Relationship between xenobiotic toxicity and metabolisn in individuals

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 18590116
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental pharmacy
• Research Institution: Okayama University
• Principal Investigator: HANIOKA Nobumitsu Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Associate Professor (70228518)
• Co-Investigator(Kenkyū-buntansha): NARIMATSU Shizuo Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Professor (20113037)
• Project Period (FY): 2006 – 2007
• Keywords: Environmental pollutants / Drug-metabolizing enzymes / UDP-glucuronosyltransferase / β-nanhthoflavone / HepG2 cells / UGT1A1 / UGT1A6 / UGTIA9

Research Abstract

Since glucuronidation is an important metabolic reaction for xenobiotic elimination, information on the induction or suppression of UDP-glucuronosyltransferase (UGT) enzymes at the isoform level is beneficial in drug therapy and the toxicological assessment of environmental chemicals. In this study, we focused on UGT1A isoforms (UGT1A1, UGT1A6 and UGT1A9), mainly expressed in the human liver, and examined the inducibility of UGT1As by β-naphthoflavone (BNF) in human hepatoma HepG2 cells. The cells were pretreated for 72 h with BNF at concentrations of 25, 50 and 100 μM. 7-Ethyl-10-hydroxycamptothecin (SN-38) glucuronidation, used as a probe for UGT1A1, showed sigmoidal kinetics with a Hill coefficient (n) of 1.2-1.3 in control and BNF-pretreated HepG2 cells. The V_<max> values were significantly increased 3.6- to 4.3-fold by BNF, whereas there was no significant change in the S_<50> values by BNF at any concentration examined. On the other hand, 4-methylumbelliferone (4-MU) glucuronida tion as a probe for UGT1A6 and UGT1A9 in the control and BNF-pretreated HepG2 cells exhibited a biphasic kinetic pattern Although K_<ml> values for the low-K_m phase were similar between the control and BNF-pretreated HepG2 cells, K_<m2> values for the high-K_m phase of BNF-pretreated liepG2 cells were reduced to 54-69% of control HepG2 cells. The values of V_<max> and V_<max2> for the low- and high-K_m phases, respectively, were significantly increased 1.9- to 2.6-fold by BNF at 25 and/or 50μM but not 100 μM With respect to V_<max> (V_<max1> and V_<max2>) and V_<max>/K_m (V_<max1>/K_<m1> and V_<max2>/K_<m2>), the values were significantly increased 2.0- to 3.2-fold by BNF at all concentrations examined. Furthermore, real-time reverse transcription polymerase chain reaction (RT-PCR) using TaqMan probes demonstrated that BNF concentration-dependently induced mRNA levels of UGTIA1 but not UGT1A6 or UGT1A9 in HepG2 cells (1.3- to 6.0-fold). These results suggest that the inducibility of UGT1A isoforms in HepG2 cells by BNF is different from other aryl hydrocarbon receptor (AhR) agonists previously reported, and should provide useful information for the prediction of drug-drug interactions and toxicological assessment of environmental chemicals.

Research Products

• [Journal Article] Interaction of bisphenol A with human UDP-glucuronosyltransferase 1A6 enzyme (2008)
  • Author(s): Hanioka, N., Takeda, Y., Tanaka-Kagawa, T., Hayashi, K., Jinno, H., and Narimatsu, S.
  • Journal Title: Environ. Toxicol. (印刷中)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Influence of CYP2C19^*18 and CYP2C19^*19 alleles on omeprazole 5-hydroxylation: in vitro functional analysis of recombinant enzymes expressed in Saccharomyces cerevisiae (2008)
  • Author(s): Hanioka, N., Tsuneto, Y., Saito, Y., Maekawa, K., Sawada, J., and Narimatsu, S.
  • Journal Title: Basic Clin. Pharmacol. Toxicol. (印刷中)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Functional characterization of two novel CYP2C19 variants (CYP2C19^*18 and CYP2C19^*19) found in a Japanese population (2007)
  • Author(s): Hanioka, N., Tsuneto, Y., Saito, Y., Sumada, T., Maekawa, K., Saito, K., Sawada. J., and Narimatsu, S.
  • Journal Title: Xenobiotica 37(4) Pages: 342-355
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Functional characterization of human and cynomolgus monkey cytochrome P450 2E1 enzymes (2007)
  • Author(s): Hanioka, N., Yamamoto, M., Iwabu, H., Jinno, H., Tanaka-Kagawa, T., Naito, S., Shimizu, T., Masuda, K., Katsu, T., and Narimatsu, S.
