A study on lipid droplet biogenesis in mammalian cells

2009 Fiscal Year Final Research Report

• Project/Area Number: 18590092
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: FUKASAWA Masayoshi National Institute of Infectious Diseases, 細胞化学部, 室長 (20291130)
• Project Period (FY): 2006 – 2009
• Keywords: 生化学 / 脂肪滴

Research Abstract

Lipid droplets are cellular lipid storage organelles involved not only in lipid homeostasis but also in a variety of diseases. However, little is known about cellular factors involved in the biogenesis of lipid droplets. In this study, we performed proteomic profiling of lipid droplet proteins in human liver cell lines and identified more than 80 proteins by 1D-SDSPAGE/MALDI-TOF mass spectrometry (MS) or direct nano-flow liquid chromatography-MS/MS. I also found that acetyl-CoA carboxylase 1 has an essential role in lipid droplet biogenesis in cultured mammalian cells.

Research Products

• [Journal Article] Cellular lipid droplets and hepatitis C virus life cycle (2010)
  • Author(s): Fukasawa M.
  • Journal Title: Biol. Pharm. Bull. 33(3) Pages: 355-359
  • Peer Reviewed
• [Journal Article] Proteomic profiling of lipid droplet proteins in hepatoma cell lines expressing hepatitis C virus core protein. (2006)
  • Author(s): Sato, S., Fukasawa, M., Yamakawa, Y., Natsume, T., Suzuki, T., Shoji, I., Aizaki, H., Miyamura, T., Nishijima, M.
  • Journal Title: J. Biochem. 139 Pages: 921-930
  • Peer Reviewed
• [Journal Article] Enhancement of de novo fatty acid biosynthesis in hepatic cell line Huh7 expressing hepatitis C virus core protein. (2006)
  • Author(s): Fukasawa M, Tanaka Y, Sato S, Ono Y, Nitahara-Kasahara Y, Suzuki T, Miyamura T, Hanada K, Nishijima M.
  • Journal Title: Biol. Pharm. Bull. 29(9) Pages: 1958-61
  • Peer Reviewed
• [Presentation] キサントン誘導体による脂肪滴蓄積阻害活性はacetyl-CoA carboxylaseの阻害によるのだろうか? (2008)
  • Author(s): 松田大介、小林翔、深澤征義、西島正弘、花田賢太郎、司書毅、供田洋
  • Organizer: 第50回日本脂質生化学会
  • Place of Presentation: 徳島
  • Year and Date: 20080605-20080606
• [Presentation] 供田洋ACC1阻害剤による脂肪滴蓄積阻害活性 (2008)
  • Author(s): 小林翔、松田大介、深澤征義、西島正弘、花田賢太郎、司書毅
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
  • Year and Date: 20080326-20080328
• [Presentation] C型肝炎ウイルスコア蛋白質発現培養肝細胞における脂肪酸生合成の上昇 (2007)
  • Author(s): 深澤征義, 田中康仁、佐藤慈子、笠原優子, 鈴木哲朗, 宮村達男, 花田賢太郎, 西島正弘
  • Organizer: 日本薬学会第127年会
  • Place of Presentation: 富山
  • Year and Date: 20070328-20070330
• [Presentation] Enhancement of de novo fatty acid biosynthesis in hepatic cell line Huh7 expressing hepatitis C virus core protein (2006)
  • Author(s): 深澤征義、田中康仁、小野祐仁、佐藤慈子、鈴木哲朗、宮村達男、花田賢太郎、西島正弘
  • Organizer: The 13th International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: Cairns, Australia
  • Year and Date: 20060827-20060831
• [Presentation] Isolation of a mammalian cell mutant defective in lipid droplet biogenesis (2006)
  • Author(s): 深澤征義、西島正弘
  • Organizer: The 20th IUBMB International Congress of Biochemistry and Molecular Biology/11th FAOBMB Congress
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20060618-20060623