme / regulation / point-mutation
Research Abstract

Salmonella enterica serovar Typhimurium modifies its lipopolysaccharide (LPS), including the lipid A portion, in response to changes in its environment including host tissues. The lipid A 3-O-deacylase PagL, the expression of which is promoted under the host-mimetic environment, exhibits latency in S. enterica ; deacylation of lipid A is not usually observed in vivo despite the expression of the outer membrane protein PagL. In contrast, PagL does not exhibit latency in S. enterica pmrA and pmrE mutants, both of which are deficient in the aminoarabinose-based modification of lipid A, indicating that aminoarabinose-modified LPS species were involved in the latency. In order to analyze the machinery for PagL's repression, we generated PagL mutants in which an amino acid residue located at four extracellular loops was replaced with alanine. Apparent lipid A 3-O-deacylation was observed in S. enterica expressing the several PagL mutants, but not in S. enterica expressing wild-type PagL, suggesting that the point mutations released PagL from the latency. In addition, the mutations did not affect lipid A 3-O-deacylase activity of PagL in a S. enterica pmrA mutant or in E. coli BL21(DE3). These results, taken together, indicate that specific amino acid residues located at extracellular loops of PagL are involved in the recognition of aminoarabinose-modified LPS. Furthermore, S enterica expressing the PagL mutants showed apparent growth arrest at 43℃ compared with S. enterica expressing wild-type PagL, indicating that the latency of PagL is important for bacterial growth.

  • Research Products

    (20 results)

All 2008 2007 2006

All Journal Article (6 results) (of which Peer Reviewed: 3 results) Presentation (14 results)

  • [Journal Article] Release of lipopolysaccharide deacylase PagL from latency compensates for a lack of lipopolysaccharide aminoarabinose modification-dependent resistance to antimicrobial peptide polymyxin B in Salmonella enterica2007

    • Author(s)
      K.Kawasaki, 他2名
    • Journal Title

      J.Bacteriol. 189

      Pages: 4911-4919

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Release of lipopolysaccharide deacylase PagL from latency compensates for a lack of lipopolysaccharide aminoarabinose modification-dependent resistance to antimicrobial peptide polymyxin B in Salmonella enterica.2007

    • Author(s)
      K. Kawasaki, et. al.
    • Journal Title

      J. Bacteria 189

      Pages: 4911-4919

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] サルモネラ菌の外膜リモデリングと宿主応答2006

    • Author(s)
      川崎 清史
    • Journal Title

      薬学雑誌 126

      Pages: 1227-1234

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Regulatory roles for MD-2 and TLR4 in ligand-induced receptor clustering2006

    • Author(s)
      M.Kobayashi, 他9名
    • Journal Title

      J.Immunol. 176

      Pages: 6211-6218

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Regulatory roles for MD-2 and TLR4 in ligand-induced receptor clustering.2006

    • Author(s)
      M. Kobayashi, et. al.
    • Journal Title

      J. Immunol 176

      Pages: 6211-6218

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Outermembrane remodeling of Salmonella typhimurium and host innate immunity2006

    • Author(s)
      K. Kawasaki
    • Journal Title

      YAKUGAKU ZASSHI 126

      Pages: 1227-1234

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] サルモネラ菌のLPS脱アシル化酵素は外膜のアミノアラビノース修飾を認識する2008

    • Author(s)
      眞鍋 貴行、川崎 清史
    • Organizer
      第81回日本細菌学会総会
    • Place of Presentation
      京都
    • Year and Date
      20080300
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] LPS deacylase recognize membrane axninoarabinose modification in S. enterica.2008

    • Author(s)
      T. Manabe, K Kawasaki
    • Organizer
      81th meetings of Japan Bacteriology Society
    • Place of Presentation
      Kyoto
    • Year and Date
      20080300
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] LPS脱アシル化酵素の活性調節に関与するアミノ酸残基の同定2007

    • Author(s)
      眞鍋 貴行, 他2名
    • Organizer
      第80回日本生化学会大会
    • Place of Presentation
      横浜
    • Year and Date
      20071200
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Identification of amino acid residues involved in PagL repression2007

    • Author(s)
      T. Manabe, et. al.
    • Organizer
      BMB2007
    • Place of Presentation
      Yokohama
    • Year and Date
      20071200
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] サルモネラ菌の抗菌ペプチド感受性のLPS脱アシル化による調節2007

    • Author(s)
      川崎 清史、西島 正弘
    • Organizer
      第13回日本エンドトキシン研究会
    • Place of Presentation
      鹿児島
    • Year and Date
      20071000
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Modulation of susceptibility of antimicrobial peptide in S. typhimurium by lipid A deacylation2007

    • Author(s)
      K. Kawasaki, M Nishijima
    • Organizer
      13th meetings of Japan Endotoxin Society
    • Place of Presentation
      Kagoshima
    • Year and Date
      20071000
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] サルモネラ菌のリポ多糖修飾の機能と制御2007

    • Author(s)
      川崎 清史
    • Organizer
      第19回微生物シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      20070900
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Regulation and functions of lipopolysaccharide modifications in S. enterica2007

    • Author(s)
      K. Kawasaki
    • Organizer
      19th symposium on Microbiology
    • Place of Presentation
      Tokyo
    • Year and Date
      20070900
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] LipidA修飾酵素PagLの活性抑制に関与するアミノ酸残基の同定2007

    • Author(s)
      眞鍋 貴行, 他2名
    • Organizer
      日本薬学会第127年会
    • Place of Presentation
      富山
    • Year and Date
      20070300
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Identification of amino acid residues involved in PagL repression2007

    • Author(s)
      T. Manabe, et. al.
    • Organizer
      127th meeting of Japan Pharmaceutical Society
    • Place of Presentation
      Toyama
    • Year and Date
      20070300
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] サルモネラ菌抗菌ペプチド感受性のLPS脱アシル化による制御2006

    • Author(s)
      川崎 清史, 他2名
    • Organizer
      第48回日本脂質生化学会
    • Place of Presentation
      東京
    • Year and Date
      20060600
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Lipopolysaccharide deacylation controls resistance to cationic antimicrobial peptide polymyxin B in Salmonella typhimurium2006

    • Author(s)
      Kiyoshi Kawasaki, 他2名
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      京都
    • Year and Date
      20060600
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Regulation of susceptibility of antimicrobial peptide in S. typhimurium by lipid A deacylation2006

    • Author(s)
      K. Kawasaki, et. al.
    • Organizer
      48th JCBL
    • Place of Presentation
      Tokyo
    • Year and Date
      20060600
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Lipopolysaccharide deacylation controls resistance to cationic antimicrobial peptide polymyxin B in Salmonella typhimurium2006

    • Author(s)
      K. Kawasaki, et. al.
    • Organizer
      20th IUBMB
    • Place of Presentation
      Kyoto
    • Year and Date
      20060600
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-02-04  

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