  • Journal Title: Life Sci. 81(19-20) Pages: 1436-1445
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Functional characterization of two novel CYP2C19 variants (CYP2C19*18 and CYP2C19*19) found in a Japanese population (2007)
  • Author(s): Hanioka, N., Tsuneto, Y., Saito, Y., Sumada, T., Maekawa, K., Saito, K., Sawada, J., Narimatsu, S
  • Journal Title: Xenobiotica 37 (4) Pages: 342-355
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Functional characterization of human and cynomolgus monkey cytochrome P450 2E1 enzymes (2007)
  • Author(s): Hanioka, N., Yamamoto, M., Iwabu, H., Jinno, H., Tanaka-Kagawa, T., Naito, S., Shimizu, T., Masuda, K., Katsu, T., Narimatsu, S
  • Journal Title: Life Sci 81(19-20) Pages: 1436-1445
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Catalytic roles of CYP2D6.10 and CYP2D6.36 enzymes in mexiletine metabolism: in vitro functional analysis of recombinant proteins expressed in Saccharomyces cerevisiae (2006)
  • Author(s): Hanioka, N., Okumura, Y., Saito, Y., Hichiya, H., Soyama, A., Saito, K., Ueno, K., Sawada, J., and Narimatsu S.
  • Journal Title: Biochem. Pharmacol. 71(9) Pages: 1386-1395
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Inducibility of UDP-glucuronosyltransferase 1As by β-naphthoflavone in HepG2 cells (2006)
  • Author(s): Hanioka, N., Obika, N., Nishimura, M., Jinno, H., Tanaka-Kagawa, T., Saito, K., Kiryu, K., Naito, S., and Narimatsu, S.
  • Journal Title: Food Chem. Toxicol. 44(8) Pages: 1251-1260
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Functional characterization of human and cynomolgus monkey UDP-glucuronosyltransferase 1A6 enzymes (2006)
  • Author(s): Hanioka, N., Takeda, Y., Jinno, H., Tanaka-Kagawa, T, Naito, S., Koeda, A., Shimizu, T., Nomura, M., and Narimatsu, S.
  • Journal Title: Chem. Biol. Interact. 164(1-2) Pages: 136-145
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Catalytic roles of CYP2D6.10 and CYP2D6.36 enzymes in mexiletine metabolism : in vitro functional analysis of recombinant proteins expressed in Saccharoniyces cerevisiae (2006)
  • Author(s): Hanioka, N., Okumura, Y., Saito, Y., Hichiya, H., Soyama, A., Saito, K., Ueno, K., Sawada, J., Narimatsu, S
  • Journal Title: Biochem. Pharmacol 71 (9) Pages: 1386-1395
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Inducibility of UDP-glucuronosyltransferase lAs by 13-naphthoflavone in HeoG2 cells (2006)
  • Author(s): Hanioka, N., Obika, N., Nishimura, M., Jinno, H., Tanaka-Kagawa, T., Saito, K., Kiryu, K., Naito, S., Narimatsu, S
  • Journal Title: Food Chem. Toxicol 44(8) Pages: 1251-1260
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Functional characterization of human and cynomolgus monkey UDP-glucuronosyltransferase lA6 enzymes (2006)
  • Author(s): Haitioka, N., Takeda, Y., Jinno, H., Tanaka-Kagawa, T., Naito, S., Koeda, A., Shimizu, T., Nomura, M., Narimatsu, S
  • Journal Title: Chem. Biol. Interact 164(1-2) Pages: 136-145
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Interaction of bisphenol A with human UDP-glucuronosyltransferase 1A6 enzyme
  • Author(s): HanioIca, N., Takeda, Y., Tanaka-Kagawa, T., Hayashi, K., Jinno, H., Narimatsu, S
  • Journal Title: Environ. Toxicol (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Influence of CYP2C19*18 and CYP2C19*19 alleles on omeprazole 5-hydroxylation : in vitro functional analysis of recombinant enzymes expressed in Saccharomyces cerevisiae
  • Author(s): Hanioka, N., Tsuneto, Y., Saito, Y., Maekawa, K., Sawada, J., Narimatsu, S
  • Journal Title: Basic Clin. Pharmacol. Toxicol (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ヒトHNF4αを介した転写活性化に及ぼすSHPの影響 (2007)
  • Author(s): 中田晋太郎, 斎藤嘉朗, 佐伯真弓, 澤田純一, 埴岡伸光, 成松鎭雄
  • Organizer: 第46回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
  • Place of Presentation: 高知
  • Year and Date: 2007-11-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カニクイザルUGT2B20の酵素機能解析 (2007)
  • Author(s): 長岡憲次郎, 風盛大地, 埴岡伸光, 清水剛文, 内藤真策, 成松鎭雄
  • Organizer: 第46回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
  • Place of Presentation: 高知
  • Year and Date: 2007-11-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Effect of SHP on transcriptional activities mediated by human HNF4α (2007)
  • Author(s): Nakada, S., Saito, Y., Saeki, M., Sawada, J., Hanioka, N., Narimatsu, S
  • Organizer: 46th Annual Meeting of Chugoku-Shikoku Branch of the Pharmaceutical Society of Japan
  • Place of Presentation: Kochi, Japan
  • Year and Date: 2007-11-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Functional characterization of cynomolgus monkey UGT2B20 (2007)
  • Author(s): Nagaoka, K., Kazamori, D., Hanioka, N., Shimizu, T., Naito, S., Narimatsu, S
  • Organizer: 46th Annual Meeting of Chugoku-Shikoku Branch of the Pharmaceutical Society of Japan
  • Place of Presentation: Kochi, Japan
  • Year and Date: 2007-11-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ヒト乳癌細胞株MCF-7におけるUDP-グルクロン酸転移酵素の発現・誘導性 (2007)
  • Author(s): 岩部寛之, 埴岡伸光, 成松鎭雄
  • Organizer: フォーラム2007:衛生薬学・環境トキシコロジー
  • Place of Presentation: 大阪
  • Year and Date: 2007-11-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ビスフェノールAのグルクロン酸抱合反応に関与するヒトUGT分子種とその役割 (2007)
  • Author(s): 内藤尚徳, 埴岡伸光, 成松鎭雄
  • Organizer: フォーラム2007:衛生薬学・環境トキシコロジー
  • Place of Presentation: 大阪
  • Year and Date: 2007-11-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 常温揮散性ピレスロイド剤・Profluthrinの加水分解に関与するヒトCarboxylesteraseに関する研究 (2007)
  • Author(s): 香川(田中)聡子, 埴岡伸光, 松本拓, 徳永裕司, 成松鎭雄, 神野透人
  • Organizer: フォーラム2007:衛生薬学・環境トキシコロジー
  • Place of Presentation: 大阪
  • Year and Date: 2007-11-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Inducibility of UDP-glucuronosykransferases by β-naphthoflavone in human breast cancer MCF-7 cells (2007)
  • Author(s): Iwabu, H., Hanioka, N., Narimatsu, S
  • Organizer: Forum 2007 : Pharmaceutical Health Science and Environmental Toxicology
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2007-11-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Human UGT isoforms involved in the glucuronidation of bisphenol A (2007)
  • Author(s): Naito, T., Hanioka, N., Narimatsu, S
  • Organizer: Forum 2007 : Pharmaceutical Health Science and Environmental Toxicology
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2007-11-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Hydrolysis of profluthrin, the highly-volatile pyrethroid insecticides, by human carboxylesterases (2007)
  • Author(s): Tanaka-Kagawa, T., Hanioka, N., Matsumoto, T., Tokunaga, H., Narimatsu, S., Jinno, H
  • Organizer: Forum 2007 : Pharmaceutical Health Science and Environmental Toxicology
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2007-11-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Characterization of cynomolgus monkey UGT1A6:comparison with human UGT1A6 (2007)
  • Author(s): 埴岡伸光, 竹田友理, 神野透人, 香川(田中)聡子, 内藤真策, 清水剛文, 成松鎭雄
  • Organizer: 第8回国際薬物動態学会
  • Place of Presentation: 仙台
  • Year and Date: 2007-10-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Functional characterization of human and cynomolgus monkey cytochrome P450 2E1 enzymes (2007)
  • Author(s): 山本真紀, 岩部寛之, 米本麗, 増田和文, 勝孝, 埴岡伸光, 神野透人, 香川(田中)聡子, 内藤真策, 清水剛文, 成松鎭雄
  • Organizer: 第8回国際薬物動態学会
  • Place of Presentation: 仙台
  • Year and Date: 2007-10-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Functional characterization of human and cynomolgus monkey cytochrome P450 2E1 enzymes (2007)
  • Author(s): Yamanoto, M., Iwabu, H., Yonemoto, R., Masuda, K., Katsu, T., Hanioka, N., Jinno, H., Tanaka-Kagawa, T., Naito, S., Shimizu, T., Narimatsu, S
  • Organizer: 8th International ISSX Meeting
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2007-10-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Characterization of cynomolgus monkey UGT1A6 : comparison with human UGT1A6 (2007)
  • Author(s): Hanioka, N., Takeda, Y., Jinno, H., Tanaka-Kagawa, T., Naito, S., Shimizu, T., Narimatsu, S
  • Organizer: 8th International ISSX Meeting
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2007-10-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] カニクイザル及びヒトCYP2E1の機能解析 (2007)
  • Author(s): 山本真紀, 米本麗, 岩部寛之, 増田和文, 勝孝, 埴岡伸光, 香川(田中)聡子, 神野透人, 内藤真策, 清水剛文, 成松鎭雄
  • Organizer: 日本薬学会第127年会
  • Place of Presentation: 富山
  • Year and Date: 2007-03-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ヒト乳癌細胞株MCF-7における薬物代謝酵素の発現・誘導性 (2007)
  • Author(s): 岩部寛之, 埴岡伸光, 成松鎭雄
  • Organizer: 日本薬学会第127年会
  • Place of Presentation: 富山
  • Year and Date: 2007-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カニクイザルUGT2B9の酵素化学的特性-ヒトUGT2B7との比較- (2007)
  • Author(s): 重田尚美, 志賀里美, 山本真紀, 林啓子, 竹田友理, 埴岡伸光, 内藤真策, 清水剛文, 成松鎭雄
  • Organizer: 日本薬学会第127年会
  • Place of Presentation: 富山
  • Year and Date: 2007-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Expression and inducibility of drug-metabolizing enzymes in MCF-7 cells (2007)
  • Author(s): Iwabu, H., Hanioka, N., Narimatsu, S
  • Organizer: 127th Annual Meeting of the Pharmaceutical Society of Japan
  • Place of Presentation: Toyama, Japan
  • Year and Date: 2007-03-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Characterization of cynomolgus monkey UGT2B9 : comparison with human UGT2B7 (2007)
  • Author(s): Shigeta, N., Shiga, S., Yamamoto, M., Hayashi, K., Takeda, Y., Hanioka, N., Naito, S., Shimizu, T., Narimatsu, S
  • Organizer: 127th Annual Meeting of the Pharmaceutical Society of Japan
  • Place of Presentation: Toyama, Japan
  • Year and Date: 2007-03-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Functional characterization of human and cynomolgus monkey cytochrome P450 2E1 enzymes (2007)
  • Author(s): Yamanoto, M., Yonemoto, R, Iwabu, H., Masuda, K., Katsu, T., Hanioka, N., Tanaka-Kagawa, T., Jinno, H., Naito, S., Shimizu, T., Narimatsu, S
  • Organizer: 127th Annual Meeting of the Pharmaceutical Society of Japan
  • Place of Presentation: Toyama, Japan
  • Year and Date: 2007-03-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 日本人で新たに見出されたCYP2C19(CYP2C19^*18及びCYP2C19^*19)の機能解析 (2006)
  • Author(s): 經遠祐美, 埴岡伸光, 齋藤嘉朗, 角田知子, 前川京子, 齋藤啓太, 澤田純一, 成松鎭雄
  • Organizer: 日本薬物動態学会第21回年会
  • Place of Presentation: 東京
  • Year and Date: 2006-11-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Functional characterization of two novel CYP2C19 variants (CYP2C19*18 and CYP2CI9*19) found in a Japanese population (2006)
  • Author(s): Tsuneto, Y., Hanioka, N., Saito, Y., Sumada, T., Maekawa, K., Saito, K., Sawada, J., Narimatsu, S
  • Organizer: 21st Annual Meeting of Japanese Society for the Study of Xenobiotics
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2006-11-30
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 内分泌攪乱化学物質ビスフェノールAとヒトおよびカニクイザルUDP-グルクロン酸転移酵素1A6の相互作用 (2006)
  • Author(s): 竹田友理, 香川(田中)聡子, 神野透人, 内藤真策, 埴岡伸光, 成松鎭雄
  • Organizer: フォーラム2006:衛生薬学・環境トキシコロジー
  • Place of Presentation: 東京
  • Year and Date: 2006-10-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カニクイザルCYP2E1の酵素機能:ヒト酵素との比較 (2006)
  • Author(s): 山本真紀, 米本麗, 増田和文, 勝孝, 埴岡伸光, 内藤真策, 成松鎭雄
  • Organizer: フォーラム2006:衛生薬学・環境トキシコロジー
  • Place of Presentation: 東京
  • Year and Date: 2006-10-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Interaction of bisphenol A with human and cynomolgus monkey UDP-glucuronosyltransferase 1A6 enzyms (2006)
  • Author(s): Takeda, Y., Tanaka-Kagawa, T., Jinno, H., Naito, S., Hanioka, N., Narimatsu, S
  • Organizer: Forum 2006 : Pharmaceutical Health Science and Environmental Toxicology
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2006-10-30
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Characterization of cynomolgus monkey CYP2E1 : comparison with human CYP2E1 (2006)
  • Author(s): Yamanoto, M., Yonemoto, R., Masuda, K., Katsu, T., Hanioka, N., Naito, S., Narimatsu, S
  • Organizer: Forum 2006 : Pharmaceutical Health Science and Environmental Toxicology
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2006-10-30
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ヒト肺上皮細胞株A549における薬物代謝酵素の発現・誘導性 (2006)
  • Author(s): 岩部寛之, 埴岡伸光, 成松鎭雄
  • Organizer: 第45回日本薬学会・日本病院薬剤師会中国四国支部学術大会
  • Place of Presentation: 広島
  • Year and Date: 2006-10-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 酵母発現ベクターpYESを用いたCYP-fp2共発現系の構築 (2006)
  • Author(s): 辻さや香, 岩部寛之, 山本真紀, 竹田友理, 鳥越史宙, 埴岡伸光, 成松鎭雄
  • Organizer: 第45回日本薬学会・日本病院薬剤師会中国四国支部学術大会
  • Place of Presentation: 広島
  • Year and Date: 2006-10-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 日本人で新たに見出された変異型CYP1A2の機能解析 (2006)
  • Author(s): 經遠祐美, 礒部隆史, 埴岡伸光, 齋藤嘉朗, 前川京子, 香川(田中)聡子, 神野透人, 澤田純一, 成松鎭雄
  • Organizer: 第45回日本薬学会・日本病院薬剤師会中国四国支部学術大会
  • Place of Presentation: 広島
  • Year and Date: 2006-10-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Expression and inducibility of drug-metabolizing enzymes in A549 cells (2006)
  • Author(s): Iwabu, H., Hanioka, N., Narimatsu, S
  • Organizer: 45th Annual Meeting of Chugoku-Shikoku Branch of the Pharmaceutical Society of Japan
  • Place of Presentation: Hiroshima, Japan
  • Year and Date: 2006-10-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Construction of coexpression of CYP-fp2 using yeast expression vector pYES (2006)
  • Author(s): Tsuji, S., Iwabu, H., M., Yamamoto, Takeda, Y., Torigoe, F., Hanioka, N., Narimatsu, S
  • Organizer: 45th Annual Meeting of Chugoku-Shikoku Branch of the Pharmaceutical Society of Japan
  • Place of Presentation: Hiroshima, Japan
  • Year and Date: 2006-10-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Functional analysis of three CYP1A2 variants found in a Japanese population (2006)
  • Author(s): Tsuneto, Y., Isobe, T., Hanioka, N., Saito, Y., Maekawa, K., Tanaka-Kagawa, T., Jinno, H., Sawada, J., Narimatsu, S
  • Organizer: 45th Annual Meeting of Chugoku-Shikoku Branch of the Pharmaceutical Society of Japan
  • Place of Presentation: Hiroshima, Japan
  • Year and Date: 2006-10-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ヒトおよびカニクイザルのUDP-グルクロン酸転移酵素1A6分子種の機能解析 (2006)
  • Author(s): 竹田友理, 埴岡伸光, 成松鎭雄
  • Organizer: 第47回日本生化学会中国・四国支部例会
  • Place of Presentation: 松江
  • Year and Date: 2006-05-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Functional characterization of human and cynomolgus monkey UDP- glucuronosyltransferase lA6 enzymes (2006)
  • Author(s): Takeda, Y., Hanioka, N., Narimatsu, S
  • Organizer: 47th Annual Meeting of Chugoku-Shikoku Branch of the Japanese Biochemical Society
  • Place of Presentation: Matsue, Japan
  • Year and Date: 2006-05-12
  • Description: 「研究成果報告書概要(欧文)」より